Diversity of culturable psychrophilic and psychrotrophic anaerobic bacteria isolated from beef abattoirs and their environments

Applied and Environmental Microbiology

Volumn 77, Issue 13, 2011, Pages 4280-4284

  Moschonas, G ^a,b   Bolton, D J ^a   McDowell, D A ^b   Sheridan, J J ^a  

^a ASHTOWN FOOD RESEARCH CENTRE   (Ireland)

^b UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA; ANAEROBIC BACTERIUM; ANAEROBIC MICROFLORA; CULTURABLE; GENETIC DIVERSITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS;

CLOSTRIDIUM; RNA; SPOILAGE; VACUUM;

MEATS;

BACTERIAL DNA; RIBOSOME DNA; RNA 16S;

ANOXIC CONDITIONS; BACTERIUM; FOOD QUALITY; GENETIC ANALYSIS; INTRASPECIFIC VARIATION; LIVESTOCK; LOW TEMPERATURE; MEAT; POLYMERASE CHAIN REACTION; SPECIES DIVERSITY;

ANAEROBIC BACTERIUM; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; CLASSIFICATION; CLUSTER ANALYSIS; COLD; DNA SEQUENCE; GENETICS; GROWTH, DEVELOPMENT AND AGING; IRELAND; ISOLATION AND PURIFICATION; MEAT; METABOLISM; MICROBIOLOGY; MOLECULAR TYPING; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; SLAUGHTERHOUSE;

ABATTOIRS; ANIMALS; BACTERIA, ANAEROBIC; CATTLE; CLUSTER ANALYSIS; COLD TEMPERATURE; DNA, BACTERIAL; DNA, RIBOSOMAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENVIRONMENTAL MICROBIOLOGY; IRELAND; MEAT; MOLECULAR TYPING; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

CLOSTRIDIUM ESTERTHETICUM; CLOSTRIDIUM GASIGENES;

EID: 79960077690     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01778-10     Document Type: Article

References (37)

