Sources of psychrophilic and psychrotolerant clostridia causing spoilage of vacuum-packed chilled meats, as determined by PCR amplification procedure

Journal of Applied Microbiology

Volumn 107, Issue 1, 2009, Pages 178-186

  Brightwell, G ^c   Broda, D M ^a   Boerema, J A ^b  

^a Inghams Enterprises ^*  (New Zealand)

^b Inghams Enterprises (NZ) Limited   (New Zealand)

^c RUAKURA RESEARCH CENTRE   (New Zealand)

Author keywords

Clostridia; Meat spoilage; Molecular detection

Indexed keywords

CLOSTRIDIUM; ESTERS; FLOORS; MEATS; SPOILAGE;

16S RDNA; ENVIRONMENTAL SAMPLE; FAECAL SAMPLES; MEAT SPOILAGE; MICRO-ORGANISMS; MOLECULAR DETECTION; MOLECULAR METHODS; PROCESSING PLANTS; PSYCHROTOLERANT; STOCK SUPPLIES;

POLYMERASE CHAIN REACTION;

DNA 16S;

BACTERIUM; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIUM DETECTION; CLOSTRIDIA; CLOSTRIDIUM ALGIDICARNIS; CLOSTRIDIUM ESTERTHETICUM; CLOSTRIDIUM GASIGENES; CLOSTRIDIUM PUTREFACIENS; CONTROLLED STUDY; FECES; FOOD SPOILAGE; HIGH TEMPERATURE PROCEDURES; MEAT INDUSTRY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PSYCHROPHILIC BACTERIUM; SLAUGHTERHOUSE; SPECIES DIFFERENTIATION; WOOL;

CLOSTRIDIA; CLOSTRIDIUM ALGIDICARNIS; CLOSTRIDIUM ESTERTHETICUM; CLOSTRIDIUM GASIGENES; CLOSTRIDIUM PUTREFACIENS;

EID: 66849104165     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04193.x     Document Type: Article

References (25)

