Functional characterization of rat plasma membrane monoamine transporter in the blood-brain and blood-cerebrospinal fluid barriers

Journal of Pharmaceutical Sciences

Volumn 100, Issue 9, 2011, Pages 3924-3938

  Okura, Takashi ^a   Kato, Sayaka ^a   Takano, Yusuke ^a   Sato, Takenori ^a   Yamashita, Atsushi ^a   Morimoto, Riyo ^a   Ohtsuki, Sumio ^b   Terasaki, Tetsuya ^b   Deguchi, Yoshiharu ^a  

^a TEIKYO UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

1 methyl 4 phenylpyridinium; Blood brain barrier; Blood cerebrospinal fluid barrier; CNS; In vitro models; Plasma membrane monoamine transporter; TR BBB13 cells; TR CSFB3 cells; Transporters

Indexed keywords

4 AMINOHIPPURIC ACID; AMANTADINE; CARNITINE; CARRIER PROTEIN; CATION; CHOLINE; DOPAMINE; GUANIDINE; HISTAMINE; MESSENGER RNA; NORADRENALIN; OCTAMER TRANSCRIPTION FACTOR 1; OCTAMER TRANSCRIPTION FACTOR 2; OCTAMER TRANSCRIPTION FACTOR 4; PLASMA MEMBRANE MONOAMINE TRANSPORTER; PROCAINAMIDE; QUINIDINE; SEROTONIN; SMALL INTERFERING RNA; TETRYLAMMONIUM; THIAMINE; UNCLASSIFIED DRUG; VERAPAMIL;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD BRAIN BARRIER; BLOOD CEREBROSPINAL FLUID BARRIER; BRAIN; BRAIN PERFUSION; CELLULAR DISTRIBUTION; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; MALE; MICRODIALYSIS; NONHUMAN; PROTEIN EXPRESSION; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

EID: 79960135864     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.22594     Document Type: Article

References (44)

