Imaging mRNAs in Living Mammalian Cells

Methods in Molecular Biology

Volumn 714, Issue , 2011, Pages 249-263

  Yunger, Sharon ^b   Shav Tal, Yaron ^a  

^a BAR ILAN UNIVERSITY   (Israel)

^b NONE

Author keywords

Live cell imaging; Nucleus; RNA dynamics; Transcription

Indexed keywords

CAPSID PROTEIN; MESSENGER RNA;

ARTICLE; CELL SURVIVAL; FLUORESCENCE IN SITU HYBRIDIZATION; GENETICS; HELA CELL; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR IMAGING; NUCLEOTIDE REPEAT;

CAPSID PROTEINS; CELL SURVIVAL; HELA CELLS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MOLECULAR IMAGING; REPETITIVE SEQUENCES, NUCLEIC ACID; RNA, MESSENGER;

EID: 79960060061     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-005-8_16     Document Type: Chapter

References (21)

1. Lippincott-Schwartz, J., Altan-Bonnet, N., and Patterson, G. H. (2003) Photobleaching and photoactivation: following protein dynamics in living cells. Nat. Cell Biol. Suppl., S7–14.
2. Carmo-Fonseca, M., Platani, M., and Swedlow, J. R. (2002) Macromolecular mobility inside the cell nucleus. Trends Cell Biol. 12, 491–495.
3. Shav-Tal, Y., Darzacq, X., and Singer, R. H. (2006) Gene expression within a dynamic nuclear landscape. EMBO J. 25, 3469–3479.
4. Gorski, S. A., Dundr, M., and Misteli, T. (2006) The road much traveled: trafficking in the cell nucleus. Curr. Opin. Cell Biol. 18, 284 –290.
5. Janicki, S. M., Tsukamoto, T., Salghetti, S. E., Tansey, W. P., Sachidanandam, R., Prasanth, K. V., Ried, T., Shav-Tal, Y., Bertrand, E., Singer, R. H., and Spector, D. L. (2004) From silencing to gene expression; real-time analysis in single cells. Cell 116, 683 – 698.
6. Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S. M., Singer, R. H., and Long, R. M. (1998) Localization of ASH1 mRNA particles in living yeast. Mol. Cell 2, 437– 445.
7. Shav-Tal, Y., Darzacq, X., Shenoy, S. M., Fusco, D., Janicki, S. M., Spector, D. L., and Singer, R. H. (2004) Dynamics of single mRNPs in nuclei of living cells. Science 304, 1797–1800.
8. Darzacq, X., Shav-Tal, Y., de Turris, V., Brody, Y., Shenoy, S. M., Phair, R. D., and Singer, R. H. (2007) In vivo dynamics of RNA polymerase II transcription. Nat. Struct. Mol. Biol. 14, 796 – 806.
9. Lamond, A. I., and Swedlow, J. R. (2007) RNA polymerase II transcription in living color. Nat. Struct. Mol. Biol. 14, 788–790.
10. Wada, Y., Ohta, Y., Xu, M., Tsutsumi, S., Minami, T., Inoue, K., Komura, D., Kitakami, J., Oshida, N., Papantonis, A., Izumi, A., Kobayashi, M., Meguro, H., Kanki, Y., Mimura, I., Yamamoto, K., Mataki, C., Hamakubo, T., Shirahige, K., Aburatani, H., Kimura, H., Kodama, T., Cook, P. R., and Ihara, S. (2009) A wave of nascent transcription on activated human genes. Proc. Natl. Acad. Sci. U S A 106, 18357–18361.
11. Singh, J., and Padgett, R. A. (2009) Rates of in situ transcription and splicing in large human genes. Nat. Struct. Mol. Biol. 16, 1128 –1133.
12. Golding, I., and Cox, E. C. (2004) RNA dynamics in live Escherichia coli cells. Proc. Natl. Acad. Sci. U S A 101, 11310 –11315.
13. Golding, I., Paulsson, J., Zawilski, S. M., and Cox, E. C. (2005) Real-time kinetics of gene activity in individual bacteria. Cell 123, 1025–1036.
14. Chubb, J. R., Trcek, T., Shenoy, S. M., and Singer, R. H. (2006) Transcriptional pulsing of a developmental gene. Curr. Biol. 16, 1018–1025.
15. Elowitz, M. B., Levine, A. J., Siggia, E. D., and Swain, P. S. (2002) Stochastic gene expression in a single cell. Science 297, 1183–1186.
16. Ozbudak, E. M., Thattai, M., Kurtser, I., Grossman, A. D., and van Oudenaarden, A. (2002) Regulation of noise in the expression of a single gene. Nat. Genet. 31, 69 –73.
17. Shav-Tal, Y., and Gruenbaum, Y. (2009) Single-molecule dynamics of nuclear mRNA. F1000 Biology Reports 1, 29 – 32.
18. Boulon, S., Basyuk, E., Blanchard, J. M., Bertrand, E., and Verheggen, C. (2002) Intra-nuclear RNA trafficking: insights from live cell imaging. Biochimie 84, 805 – 813.
19. Yunger, S., Rosenfeld, L., Garini, Y., and Shav-Tal, Y. (2010) Single allele analysis of transcription kinetics in living mammalian cells. Nat. Methods 7, 631–633.
20. Mor, A., Suliman, S., Ben-Yishay, R., Yunger, S., Brody, Y., and Shav-Tal, Y. (2010) Dynamics of single mRNP nucleo-cytoplasmic transport through the nuclear pore in living cells. Nat. Cell Biol. 12, 543–552.
21. Fusco, D., Accornero, N., Lavoie, B., Shenoy, S. M., Blanchard, J. M., Singer, R. H., and Bertrand, E. (2003) Single mRNA molecules demonstrate probabilistic movement in living Mammalian cells. Curr. Biol. 13, 161–167.