Interfertile oaks in an island environment: I. High nuclear genetic differentiation and high degree of chloroplast DNA sharing between Q. alnifolia and Q. coccifera in Cyprus. A multipopulation study

European Journal of Forest Research

Volumn 130, Issue 4, 2011, Pages 543-555

  Neophytou, Charalambos ^a,b   Dounavi, Aikaterini ^a   Fink, Siegfried ^b   Aravanopoulos, Filippos A ^c  

^a Forest Research Institute of Baden Wurttemberg   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

cpDNA haplotypes; DNA microsatellites; Genetic differentiation; Genetic introgression; Genetic structure; Hybridization; Quercus alnifolia; Quercus coccifera; Spatial genetic barriers

Indexed keywords

ANGIOSPERM; BAYESIAN ANALYSIS; CHLOROPLAST; DNA; GENETIC ANALYSIS; GENETIC DIFFERENTIATION; GENETIC VARIATION; GEOGRAPHICAL DISTRIBUTION; GROWTH; HYBRIDIZATION; INTROGRESSION;

CYPRUS;

QUERCUS; QUERCUS COCCIFERA;

EID: 79957922204     PISSN: 16124669     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10342-010-0442-8     Document Type: Article

References (61)

1. Barbero M, Quezel P (1979) Contribution à l'étude des groupements forestiers de Chypre. Documents Phytosociologiques IV: 9-34.
2. Belahbib N, Pemonge M, Ouassou A, Sbay H, Kremer A, Petit RJ (2001) Frequent cytoplasmic exchanges between oak species that are not closely related: Quercus suber and Q. ilex in Morocco. Mol Ecol 10: 2003-2012.
3. Boavida LC, Silva JP, Feijó JA (2001) Sexual reproduction in the cork oak (Quercus suber L). II. Crossing intra- and interspecific barriers. Sex Plant Reprod 14: 143-152.
4. Burgarella C, Lorenzo Z, Jabbour-Zahab R, Lumaret R, Guichoux E, Petit RJ, Soto A, Gil L (2009) Detection of hybrids in nature: application to oaks (Quercus suber and Q. ilex). Heredity 102: 442-452.
5. Butzer KW, Harris SE (2007) Geoarchaeological approaches to the environmental history of Cyprus: explication and critical evaluation. J Archaeol Sci 34: 1932-1952.
6. Christou A (2001) Cyprus (country report). Mediterranean Oaks Network: Report of the first meeting: 12-14 October 2000-Antalya, Turkey 3.
7. Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9: 1657-1659.
8. Craft KJ, Ashley MV (2006) Population differentiation among three species of white oak in northeastern Illinois. Can J Forest Res 36: 206-215.
9. Curtu AL, Gailing O, Leinemann L, Finkeldey R (2007a) Genetic variation and differentiation within a natural community of five oak species (Quercus spp.). Plant Biol 9: 116-126.
10. Curtu AL, Gailing O, Finkeldey R (2007b) Evidence for hybridization and introgression within a species-rich oak (Quercus spp.) community. BMC Evol Biol 7: 218-233.
11. Darwin C (1859) On the origin of species by natural selection. Murray, London.
12. Deguilloux M, Dumolin-Lapègue S, Gielly L, Grivet D, Petit RJ (2003) A set of primers for the amplification of chloroplast microsatellites in Quercus. Mol Ecol Notes 3: 24-27.
13. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14: 2611-2620.
14. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 479-491.
15. Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3. 0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1: 47-50.
16. Fineschi S, Cozzolino S, Migliaccio M, Musacchio A, Innocenti M, Vendramin GG (2005) Sicily represents the Italian reservoir of chloroplast DNA diversity of Quercus ilex L. (Fagaceae). Ann For Sci 62: 79-84.
17. Goudet J (1995) FSTAT (version 1. 2): A computer program to calculate F-statistics. J Hered 86: 485-486.
18. Gugerli F, Walser J, Dounavi K, Holderegger R, Finkeldey R (2007) Coincidence of small-scale spatial discontinuities in leaf morphology and nuclear microsatellite variation of Quercus petraea and Q. robur in a mixed forest. Ann Bot 99: 713-722.
19. Gugerli F, Brodbeck S, Holderegger R (2008) Utility of multilocus genotypes for taxon assignment in stands of closely related European white oaks from Switzerland. Ann Bot 102: 855-863.
20. Hand R (2006) Supplementary notes to the flora of Cyprus V. Willdenowia 36: 761-809.