1. Pardridge WM. 2007. Pharm Res 24:1733-1744.
2. Cecchelli R, Berezowski V, Lundquist S, Culot M, Renftel M, Dehouck MP, Fenart L. 2007. Modeling of the blood-brain barrier in drug discovery and development. Nat Rev Drug Discov 6:650-661.
3. Ohtsuki S, Terasaki T. 2007. Contribution of carrier-mediated transport systems to the blood-brain barrier as a supporting and protecting interface for the brain; importance for CNS drug discovery and development. Pharm Res 24:1745-1758.
4. Tsuji A. 2005. Small molecular drug transfer across the blood-brain barrier via carrier-mediated transport systems. NeuroRx 2:54-62.
5. Wakayama K, Ohtsuki S, Takanaga H, Hosoya K, Terasaki T. 2002. Localization of norepinephrine and serotonin transporter in mouse brain capillary endothelial cells. Neurosci Res 44:173-180.
6. Tochino Y, Schanker LS. 1965. Transport of serotonin and norepinephrine by the rabbit choroid plexus in vitro. Biochem Pharmacol 14:1557-1566.
7. Hardebo JE, Owman C. 1980. Characterization of the in vitro uptake of monoamines into brain microvessels. Acta Physiol Scand 108:223-229.
8. Brust P, Friedrich A, Krizbai IA, Bergmann R, Roux F, Ganapathy V, Johannsen B. 2000. Functional expression of the serotonin transporter in immortalized rat brain microvessel endothelial cells. J Neurochem 74:1241-1248.
9. Engel K, Zhou M, Wang J. 2004. Identification and characterization of a novel monoamine transporter in the human brain. J Biol Chem 279:50042-50049.
10. Engel K, Wang J. 2005. Interaction of organic cations with a newly identified plasma membrane monoamine transporter. Mol Pharmacol 68:1397-1407.
11. Dahlin A, Xia L, Kong W, Hevner R, Wang J. 2007. Expression and immunolocalization of the plasma membrane monoamine transporter in the brain. Neuroscience 146:1193-1211.
12. Vialou V, Balasse L, Dumas S, Giros B, Gautron S. 2007. Neurochemical characterization of pathways expressing plasma membrane monoamine transporter in the rat brain. Neuroscience 144:616-622.
13. Buck KJ, Amara SG. 1994. Chimeric dopamine-norepinephrine transporters delineate structural domains influencing selectivity for catecholamines and 1-methyl-4-phenylpyridinium. Proc Natl Acad Sci U S A 91:12584-12588.
14. Sitte HH, Hiptmair B, Zwach J, Pifl C, Singer EA, Scholze P. 2001. Quantitative analysis of inward and outward transport rates in cells stably expressing the cloned human serotonin transporter: Inconsistencies with the hypothesis of facilitated exchange diffusion. Mol Pharmacol 59:1129-1137.
15. Terasaki T, Ohtsuki S, Hori S, Takanaga H, Nakashima E, Hosoya K. 2003. New approaches to in vitro models of blood-brain barrier drug transport. Drug Discov Today 8:944-954.
16. Hosoya K, Takashima T, Tetsuka K, Nagura T, Ohtsuki S, Takanaga H, Ueda M, Yanai N, Obinata M, Terasaki T. 2000. mRNA expression and transport characterization of conditionally immortalized rat brain capillary endothelial cell lines; a new in vitro BBB model for drug targeting. J Drug Target 8:357-370.
17. Kitazawa T, Hosoya K, Watanabe M, Takashima T, Ohtsuki S, Takanaga H, Ueda M, Yanai N, Obinata M, Terasaki T. 2001. Characterization of the amino acid transport of new immortalized choroid plexus epithelial cell lines: A novel in vitro system for investigating transport functions at the blood-cerebrospinal fluid barrier. Pharm Res 18:16-22.
18. Ohtsuki S, Yamaguchi H, Asashima T, Terasaki T. 2007. Establishing a method to isolate rat brain capillary endothelial cells by magnetic cell sorting and dominant mRNA expression of multidrug resistance-associated protein 1 and 4 in highly purified rat brain capillary endothelial cells. Pharm Res 24:688-694.
19. Okura T, Hattori A, Takano Y, Sato T, Hammarlund-Udenaes M, Terasaki T, Deguchi Y. 2008. Involvement of the pyrilamine transporter, a putative organic cation transporter, in blood-brain barrier transport of oxycodone. Drug Metab Dispos 36:2005-2013.
20. Okura T, Ito R, Ishiguro N, Tamai I, Deguchi Y. 2007. Blood-brain barrier transport of pramipexole, a dopamine D2 agonist. Life Sci 80:1564-1571.
21. Hashimoto Y, Okiyoneda T, Harada K, Ueno K, Sugahara T, Yamashita A, Shuto T, Suico MA, Kai H. 2008. Phosphatidic acid metabolism regulates the intracellular trafficking and retrotranslocation of CFTR. Biochim Biophys Acta 1783:153-162.
22. Takasato Y, Rapoport SI, Smith QR. 1984. An in situ brain perfusion technique to study cerebrovascular transport in the rat. Am J Physiol 247: H484-H493.
