Microbiology: Long-term monitoring of bacteria undergoing programmed population control in a microchemostat

Science

Volumn 309, Issue 5731, 2005, Pages 137-140

  Balagaddé, Frederick K ^a,c   You, Lingchong ^b,d   Hansen, Carl L ^a,e   Arnold, Frances H ^b   Quake, Stephen R ^a,c  

^a California Institute of Technology   (United States)

^b California Institute of Technology   (United States)

^c Stanford University   (United States)

^d Duke University   (United States)

^e UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOFILMS; BIOREACTORS; CELL CULTURE; CHEMOSTATS; ESCHERICHIA COLI; SYNTHESIS (CHEMICAL);

BIOFILM FORMATION; CELL DENSITY; LONG-TERM CULTURE; MICROFLUIDIC BIOREACTORS;

MICROBIOLOGY;

MICROBIOLOGY;

ARTICLE; BACTERIAL COLONIZATION; BACTERIUM CULTURE; BIOREACTOR; CELL DEATH; CELL DENSITY; CELL STRUCTURE; ESCHERICHIA COLI; FEEDBACK SYSTEM; GENE EXPRESSION; MICROBIAL POPULATION DYNAMICS; MICROFLUIDICS; NONHUMAN; OSCILLATION; PHENOTYPIC VARIATION; PRIORITY JOURNAL; REGULATORY MECHANISM; BACTERIAL COUNT; BACTERIAL GENE; BIOFILM; BIOLOGICAL MODEL; BIOSYNTHESIS; COMPUTER SIMULATION; CULTURE MEDIUM; CYTOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; MATHEMATICS; METABOLISM; MICROBIOLOGICAL EXAMINATION; MUTATION; PHYSIOLOGY; TIME;

ESCHERICHIA COLI;

DRUG DERIVATIVE; GAMMA BUTYROLACTONE; HOMOSERINE LACTONE; ISOPROPYL THIOGALACTOSIDE;

4-BUTYROLACTONE; BACTERIOLOGICAL TECHNIQUES; BIOFILMS; BIOREACTORS; COLONY COUNT, MICROBIAL; COMPUTER SIMULATION; CULTURE MEDIA; ESCHERICHIA COLI; FEEDBACK, BIOCHEMICAL; GENES, BACTERIAL; ISOPROPYL THIOGALACTOSIDE; MATHEMATICS; MICROFLUIDICS; MODELS, BIOLOGICAL; MUTATION; TIME FACTORS;

EID: 21644441288     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1109173     Document Type: Article

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19. We thank M. Elowitz for MC4100Z1 cells, U. Alon for MG1655 cells, T. Ozdere for generating data for figs. S4 and S5, C. Collins for plasmid pLuxR, M. Bamett for technical assistance, and J. Leadbetter, C. Ward, T. Squires, J. Huang, and E. Kartalov for helpful discussions. Supported in part by the NSF and the Defense Advanced Research Projects Agency (contract no. N66001-02-1-8929).