Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system

Cell Death and Disease

Volumn 1, Issue 10, 2010, Pages

  Tadokoro, D ^a   Takahama, S ^a   Shimizu, K ^a   Hayashi, S ^a   Endo, Y ^a,b   Sawasaki, T ^a,b  

^a EHIME UNIVERSITY   (Japan)

^b RIKEN YOKOHAMA INSTITUTE   (Japan)

Author keywords

Apoptosis; Caspase; Cell free protein synthesis; Protein kinases; Protein li

Indexed keywords

AMKL PROTEIN; BIOTIN; BONE MORPHOGENETIC PROTEIN RECEPTOR 1B; BUB1 RELATED PROTEIN; CASPASE 3; CSK PROTEIN; FAS ANTIBODY; FOCAL ADHESION KINASE 1; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE 1; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE ACTIVATOR; INTERLEUKIN 1 RECEPTOR ASSOCIATED KINASE 2; LIM KINASE 1; MENKES PROTEIN; MICROTUBULE ASSOCIATED PROTEIN 2 K6; MITOGEN ACTIVATED PROTEIN KINASE 12; NUAK 2 PROTEIN; P21 ACTIVATED KINASE 2; PCTAIRE PROTEIN; PEPTIDE LIBRARY; PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE 1; PKT C PROTEIN; PROTEIN KINASE; PROTEIN KINASE B BETA; PROTEIN TYROSINE KINASE; ROCK 1 PROTEIN; STK 3 PROTEIN; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 1; UNCLASSIFIED DRUG; UNINDEXED DRUG;

APOPTOSIS; ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VITRO STUDY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION; ANIMAL; CHEMISTRY; ENZYME SPECIFICITY; METABOLISM; MOUSE;

ANIMALS; CASPASE 3; CELL-FREE SYSTEM; HUMANS; MICE; PEPTIDE LIBRARY; PROTEIN KINASES; SUBSTRATE SPECIFICITY;

EID: 79958155708     PISSN: None     EISSN: 20414889     Source Type: Journal    
DOI: 10.1038/cddis.2010.65     Document Type: Article

References (36)