23. Kakee A, Terasaki T, Sugiyama Y. Brain efflux index as a novel method of analyzing efflux transport at the blood-brain barrier. 1996. J Pharmacol Exp Ther 277:1550-1559.
24. Terasaki T, Deguchi Y, Kasama Y, Pardridge WM, Tsuji A. 1992. Determination of in vivo steady-state unbound drug concentration in the brain interstitial fluid by microdialysis. Int J Pharm 81:143-152.
25. Deguchi Y, Terasaki T, Kawasaki S, Tsuji A. 1991. Muscle microdialysis as a model study to relate the drug concentration in tissue interstitial fluid and dialysate. J Pharmacobiodyn 14:483-492.
26. Bungay PM, Morrison PF, Dedrick RL. 1990. Steady-state theory for quantitative microdialysis of solutes and water in vivo and in vitro. Life Sci 46:105-119.
27. Okura T, Saito M, Nakanishi M, Komiyama N, Fujii A, Yamada S, Kimura R. 2003. Different distribution of morphine and morphine-6 beta-glucuronide after intracerebroventricular injection in rats. Br J Pharmacol 140:211-217.
28. Allen DD, Smith QR. 2001. Characterization of the blood-brain barrier choline transporter using the in situ rat brain perfusion technique. J Neurochem 76:1032-1041.
29. Sriram K, Pai KS, Ravindranath V. 1995. Protection and potentiation of 1-methyl-4-phenylpyridinium-induced toxicity by cytochrome P450 inhibitors and inducer may be due to the altered uptake of the toxin. J Neurochem 64:1203-1208.
30. Keep RF, Jones HC. 1990. Cortical microvessels during brain development: A morphometric study in the rat. Microvasc Res 40:412-426.
31. Keep RF, Jones HC. 1990. A morphometric study on the development of the lateral ventricle choroid plexus, choroid plexus capillaries, and ventricular ependyma in the rat. Brain Res Dev Brain Res 56:47-53.
32. Kido Y, Tamai I, Ohnari A, Sai Y, Kagami T, Nezu J, Nikaido H, Hashimoto N, Asano M, Tsuji A. 2001. Functional relevance of carnitine transporter OCTN2 to brain distribution of l-carnitine and acetyl-l-carnitine across the blood-brain barrier. J Neurochem 79:959-969.
33. Dahlin A, Royall J, Hohmann JG, Wang J. 2009. Expression profiling of the solute carrier gene family in the mouse brain. J Pharmacol Exp Ther 329:558-570.
34. Xia L, Engel K, Zhou M, Wang J. 2007. Membrane localization and pH-dependent transport of a newly cloned organic cation transporter (PMAT) in kidney cells. Am J Physiol Renal Physiol 292:F682-F690.
35. Mutch WA, Hansen AJ. 1984. Extracellular pH changes during spreading depression and cerebral ischemia: Mechanisms of brain pH regulation. J Cereb Blood Flow Metab 4:17-27.
36. Arndt P, Volk C, Gorboulev V, Budiman T, Popp C, Ulzheimer-Teuber I, Akhoundova A, Koppatz S, Bamberg E, Nagel G, Koepsell H. 2001. Interaction of cations, anions, and weak base quinine with rat renal cation transporter rOCT2 compared with rOCT1. Am J Physiol Renal Physiol 281:F454-F468.
37. Wu X, Kekuda R, Huang W, Fei YJ, Leibach FH, Chen J, Conway SJ, Ganapathy V. 1998. Identity of the organic cation transporter OCT3 as the extraneuronal monoamine transporter (uptake2) and evidence for the expression of the transporter in the brain. J Biol Chem 273:32776-32786.
38. Streich S, Brüss M, Bönisch H. 1996. Expression of the extraneuronal monoamine transporter (uptake2) in human glioma cells. Naunyn Schmiedebergs Arch Pharmacol 353:328-333.
39. Joyce JN, Woolsey C, Ryoo H, Borwege S, Hagner D. 2004. Low dose pramipexole is neuroprotective in the MPTP mouse model of Parkinson's disease, and downregulates the dopamine transporter via the D3 receptor. BMC Biol 2:22.
40. Rodríguez GJ, Roman DL, White KJ, Nichols DE, Barker EL. 2003. Distinct recognition of substrates by the human and Drosophila serotonin transporters. J Pharmacol Exp Ther 306:338-346.
41. Ohashi R, Tamai I, Nezu Ji J, Nikaido H, Hashimoto N, Oku A, Sai Y, Shimane M, Tsuji A. 2001. Molecular and physiological evidence for multifunctionality of carnitine/organic cation transporter OCTN2. Mol Pharmacol 59:358-366.
42. Sweet DH, Miller DS, Pritchard JB. 2001. Ventricular choline transport: A role for organic cation transporter 2 expressed in choroid plexus. J Biol Chem 276:41611-41619.
43. Pardridge WM. 2005. Molecular biology of the blood-brain barrier. Mol Biotechnol 30:57-70.
44. Kotake Y, Ohta S. 2003. MPP+ analogs acting on mitochondria and inducing neuro-degeneration. Curr Med Chem 10:2507-2516.