Improved expression of Rhizopus oryzae α-amylase in the methylotrophic yeast Pichia pastoris

Protein Expression and Purification

Volumn 79, Issue 1, 2011, Pages 142-148

  Li, Song ^a   Sing, Suren ^b   Wang, Zhengxiang ^a  

^a JIANGNAN UNIVERSITY   (China)

^b DURBAN UNIVERSITY OF TECHNOLOGY   (South Africa)

Author keywords

Amylase; Pichia pastoris; Promoter; Protein; Rhizopus oryzae; Signal sequence

Indexed keywords

AMYLASE; RECOMBINANT PROTEIN; STARCH;

ARTICLE; ENZYMOLOGY; GENE DOSAGE; GENE EXPRESSION REGULATION; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PICHIA; RHIZOPUS;

ALPHA-AMYLASES; CLONING, MOLECULAR; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; PICHIA; RECOMBINANT PROTEINS; RHIZOPUS; STARCH; TRANSFORMATION, GENETIC;

ESCHERICHIA COLI; PICHIA PASTORIS; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE;

EID: 79960142083     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.05.007     Document Type: Article

References (32)

1. M.J. van der Maarel, B. van der Veen, J.C. Uitdehag, H. Leemhuis, and L. Dijkhuizen Properties and applications of starch-converting enzymes of the α-amylase family J. Biotechnol. 94 2002 137 155 (Pubitemid 34127657)
2. R. Gupta, P. Gigras, H. Mohapatra, V.K. Goswami, and B. Chauhan Microbial α-amylases: a biotechnological perspective Process Biochem. 38 2003 1599 1616
3. T. Suganuma, K. Fujita, and K. Kitahara Some distinguishable properties between acid-stable and neutral types of α-amylases from acid-producing Koji J. Biosci. Bioeng. 104 2007 353 362 (Pubitemid 350251289)
4. J.H. Kim, T. Maeda, and N. Morita Effect of α-fungal amylase on the dough properties and bread quality of wheat flour substituted with polished flours Food Res. Int. 39 2006 117 126 (Pubitemid 41455499)
5. S. Sivaramakrishnan, D. Gangadharan, K.M. Nampoothiri, C.R. Soccol, and A. Pandey α-Amylase from microbial sources - an overview on recent developments Food Technol. Biotechnol. 44 2006 173 184 (Pubitemid 44033841)
6. M. Carlsen, J. Nielsen, and J. Villadsen Kinetic studies of acid-inactivation of α-amylase from Aspergillus oryzae Chem. Eng. Sci. 51 1996 37 43
7. X.L. Yuan, R.M. van der Kaaij, C.A. van den Hondel, P.J. Punt, M.J. van der Maarel, L. Dijkhuizen, and A.F. Ram Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles Mol. Genet. Genom. 279 2008 545 561
8. E. Dannaoui, J.F.G.M. Meis, D. Loebenberg, and P.E. Verweij Activity of pasaconazole in treatment of experimental disseminated zygomycosis Antimicrob. Agents Chemother. 47 2003 3647 3650 (Pubitemid 37345740)
9. C.R. Soccol, I. Iloki, B. Marin, and M. Raimbault Comparative production of alpha-amylase, glucoamylase and protein enrichment of raw and cooked cassava by Rhizopus strains in submerged and solid state fermentations J. Food Sci. Technol. 31 1994 320 323
10. J.A. Mertens, and C.D. Skory Isolation and characterization of a second glucoamylase gene without a starch binding domain from Rhizopus oryzae Enzyme Microb. Technol. 40 2007 874 880 (Pubitemid 46329080)
11. J.L. Cereghino, and J.M. Cregg Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiol. Rev. 24 2000 45 66 (Pubitemid 30047114)
12. R. Daly, and M.T.W. Hearn Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production J. Mol. Recognit. 18 2005 119 138 (Pubitemid 40328574)
13. D.R. Higgins, Overview of protein expression in Pichia pastoris, in: P.T. Wingfiled (Ed.), Curr. Protoc. Protein Sci., Supplement 2, Wiley, New York, 1995, pp. 5.7.1-5.7.16.
