Monitoring of gene expression in bacteria during infections using an adaptable set of bioluminescent, fluorescent and colorigenic fusion vectors

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Uliczka, Frank ^a,b   Pisano, Fabio ^a   Kochut, Annika ^a   Opitz, Wiebke ^a   Herbst, Katharina ^a   Stolz, Tatjana ^a   Dersch, Petra ^a,b  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; CHLORAMPHENICOL; KANAMYCIN; TETRACYCLINE; PHOTOPROTEIN;

AMCYAN GENE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL INFECTION; BIOLUMINESCENCE; COLORIMETRY; CONTROLLED STUDY; DSRED2 GENE; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; FLUORESCENCE; FUSION GENE; GAPA GENE; GENE CASSETTE; GENE CONSTRUCT; GENE DOSAGE; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; GFP GENE; HUMAN; HUMAN CELL; LACZ GENE; LUXCDABE GENE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROMOTER REGION; REPLICON; REPORTER GENE; RNA TRANSLATION; ANIMAL; BACTERIUM; CELL LINE; GENE EXPRESSION REGULATION; GENE FUSION; GENE VECTOR; GENETICS; PATHOGENICITY; YERSINIA PSEUDOTUBERCULOSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; YERSINIA PSEUDOTUBERCULOSIS;

ANIMALS; ARTIFICIAL GENE FUSION; BACTERIA; BACTERIAL INFECTIONS; BASE SEQUENCE; CELL LINE; FEMALE; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; LUMINESCENT PROTEINS; MICE; PLASMIDS; PROMOTER REGIONS, GENETIC; YERSINIA PSEUDOTUBERCULOSIS;

EID: 79958034631     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020425     Document Type: Article

References (38)

1. Jakobs S, Subramaniam V, Schonle A, Jovin TM, Hell SW, (2000) EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy. FEBS Lett 479: 131-135.
2. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC, (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
3. Viallet PM, Vo-Dinh T, (2003) Monitoring intracellular proteins using fluorescence techniques: from protein synthesis and localization to activity. Curr Protein Pept Sci 4: 375-388.
4. Pakhomov AA, Martynov VI, (2008) GFP family: structural insights into spectral tuning. Chem Biol 15: 755-764.
5. Yang F, Moss LG, Phillips GN Jr, (1996) The molecular structure of green fluorescent protein. Nat Biotechnol 14: 1246-1251.
6. Cormack BP, Valdivia RH, Falkow S, (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
7. Crameri A, Whitehorn EA, Tate E, Stemmer WP, (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol 14: 315-319.
8. Sample V, Newman RH, Zhang J, (2009) The structure and function of fluorescent proteins. Chem Soc Rev 38: 2852-2864.
9. Bevis BJ, Glick BS, (2002) Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat Biotechnol 20: 83-87.
10. Baird GS, Zacharias DA, Tsien RY, (2000) Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A 97: 11984-11989.
11. Chudakov DM, Matz MV, Lukyanov S, Lukyanov KA, (2010) Fluorescent proteins and their applications in imaging living cells and tissues. Physiol Rev 90: 1103-1163.
12. Goulding A, Shrestha S, Dria K, Hunt E, Deo SK, (2008) Red fluorescent protein variants with incorporated non-natural amino acid analogues. Protein Eng Des Sel 21: 101-106.
13. Greer LF 3rd, Szalay AA, (2002) Imaging of light emission from the expression of luciferases in living cells and organisms: a review. Luminescence 17: 43-74.
14. Hutchens M, Luker GD, (2007) Applications of bioluminescence imaging to the study of infectious diseases. Cell Microbiol 9: 2315-2322.
15. Hughes KT, Maloy SR, (2007) Use of operon and gene fusions to study gene regulation in Salmonella. Methods Enzymol 421: 140-158.
16. Silhavy TJ, Beckwith JR, (1985) Uses of lac fusions for the study of biological problems. Microbiol Rev 49: 398-418.
17. Silhavy TJ, Berman ML, Enquist LW, (1984) Experiments with gene fusions: Cold Spring Harbor Laboratory Press.
18. Matthysse AG, Stretton S, Dandie C, McClure NC, Goodman AE, (1996) Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli. FEMS Microbiol Lett 145: 87-94.
19. Karunakaran R, Mauchline TH, Hosie AH, Poole PS, (2005) A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in Gram-negative bacteria. Microbiology 151: 3249-3256.
20. Sambrook J, Russell DW, (2001) Molecular cloning: a laboratory manual.
21. Lutz R, Bujard H, (1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res 25: 1203-1210.
22. Nagel G, Lahrz A, Dersch P, (2001) Environmental control of invasin expression in Yersinia pseudotuberculosis is mediated by regulation of RovA, a transcriptional activator of the SlyA/Hor family. Mol Microbiol 41: 1249-1269.
23. Strauch E, Voigt I, Broll H, Appel B, (2000) Use of a plasmid of a Yersinia enterocolitica biogroup 1A strain for the construction of cloning vectors. J Biotechnol 79: 63-72.
24. Miller VL, Mekalanos JJ, (1988) A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane protein virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 170: 2575-2583.
25. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, et al. (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 17: 969-973.
26. Labas YA, Gurskaya NG, Yanushevich YG, Fradkov AF, Lukyanov KA, et al. (2002) Diversity and evolution of the green fluorescent protein family. Proc Natl Acad Sci U S A 99: 4256-4261.
27. Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and evolution of coral fluorescent proteins. PLoS One 3: e2680.
28. Metcalf WW, Wanner BL, (1993) Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129: 17-25.
29. El Tahir Y, Skurnik M, (2001) YadA, the multifaceted Yersinia adhesin. Int J Med Microbiol 291: 209-218.
30. Skurnik M, Toivanen P, (1992) LcrF is the temperature-regulated activator of the yadA gene of Yersinia enterocolitica and Yersinia pseudotuberculosis. J Bacteriol 174: 2047-2051.
31. Hakkila K, Maksimow M, Karp M, Virta M, (2002) Reporter genes lucFF, luxCDABE, gfp, and dsred have different characteristics in whole-cell bacterial sensors. Anal Biochem 301: 235-242.
32. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, et al. (1998) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 64: 2240-2246.
33. Miller WG, Leveau JH, Lindow SE, (2000) Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol Plant Microbe Interact 13: 1243-1250.
34. Herbst K, Bujara M, Heroven AK, Opitz W, Weichert M, et al. (2009) Intrinsic thermal sensing controls proteolysis of Yersinia virulence regulator RovA. PLoS Pathog 5: e1000435.
35. Pettersson J, Nordfelth R, Dubinina E, Bergman T, Gustafsson M, et al. (1996) Modulation of virulence factor expression by pathogen target cell contact. Science 273: 1231-1233.
36. Valdivia RH, Falkow S, (1997) Probing bacterial gene expression within host cells. Trends Microbiol 5: 360-363.
37. Trcek J, Berschl K, Trulzsch K, (2010) In vivo analysis of Yersinia enterocolitica infection using luxCDABE. FEMS Microbiol Lett 307: 201-206.
38. Karsi A, Menanteau-Ledouble S, Lawrence ML, (2006) Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring. FEMS Microbiol Lett 260: 216-223.