Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas

Methods in Molecular Biology

Volumn 698, Issue , 2011, Pages 123-140

  Seeberger, Karen L ^b   Eshpeter, Alana ^b   Korbutt, Gregory S ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b NONE

Author keywords

Culture; Differentiation; Isolation; Mesenchymal stem cells; Multipotent mesenchymal stromal cells; Nonendocrine pancreas

Indexed keywords

ALKALINE PHOSPHATASE;

ADIPOGENESIS; ARTICLE; BONE DEVELOPMENT; CELL AGGREGATION; CELL SEPARATION; CRYOPRESERVATION; CULTURE TECHNIQUE; CYTOLOGY; FLOW CYTOMETRY; HUMAN; LIPID METABOLISM; METABOLISM; METHODOLOGY; MULTIPOTENT STEM CELL; PANCREAS; STAINING;

ADIPOGENESIS; ALKALINE PHOSPHATASE; CELL AGGREGATION; CELL CULTURE TECHNIQUES; CELL SEPARATION; CRYOPRESERVATION; FLOW CYTOMETRY; HUMANS; LIPID METABOLISM; MULTIPOTENT STEM CELLS; OSTEOGENESIS; PANCREAS; STAINING AND LABELING;

EID: 79959777923     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-999-4_10     Document Type: Chapter

References (41)