1. Boerema, J. A., D. M. Broda, and R. G. Bell. 2003. Abattoir sources of psychrophilic clostridia causing blown pack spoilage of vacuum-packed chilled meats determined by culture-based and molecular detection procedures. Lett. Appl. Microbiol. 36:406-411.
2. Brambilla, E., H. Hippe, A. Hagelstein, B. J. Tindall, and E. Stackebrandt. 2001. 16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, McMurdo Dry Valleys, Antarctica. Extremophiles 5:23-33.
3. Broda, D., D. Saul, R. Bell, and D. Musgrave. 2000. Clostridium algidixylanolyticum sp. nov., a psychrotolerant, xylan-degrading, spore-forming bacterium. Int. J. Syst. Evol. Microbiol. 50:623-631.
4. Broda, D., D. Saul, P. Lawson, R. Bell, and D. Musgrave. 2000. Clostridium gasigenes sp. nov., a psychrophile causing spoilage of vacuum-packed meat. Int. J. Syst. Evol. Microbiol. 50:107-118.
5. Broda, D. M., R. G. Bell, J. A. Boerema, and D. R. Musgrave. 2002. The abattoir source of culturable psychrophilic Clostridium spp. causing 'blown pack' spoilage of vacuum-packed chilled venison. J. Appl. Microbiol. 93:817- 824.
6. Broda, D. M., J. A. Boerema, and R. G. Bell. 2003. PCR detection of psychrophilic Clostridium spp. causing 'blown pack' spoilage of vacuumpacked chilled meats. J. Appl. Microbiol. 94:515-522.
7. Broda, D. M., J. A. Boerema, and G. Brightwell. 2009. Sources of psychrophilic and psychrotolerant clostridia causing spoilage of vacuum-packed chilled meats, as determined by PCR amplification procedure. J. Appl. Microbiol. 107:178-186.
8. Broda, D. M., D. R. Musgrave, and R. G. Bell. 2003. Molecular differentiation of clostridia associated with 'blown pack' spoilage of vacuum-packed meats using internal transcribed spacer polymorphism analysis. Int. J. Food Microbiol. 84:71-77.
9. Broda, D. M., D. R. Musgrave, and R. G. Bell. 2000. Use of restriction fragment length polymorphism analysis to differentiate strains of psychrophilic and psychrotrophic clostridia associated with 'blown pack' spoilage of vacuum-packed meats. J. Appl. Microbiol. 88:107-116.
10. Clarridge, J. E., III. 2004. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin. Microbiol. Rev. 17:840-862.
11. Clementino, M. M., et al. 2001. PCR analyses of tRNA intergenic spacer, 16S-23S internal transcribed spacer, and randomly amplified polymorphic DNA reveal inter- and intraspecific relationships of Enterobacter cloacae strains. J. Clin. Microbiol. 39:3865-3870.
12. Collins, M. D., et al. 1994. The phylogeny of the genus Clostridium-proposal of five new genera and eleven new species combinations. Int J. Syst. Bacteriol. 44:812-826.
13. Collins, M. D., U. M. Rodrigues, R. H. Dainty, R. A. Edwards, and T. A. Roberts. 1992. Taxonomic studies on a psychrophilic Clostridium from vacuum-packed beef: description of Clostridium estertheticum sp. nov. FEMS Microbiol. Lett. 75:235-240.
14. Dahlenborg, M., E. Borch, and P. Rådström. 2003. Prevalence of Clostridium botulinum types B, E and F in faecal samples from Swedish cattle. Int. J. Food Microbiol. 82:105-110.
15. Dainty, R. H., R. A. Edwards, and C. M. Hibbard. 1989. Spoilage of vacuumpacked beef by a Clostridium sp. J. Sci. Food Agric. 49:473-486.
16. Garcia-Martinez, J., S. G. Acinas, A. I. Anton, and F. Rodriguez-Valera. 1999. Use of the 16S-23S ribosomal genes spacer region in studies of prokaryotic diversity. J. Microbiol. Methods 36:55-64.
17. Gupta, A., C. J. Sumner, M. Castor, S. Maslanka, and J. Sobel. 2005. Adult botulism type F in the United States, 1981-2002. Neurology 65:1694-1700.
18. Helps, C. R., D. A. Harbour, and J. E. L. Corry. 1999. PCR-based 16S ribosomal DNA detection technique for Clostridium estertheticum causing spoilage in vacuum-packed chill-stored beef. Int. J. Food Microbiol. 52: 57-65.
19. Hippe, H., J. R. Andreesen, and G. Gottschalk. 1992. The genus Clostridium-nonmedical, p. 1800-1866. In A. Balows et al. (ed.), The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications. Springer, New York, NY.
20. Jiang, W., S. Abrar, M. Romagnoli, and K. C. Carroll. 2009. Clostridium glycolicum wound infections: case reports and review of the literature. J. Clin. Microbiol. 47:1599-1601.
21. Kalchayanand, N., B. Ray, R. A. Field, and M. C. Johnson. 1989. Spoilage of vacuum-packaged refrigerated beef by Clostridium. J. Food Prot. 52:424-426.
22. Kawasaki, S., P. A. Fratamico, I. V. Wesley, and S. Kawamotol. 2008. Species-specific identification of campylobacters by PCR-restriction fragment length polymorphism and PCR targeting of the gyrase B gene. Appl. Environ. Microbiol. 74:2529-2533.
23. Lund, B. M., A. F. Graham, S. M. George, and D. Brown. 1990. The combined effect of incubation temperature, pH and sorbic acid on the probability of growth of nonproteolytic, type B Clostridium botulinum. J. Appl. Bacteriol. 69:481-492.
24. Moschonas, G., D. J. Bolton, J. J. Sheridan, and D. A. McDowell. 2009. The effect of storage temperature and inoculum level on the time of onset of 'blown pack' spoilage. J. Appl. Microbiol. 108:532-539.
25. Moschonas, G., D. J. Bolton, J. J. Sheridan, and D. A. McDowell. 2009. Isolation and sources of 'blown pack' spoilage clostridia in beef abattoirs. J. Appl. Microbiol. 107:616-624.
26. Mullane, N. R., P. Whyte, P. G. Wall, T. Quinn, and S. Fanning. 2007. Application of pulsed-field gel electrophoresis to characterise and trace the prevalence of Enterobacter sakazakii in an infant formula processing facility. Int. J. Food Microbiol. 116:73-81.
27. Pond, M. J., D. M. Stone, and D. J. Alderman. 2006. Comparison of conventional and molecular techniques to investigate the intestinal microflora of rainbow trout (Oncorhynchus mykiss). Aquaculture 261:194-203.
28. Pot, B., P. Vandamme, and K. Kersters. 1994. Analysis of electrophoretic whole organism protein fingerprints, p. 493-521. In M. Goodfellow and A. G. O'Donnell (ed.), Chemical methods in prokaryotic systematics, vol. 5. John Wiley & Sons, Chichester, United Kingdom.
29. Relman, D. A. 1993. Universal bacterial 16S rDNA amplification and sequencing, p. 489-496. In D. H. Persing, T. F. Smith, F. C. Tenover, and T. J. White (ed.), Diagnostic molecular microbiology: principles and applications. American Society for Microbiology, Washington, DC.
30. Roth, A., et al. 1998. Differentiation of phylogenetically related slowly growing mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences. J. Clin. Microbiol. 36:139-147.
31. Seurinck, S., W. Verstraete, and S. D. Siciliano. 2003. Use of 16S-23S rRNA intergenic spacer region PCR and repetitive extragenic palindromic PCR analyses of Escherichia coli isolates to identify nonpoint fecal sources. Appl. Environ. Microbiol. 69:4942-4950.
32. Song, Y. L., et al. 2002. Use of 16S-23S rRNA spacer-region (SR)-PCR for identification of intestinal clostridia. Syst. Appl. Microbiol. 25:528-535.
33. Spring, S., et al. 2003. Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov. Int. J. Syst. Evol. Microbiol. 53:1019-1029.
34. Stackebrandt, E., and H. Hippe. 2005. Taxonomy and Systematics, p. 19-48. In P. D. H. Bahl (ed.), Clostridia. Biotechnology and medical applications. Wiley-VCH, Weinheim, Germany.
35. Tindall, B. J., et al. 2000. Cultivatable microbial biodiversity: gnawing at the Gordian knot. Environ. Microbiol. 2:310-318.
36. Woo, P. C. Y., S. K. P. Lau, J. L. L. Teng, H. Tse, and K. Y. Yuen. 2008. Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin. Microbiol. Infect. 14:908-934.
37. Yang, X. Q., C. O. Gill, and S. Balamurugan. 2010. Products of glucose and lactate fermentation, and utilization of amino acids by Clostridium estertheticum subsp. laramiense and estertheticum growing in meat juice medium. J. Food Prot. 73:1348-1352.