14. S. Kato, M. Ishibashi, D. Tatsuda, H. Tokunaga, and M. Tokunaga Efficient expression, purification and characterization of mouse salivary alpha-amylase secreted from methylotrophic yeast, Pichia pastoris Yeast 18 2001 643 655 (Pubitemid 32496340)
15. N. Juge, J.S. Andersen, D. Tull, P. Roepstorff, and B. Svensson Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris Protein Expr. Purif. 8 1996 204 214 (Pubitemid 26355084)
16. E. Paifer, E. Margolles, J. Cremata, R. Montesino, L. Herrera, and J.M. Delgado Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences Yeast 10 1994 1415 1419 (Pubitemid 24347994)
17. B. Karakas, M. Inan, and M. Certel Expression and characterization of Bacillus subtilis PY22 α-amylase in Pichia pastoris J. Mol. Catal. B: Enzym. 64 2010 129 134
18. Invitrogen, Pichia expression kit - a manual of methods for expression of recombinant proteins in Pichia pastoris, Catalog No. K1710-01.
19. Invitrogen, Pichia expression vectors for constitutive expression and purification of recombinant proteins, Catalog Nos. V200-20 and V205-20.
20. S. Abad, K. Kitz, A. Hormann, U. Schreiner, F.S. Hartner, and A. Glieder Real-time PCR-based determination of gene copy numbers in Pichia pastoris Biotechnol. J. 5 2010 413 420
21. Invitrogen, Pichia fermentation process guidelines, Version B, 2002.
22. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
23. C.R. Merril Gel-staining techniques M.P. Deutscher, Guide to Protein Purification 1990 Academic Press Inc. San Diego 477 488 (Pubitemid 20154409)
24. G.L. Miller Use of dinitrosalicylic acid reagent for determination of reducing sugar Anal. Chem. 31 1959 426 429
25. J. Lin-Cereghino, M.D. Hashimoto, A. Moy, J. Castelo, C.C. Orazem, P. Kuo, S. Xiong, V. Gandhi, C.T. Hatae, A. Chan, and G.P. Lin-Cereghino Direct selection of Pichia pastoris expression strains using new G418 resistance vectors Yeast 25 2008 293 299 (Pubitemid 351577731)
26. H. Marx, A. Mecklenbrauker, B. Gasser, M. Sauer, and D. Mattanovich Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus FEMS Yeast Res. 9 2009 1260 1270
27. J.M. Wu, J.C. Lin, L.L. Chieng, C.K. Lee, and T.A. Hsu Combined use of GAP and AOX1 promoter to enhance the expression of human granulocyte-macrophage colony-stimulating factor in Pichia pastoris Enzyme Microb. Technol. 33 2003 453 459 (Pubitemid 37011395)
28. X.P. He, N. Liu, W.W. Li, Z.Y. Zhang, B. Zhang, and Y.H. Ma Inducible and constitutive expression of a novel thermostable alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5 in Pichia pastoris and characterization of the recombinant enzyme Enzyme Microb. Technol. 43 2008 13 18
29. A. Ghosalkar, V. Sahai, and A. Srivastava Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals Protein Expr. Purif. 60 2008 103 109
30. J.G. Berrin, G. Williamson, A. Puigserver, J.C. Chaix, W.R. McLauchlan, and N. Juge High-level production of recombinant fungal endo-β-1,4-xylanase in the methylotrophic yeast Pichia pastoris Protein Expr. Purif. 19 2000 179 187 (Pubitemid 30387909)
31. W. Martinet, M. Maras, X. Saelens, W.M. Jou, and R. Contreras Modification of the protein glycosylation pathway in the methylotrophic yeast Pichia pastoris Biotechnol. Lett. 20 1998 1171 1177 (Pubitemid 29034193)
32. E. Rodriguez, Z.A. Wood, P.A. Karplus, and X.G. Lei Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris Arch. Biochem. Biophys. 382 2000 105 112