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Volumn 382, Issue 1, 2000, Pages 105-112

Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris

(4) Rodriguez, Eric a Wood, Zachary A b Karplus, P Andrew b Lei, Xin Gen a

a Department of Obstetrics Gynecology (United States)

b OREGON STATE UNIVERSITY (United States)

Author keywords

Catalytic function; Escherichia coli acid phosphatase; Glycosylation; Heterologous expression; Mutagenesis; Phytase; Pichia pastoris; Protein structure; Thermostability

Indexed keywords

ACID PHOSPHATASE; BACTERIAL ENZYME; PHYTASE;

CATALYSIS; CONFERENCE PAPER; CRYSTAL STRUCTURE; ENZYME STRUCTURE; ESCHERICHIA COLI; GLYCOSYLATION; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FELIS CATUS; PICHIA PASTORIS;

EID: 0034307020 PISSN: 00039861 EISSN: None Source Type: Journal
DOI: 10.1006/abbi.2000.2021 Document Type: Conference Paper

Times cited : (110)

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