1. Alam, S.I., Dixit, A., Reddy, G.S.N., Dube, S., Palit, M., Shivaji, S. Singh, L. (2006) Clostridium schirmacherense sp. nov., an obligately anaerobic, proteolytic, psychrophilic bacterium isolated from lake sediment of Schirmacher Oasis, Antarctica. Int J Syst Evol Microbiol 56, 715 720.
2. Boerema, J.A., Broda, D.M. Bell, R.G. (2002) PCR detection of psychrotolerant clostridia associated with deep tissue spoilage of vacuum-packed chilled meats. Lett Appl Microbiol 35, 446 450. (Pubitemid 35253724)
3. Boerema, J.A., Broda, D.M. Bell, R.G. (2003) Abattoir sources of psychrophilic clostridia causing 'blown pack' spoilage of vacuum-packed chilled meats determined by culture-based and molecular detection procedures. Lett Appl Microbiol 36, 406 411. (Pubitemid 36649085)
4. Broda, D.M., De Lacy, K.M., Bell, R.G. Penney, N. (1996a) Association of psychrotrophic Clostridium spp. with deep tissue spoilage of chilled vacuum-packed lamb. Int J Food Microbiol 29, 371 378. (Pubitemid 26185356)
5. Broda, D.M., De Lacy, K.M., Bell, R.G., Braggins, J.T. Cook, R.L. (1996b) Psychrotrophic Clostridium spp. associated with 'blown pack' spoilage of chilled vacuum-packed red meats and dog rolls in gas-impermeable plastic casings. Int J Food Microbiol 29, 335 352. (Pubitemid 26187975)
6. Broda, D.M., Musgrave, D.R. Bell, R.G. (2000a) Use of restriction fragment length polymorphism analysis to differentiate strains of psychrophilic and psychrotrophic clostridia associated with 'blown pack' spoilage of vacuum-packed meats. J Appl Microbiol 88, 107 116. (Pubitemid 30140188)
7. Broda, D.M., Saul, D.J., Lawson, P.A., Bell, R.G. Musgrave, D.R. (2000b) Clostridium gasigenes sp. nov., a psychrophile causing spoilage of vacuum-packed meat. Int J Syst Evol Microbiol 50, 107 118. (Pubitemid 30084061)
8. Broda, D.M., Boerema, J.A. Bell, R.G. (2003) PCR detection of psychrophilic Clostridium spp. causing 'blown pack' spoilage of vacuum-packed chilled meats. J Appl Microbiol 94, 515 522. (Pubitemid 36315043)
9. AL. In Bergey's Manual of Systematic Bacteriology, Vol. 2 ed. Sneath, P.H.A., Mair, N.S., Sharpe, M.E. Holt, J.G. pp. 1141 1200. Baltimore, MD : The Williams and Wilkins Co.
10. Collins, M.D., Rodrigues, U.M., Dainty, R.H., Edwards, R.A. Roberts, T.A. (1992) Taxonomic studies on a psychrophilic Clostridium from vacuum-packed beef: description of Clostridium estertheticum sp. nov. FEMS Microbiol Lett 96, 235 240.
11. Dainty, R.H., Edwards, R.A. Hibbard, C.M. (1989) Spoilage of vacuum-packed beef by a Clostridium sp. J Sci Food Agricult 49, 473 486.
12. Eisgruber, H. Stolle, A. (1996) Clostridia in red meat and slaughter animals. In Factors Affecting the Microbial Quality of Meat, Vol. 2. ed. Hinton, M.H. Rowlings, C. pp. 69 75. Bristol : University of Bristol Press.
13. Ercolani, G.L. (1997) Occurrence and persistence of culturable clostridial spores on the leaves of horticultural plants. J Appl Microbiol 82, 137 140. (Pubitemid 27226961)
14. Gill, C.O. (1979) Intrinsic bacteria in meat. J Appl Bacteriol 47, 367 378. (Pubitemid 10177577)
15. Gounot, A. (1991) Bacterial life at low temperature: physiological aspects and biotechnological implications. J Appl Bacteriol 71, 386 397. (Pubitemid 23395158)
16. Kalchayanand, N., Ray, B. Johnson, M.C. (1989) Spoilage of vacuum-packaged refrigerated beef by Clostridium. J Food Protect 52, 424 426.
17. Lawson, P., Dainty, R.H., Kristiansen, N., Berg, J. Collins, M.D. (1994) Characterization of a psychrotrophic Clostridium causing spoilage in vacuum-packaged cooked pork: description of Clostridium algidicarnis sp. nov. Lett Appl Microbiol 19, 153 157. (Pubitemid 24301381)
18. Lund, B.M. (1986) Anaerobes in relation to foods of plant origin. In Anaerobic Bacteria in Habitats Other than Man ed. Barnes, E.M. Mead, G.C. pp. 351 372. Oxford : Blackwell Scientific Publications.
19. Lund, B.M., Graham, A.F., George, S.M. Brown, D. (1990) The combined effect of incubation temperature, pH and sorbic acid on the probability of growth of non-proteolytic type B Clostridium botulinum. J Appl Bacteriol 69, 481 492. (Pubitemid 20359799)
20. Mountfort, D.O., Rainey, F.A., Burghardt, J., Kaspar, H.F. Stackebrandt, E. (1997) Clostridium vincentii sp. nov., a new obligately anaerobic, saccharolytic, psychrophilic bacterium isolated from low-salinity pond sediment of the McMurdo Ice Shelf, Antarctica. Arch Microbiol 167, 54 60. (Pubitemid 27089065)
21. Newton, K.G., Harrison, J.C.L. Wauters, A.M. (1978) Sources of psychrotrophic bacteria on meat at the abattoir. J Appl Bacteriol 45, 75 82. (Pubitemid 9010696)
22. Patterson, J.T. Gibbs, P.A. (1978) Sources and properties of some organisms isolated in two abattoirs. Meat Sci 2, 263 273.
23. Roberts, T.A. Mead, G.C 1986) Involvement of intestinal anaerobes in the spoilage of red meats, poultry and fish. In Anaerobic Bacteria in Habitats Other than Man ed. Barnes, E.M. Mead, G.C. pp. 333 349. Oxford : Blackwell Scientific Publications.
24. Ross, H.E. (1965) Clostridium putrefaciens: a neglected anaerobe. J Appl Bacteriol 28, 49 51.
25. Spring, S., Merkhoffer, B., Weiss, N., Kroppenstedt, R.M., Hippe, H. Stackebrandt, E. (2003) Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov. Int J Syst Evol Microbiol 53, 1019 1029. (Pubitemid 36896508)