1. Dor, Y., Brown, J., Martinez, O. I., and Melton, D. A. (2004) Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature 429, 41–46.
2. Khalaileh, A., Gonen-Gross, T., Magenheim, J., Nir, T., Porat, S., Salpeter, S., Stolovich-Rain, M., Swisa, A., Weinberg, N., and Dor, Y. (2008) Determinants of pancreatic b-cell regeneration. Diabetes Obes. Metab. 10, 128–135.
3. Wang, R. N., Kloppel, G., and Bouwens, L. (1995) Duct-to islet-cell differentiation and islet growth in the pancreas of duct-ligated adult rats. Diabetologia 38, 1405–1411.
4. D’Alessandro, J. S., Lu, K., Fung, B. P., Colman, A., and Clarke, D. L. (2007) Rapid and efficient in vitro generation of pancreatic islet progenitor cells from nonendocrine epithelial cells in the adult human pancreas. Stem Cells Dev. 16, 75–89.
5. Bonner-Weir, S., Taneja, M., Weir, G. C., Tatarkiewicz, K., Song, K. H., Sharma, A., and O’Neil, J. J. (2000) In vitro cultivation of human islets from expanded ductal tissue. PNAS 97, 7999–8004.
6. Heremans, Y., Van de Casteele, C. M., in’t Veld, P., Gradwohl, G., Serup, P., Madsen, O., Pipeleers, D., and Heimberg, H. (2002) Recapitulation of embryonic neuroendocrine differentiation in adult human pancreatic duct cells expressing neurogenin 3. J. Cell Biol. 159, 303–312.
7. Xu, X., D’Hoker, J., Stange, G., Bonne, S., De Leu, N., Xiao, X., Van de Casteele, M., Mellitzer, G., Ling, Z., Pipeleers, D., Bouwens, L., Scharfmann, R., Gradwohl, G., and Heimberg, H. (2008) Beta cells can be generated from endogenous progenitors in injured adult mouse pancreas. Cell 132, 197–207.
8. Yato, S., Dodge, R., Akashi, T., Omer, A., Sharma, A., Weir, G. C., and Bonner-Weir, S. (2007) Differentiation of affinity-purified human pancreatic duct cells to b-cells. Diabetes 56, 1802–1809.
9. Bonner-Weir, S., Inada, A., Yatoh, S., Li, W., Aye, T., Toschi, E., and Sharma, A. (2008) Transdifferentiation of pancreatic ductal cells to endocrine b-cells. Biochem. Soc. Trans. 36, 353–356.
10. Inada, A., Nienaber, C., Katsuta, H., Fugitani, Y., Levine, J., Morita, R., Sharma, A., and Bonner-Weir, S. (2008) Carbonic anhydrase II-positive pancreatic cells are progenitors for both endocrine and exocrine pancreas after birth. PNAS 105, 19915–19919.
11. Hao, E., Tyrberg, B., Itkin-Ansari, P., Lakey, J. R., Geron, I., Monosov, E. Z., Barcova, M., Mercola, M., and Levine, F. (2006) Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas. Nature Med. 12, 310–316.
12. Rooman, I., Lardon, J., and Bouwens, L. (2002) Gastrin stimulates beta-cell neogenesis and increases islet mass from transdifferenti-ated but not from normal exocrine pancreas tissue. Diabetes 51, 686–690.
13. Baeyens, L., De Breuck, S., Lardon, J., Mfopou, J. K., Rooman, I., and Bouwens, L. (2005) In vitro generation of insulin-producing beta cells from adult exocrine pancreatic cells. Diabetologia 48, 49–57.
14. Baeyens, L., and Boewens, L. (2008) Can b-cells be derived from exocrine pancreas? Diabetes Obes. Metab. 10, 170–178.
15. Lipsett, M. A., Castellarin, M. L., and Rosenberg, L. (2007) Acinar plasticity development of a novel in vitro model to study human acinar-to-duct-to-islet differentiation. Pancreas 34, 452–457.
16. Teitelman, G. (2004) Islet-derived multipotential cells/progenitor cells. Cell Biochem. Biophys. 40, 89–102.
17. Gallo, R., Gambelli, F., Gava, B., Sasdelli, F., Tellone, V., Masini, M., Marchetti, P., Dotta, F., and Sorrentino, V. (2007) Generation and expansion of multipotent mesenchymal progenitor cells from cultured human pancreatic islets. Cell Death. Differ. 14, 1860–1871.
18. Eberhardt, M., Salmon, P., von Mach, M. A., Hengstler, J. G., Brulport, M., Linscheid, P., Seboek, D., Oberholzer, J., Barbero, A., Martin, I., Muller, B., Trono, D., and Zulewski, H. (2006) Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets. Biochem. Biophys. Res. Commun. 345, 1167–1176.
19. Gershengorn, M. C., Hardikar, A. A., Wei, C., Geras-Raaka, E., Marcus-Samuels, B., and Raaka, B. M. (2004) Epithelial-to-mesenchymal transition generates proliferative human islet precursor cells. Science 306, 2261–2264.
20. Todorov, I., Omori, K., Pascual, M., Rawson, J., Nair, I., Valiente, L., Vuong, T., Matsuda, T., Orr, C., Ferreri, K., Smith, C. V., Kandeel, F., and Mullen, Y. (2006) Generation of human islets through expansion and differentiation of non-islet pancreatic cells discarded (pancreatic discard) after islet isolation. Pancreas 32, 130–138.