1. Puente XS, Sanchez LM, Overall CM, Lopez-Otin C. Human and mouse proteases: a comparative genomic approach. Nat Rev Genet 2003; 4: 544-558. (Pubitemid 36781345)
2. Hunter T. Signaling - 2000 and beyond. Cell 2000; 100: 113-127. (Pubitemid 30046300)
3. Manning G, Whyte DB, Martinez R, Hunter T, Sudarsanam S. The protein kinase complement of the human genome. Science 2002; 298: 1912-1934. (Pubitemid 35425239)
4. Matrisian LM, Hogan BL. Growth factor-regulated proteases and extracellular matrix remodeling during mammalian development. Curr Top Dev Biol 1990; 24: 219-259.
5. Turgeon VL, Houenou LJ. The role of thrombin-like (serine) proteases in the development, plasticity and pathology of the nervous system. Brain Res Brain Res Rev 1997; 25: 85-95. (Pubitemid 27459722)
6. van Kempen LC, de Visser KE, Coussens LM. Inflammation, proteases and cancer. Eur J Cancer 2006; 42: 728-734.
7. Friedl P, Wolf K. Tube travel: the role of proteases in individual and collective cancer cell invasion. Cancer Res 2008; 68: 7247-7249.
8. Abdel-Rahman HM, Kimura T, Hidaka K, Kiso A, Nezami A, Freire E et al. Design of inhibitors against HIV, HTLV-I, and Plasmodium falciparum aspartic proteases. Biol Chem 2004; 385: 1035-1039. (Pubitemid 39600311)
9. Alnemri ES. Mammalian cell death proteases: a family of highly conserved aspartate specific cysteine proteases. J Cell Biochem 1997; 64: 33-42. (Pubitemid 27081793)
10. Hengartner MO. The biochemistry of apoptosis. Nature 2000; 407: 770-776.
11. Mahrus S, Trinidad JC, Barkan DT, Sali A, Burlingame AL, Wells JA. Global sequencing of proteolytic cleavage sites in apoptosis by specific labeling of protein N termini. Cell 2008; 134: 866-876.
12. Dix MM, Simon GM, Cravatt BF. Global mapping of the topography and magnitude of proteolytic events in apoptosis. Cell 2008; 134: 679-691.
13. Righetti PG, Boschetti E. Sherlock Holmes and the proteome - a detective story. FEBS J 2007; 274: 897-905. (Pubitemid 46204410)
14. Sawasaki T, Ogasawara T, Morishita R, Endo Y. A cell-free protein synthesis system for high-throughput proteomics. Proc Natl Acad Sci USA 2002; 99: 14652-14657. (Pubitemid 35334539)
15. Sawasaki T, Morishita R, Gouda MD, Endo Y. Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system. Methods Mol Biol 2007; 375: 95-106. (Pubitemid 350183383)
16. Takai K, Sawasaki T, Endo Y. Practical cell-free protein synthesis system using purified wheat embryos. Nat Protoc 2010; 5: 227-238.
17. Endo Y, Sawasaki T. Cell-free expression systems for eukaryotic protein production. Curr Opin Biotechnol 2006; 17: 373-380. (Pubitemid 44163458)
18. Sawasaki T, Hasegawa Y, Morishita R, Seki M, Shinozaki K, Endo Y. Genome-scale, biochemical annotation method based on the wheat germ cell-free protein synthesis system. Phytochemistry 2004; 65: 1549-1555. (Pubitemid 38969936)
19. Goshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R et al. Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nat Methods 2008; 5: 1011-1017.
20. Sawasaki T, Kamura N, Matsunaga S, Saeki M, Tsuchimochi M, Morishita R et al. Arabidopsis HY5 protein functions as a DNA-binding tag for purification and functional immobilization of proteins on agarose/DNA microplate. FEBS Lett 2008; 582: 221-228.
21. Takahashi H, Nozawa A, Seki M, Shinozaki K, Endo Y, Sawasaki T. A simple and highsensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis. BMC Plant Biol 2009; 9: 39.
22. Matsuoka K, Komori H, Nose M, Endo Y, Sawasaki T. Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis. J Proteome Res 2010; 9: 4264-4273.
23. Fischer U, Janicke RU, Schulze-Osthoff K. Many cuts to ruin: a comprehensive update of caspase substrates. Cell Death Differ 2003; 10: 76-100. (Pubitemid 36511834)
24. Kurokawa M, Kornbluth S. Caspases and kinases in a death grip. Cell 2009; 138: 838-854.
25. Lee KK, Murakawa M, Nishida E, Tsubuki S, Kawashima S, Sakamaki K et al. Proteolytic activation of MST/Krs, STE20-related protein kinase, by caspase during apoptosis. Oncogene 1998; 16: 3029-3037. (Pubitemid 28297725)
26. Tomiyoshi G, Horita Y, Nishita M, Ohashi K, Mizuno K. Caspase-mediated cleavage and activation of LIM-kinase 1 and its role in apoptotic membrane blebbing. Genes Cells 2004; 9: 591-600. (Pubitemid 38877787)
27. Tatsuta T, Shiraishi A, Mountz JD. The prodomain of caspase-1 enhances Fas-mediated apoptosis through facilitation of caspase-8 activation. J Biol Chem 2000; 275: 14248-14254. (Pubitemid 30339704)
28. Packard BZ, Komoriya A, Brotz TM, Henkart PA. Caspase 8 activity in membrane blebs after anti-Fas ligation. J Immunol 2001; 167: 5061-5066. (Pubitemid 33009739)
29. Lahti JM, Xiang J, Heath LS, Campana D, Kidd VJ. PITSLRE protein kinase activity is associated with apoptosis. Mol Cell Biol 1995; 15: 1-11. (Pubitemid 24379894)
30. Emoto Y, Manome Y, Meinhardt G, Kisaki H, Kharbanda S, Robertson M et al. Proteolytic activation of protein kinase C delta by an ICE-like protease in apoptotic cells. EMBO J 1995; 14: 6148-6156. (Pubitemid 26007576)
31. Casciola-Rosen LA, Anhalt GJ, Rosen A. DNA-dependent protein kinase is one of a subset of autoantigens specifically cleaved early during apoptosis. J Exp Med 1995; 182: 1625-1634.
32. Pearce LR, Komander D, Alessi DR. The nuts and bolts of AGC protein kinases. Nat Rev Mol Cell Biol 2010; 11: 9-22.
33. Ohoka N, Sakai S, Onozaki K, Nakanishi M, Hayashi H. Anaphase-promoting complex/ cyclosome-cdh1 mediates the ubiquitination and degradation of TRB3. Biochem Biophys Res Commun 2010; 392: 289-294.
34. Sawasaki T, Hasegawa Y, Tsuchimochi M, Kamura N, Ogasawara T, Kuroita T et al. A bilayer cell-free protein synthesis system for high-throughput screening of gene products. FEBS Lett 2002; 514: 102-105. (Pubitemid 34251251)
35. Kamura N, Sawasaki T, Kasahara Y, Takai K, Endo Y. Selection of 50-untranslated sequences that enhance initiation of translation in a cell-free protein synthesis system from wheat embryos. Bioorg Med Chem Lett 2005; 15: 5402-5406. (Pubitemid 41562958)
36. Crooks GE, Hon G, Chandonia JM, Brenner SE. WebLogo: a sequence logo generator. Genome Res 2004; 14: 1188-1190. (Pubitemid 38811555)