21. Seeberger, K. L., Dufour, J. M., Shapiro, A. M., Lakey, J. R., Rajotte, R. V., Korbutt, G. S. (2006) Expansion of mesenchymal stem cells from human pancreatic ductal epithelium. Lab. Invest. 86, 141–153.
22. Baertschiger, R. M., Bosco, D., Morel, P., Serre-Beinier, V., Berney, T., Buhler, L. H., and Gonelle-Gispert, C. (2008) Mesenchymal stem cells derived from human exocrine pancreas express transcription factors implicated in beta-cell development. Pancreas 37, 75–83.
23. Horwitz, E. M., Le Blanc, K., Dominici, M., Mueller I., Slaper-Cortenbach, I., Marini F. C., Deans, R. J., Krause, D. S., and Keating, A. (2005) Clarification of the nomenclature for MSC: The international society for cellular therapy position statement. Cytotherapy 7, 393–395.
24. Lee, K. D., Kuo, T. K., Whang-Peng, J., Chung, Y. F., Lin, C. T., Chou, S. H., Chen, J.R., Chen, Y. P., and Lee, O. K. (2004) In vitro hepatic dif ferentiation of human mesenchymal stem cells. Hepatology 40, 1275–1284.
25. Seo, M. J., Suh, S. Y., Bae, Y. C., and Jung, J. S. (2005) Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem. Biophys. Res. Commun. 328, 258–264.
26. Timper, K., Seboek, D., Eberhardt, M., Linscheid, P., Christ-Crain, M., Keller, U., Muller, B., and Zulewski, H. (2006) Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells. Biochem. Biophys. Res. Commun. 341, 1135–1140.
27. Battula, V. L., Bareiss, P. M., Treml, S., Conrad, S., Albert, I., Hojak, S., Abele, H., Schewe, B., Just, L., Skutella, T., and Buhring, H. (2007) Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface friz-zled-9 and SSEA-4 and give rise to multilineage differentiation. Differentiation 75, 279–291.
28. Tang, D. Q., Cao, L. Z., Burkhardt, B. R., Xia, C. Q., Litherland, S. A., Atkinson, M. A., and Yang, L. J. (2004) In vivo and in vitro characterization of insulin-producing cells obtained from murine bone marrow. Diabetes 53, 1721–1732.
29. Oh, S. H., Muzzonigro, T. M., Bae, S. H., LaPlante, J. M., Hatch, H. M., and Petersen, B. E. (2004) Adult bone marrow-derived cells trans-differentiating into insulin-producing cells for the treatment of type I diabetes. Lab. Invest. 84, 607–617.
30. Russ, H. A., Bary, Y., Ravassard, P., and Efrat, S. (2008) In vitro proliferation of cells derived from adult human ß-cells revealed by cell-lineage tracing. Diabetes 57, 1575–1583.
31. Atouf, F., Park, C. H., Pechhold, K., Ta, M., Choi,Y., and Lumelsky, N. L. (2007) No evidence for mouse pancreatic beta-cell epithelial-mesenchymal transition in vitro. Diabetes 56, 699–702.
32. Chase, L. G., Ulloa-Montoya, F., Kidder, B. L., and Verfaillie, C. M. (2007) Islet-derived fibroblast-like cells are not derived via epithelial-mesenchymal transition from Pdx-1 or insulin-positive cells. Diabetes 56, 3–7.
33. Morton, R. A., Geras-Raaka, E., Wilson, L. M., Raaka, B. M., and Gershengorn, M. C. (2007) Endocrine precursor cells from mouse islets are not generated by epithelial-to-mesenchymal transition of mature beta cells. Mol. Cell Endocrinol. 270, 87–93.
34. Kayali, A. G., Flores, L. E., Lopez, A. D., Kutlu, B., Baetge, E., Kitamura, R., Hao, E., Beattie, G. M., and Hayek, A. (2007) Limited capacity of human adult islets expanded in vitro to redifferentiate into insulin-producing beta-cells. Diabetes 56, 703–708.
35. Seeberger, K. L., Eshpeter, A., Rajotte, R. V., and Korbutt, G. S., (2009) Epithelial cells within the human pancreas do not coexpress mesenchymal antigens: epithelial-mesenchymal transition is an artifact of cell culture. Lab. Invest. 89, 110–121.
36. Lakey, J. R. T., Warnock, G. L., Shapiro, A. M. J., Korbutt, G. S., Ao, Z., Kneteman, N. M., and Rajotte, R. V. (1999) Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion. Cell Transplant. 8, 285–292.
37. Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., and Marshak, D. R. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284, 143–147.
38. Pittenger, M. F., and Martin, B. J. (2004) Mesenchymal stem cells and their potential as cardiac therapeutics. Circ. Res. 9, 9–20.
39. Prockop, D. J., Phinney, D. J., and Bunnell, B. A. (2008) Mesenchymal stem cells: methods and protocols. Methods in Molecular Biology 449, Humana Press, Totowa.
40. Baxter, M. A., Wynn, R. F., Jowitt, S. N., Wraith, J. E., Fairbairn, L. J., and Bellantuono, I. (2004) Study of telomere length reveals rapid agi ng of human marrow stromal cel l s fol l owing in vitro expansion. Stem Cells 22, 675–682.
41. McManus, J.F.A., and Mowry R. (1960) Staining Methods Histological and Histochemical. Paul B Hoeber, New York.