21. Jiménez P, López de Heredia U, Collada C, Lorenzo Z, Gil L (2004) High variability of chloroplast DNA in three Mediterranean evergreen oaks indicates complex evolutionary history. Heredity 93: 510-515.
22. n Microsatellite Loci from Quercus robur. Hereditas 129: 183-186.
23. Knopf H (2006) Quercus alnifolia Poech. In: Schütt P, Schuck HJ, Lang UM, Roloff A (eds). Enzyklopädie der Holzgewächse 3. Landsberg am Lech, pp 1-7.
24. Kremer A, Kleinschmit J, Cottrell J et al (2002) Is there a correlation between chloroplastic and nuclear divergence, or what are the roles of history and selection on genetic diversity in European oaks? For Ecol Manage 156: 75-87.
25. Lexer C, Kremer A, Petit RJ (2006) Shared alleles in sympatric oaks: recurrent gene flow is a more parsimonious explanation than ancestral polymorphism. Mol Ecol 15: 2007-2012.
26. López de Heredia U, Jiménez P, Díaz-Fernández P, Gil L (2005) The Balearic Islands: a reservoir of cpDNA genetic variation for evergreen oaks. J Biogeogr 32: 939-949.
27. López de Heredia U, Carrión JS, Jiménez P, Collada C, Gil L (2007a) Molecular and palaeoecological evidence for multiple glacial refugia for evergreen oaks on the Iberian Peninsula. J Biogeogr 34: 1505-1517.
28. López de Heredia U, Jiménez P, Collada C, Simeone MC, Bellarosa R, Schirone B, Cervera MT, Gil L (2007b) Multi-marker phylogeny of three evergreen oaks reveals vicariant patterns in the Western Mediterranean. Taxon 56: 1209-1220.
29. Lorenzo Z, Burgarella C, de Heredia UL, Lumaret R, Petit RJ, Soto A, Gil L (2009) Relevance of genetics for conservation policies: the case of Minorcan cork oaks. Ann Bot 104: 1069-1076.
30. Lumaret R, Jabbour-Zahab R (2009) Ancient and current gene flow between two distantly related Mediterranean oak species, Quercus suber and Q. ilex. Ann Bot 104: 725-736.
31. Lumaret R, Mir C, Michaud H, Raynal V (2002) Phylogeographical variation of chloroplast DNA in holm oak (Quercus ilex L.). Mol Ecol 11: 2327-2336.
32. Magri D, Fineschi S, Bellarosa R, Buonamici A, Sebastiani F, Schirone B, Simeone MC, Vnedramin GG (2007) The distribution of Quercus suber chloroplast haplotypes matches the palaeogeographical history of the western Mediterranean. Mol Ecol 16: 5259-5266.
33. Manni F, Guérard E, Heyer E (2004) Geographic patterns of (genetic, morphologic, linguistic) variation. Hum Biol 76: 173-190.
34. Michalakis Y, Excoffier L (1996) A generic estimation of population subdivision using distances between alleles with special reference for microsatellite loci. Genetics 142: 1061.
35. Minch E, Ruiz-Linares A, Goldstein D, Feldman M, Cavalli-Sforza LL (1995) MICROSAT, The microsatellite distance program. http://human. stanford. edu/ Stanford California: Stanford University.
36. Mir C, Jarne P, Sarda V, Bonin A, Lumaret R (2009) Contrasting nuclear and cytoplasmic exchanges between phylogenetically distant oak species (Quercus suber L. and Q. ilex L.) in Southern France: inferring crosses and dynamics. Plant Biol 11: 213-226.
37. Monmonier M (1973) Maximum-difference barriers: An alternative numerical regionalization method. Geogr Anal 3: 245-261.
38. Muir G, Schlötterer C (2005) Evidence for shared ancestral polymorphism rather than recurrent gene flow at microsatellite loci differentiating two hybridizing oaks (Quercus spp.). Mol Ecol 14: 549-561.
39. Muir G, Fleming CC, Schlotterer C (2000) Species status of hybridizing oaks. Nature 405: 1016.
40. Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
41. Neophytou C, Palli G, Dounavi A, Aravanopoulos F (2007) Morphological differentiation and hybridization between Quercus alnifolia Poech and Quercus coccifera L. (Fagaceae) in Cyprus. Silvae Genet 56: 271-277.
42. Neophytou C, Dounavi A, Aravanopoulos F (2008) Conservation of nuclear SSR loci reveals high affinity of Quercus infectoria ssp. veneris A. Kern (Fagaceae) to section Robur. Plant Mol Biol Rep 26: 133-141.
43. Nosil P, Funk DJ, Ortiz-Barrientos D (2009) Divergent selection and heterogeneous genomic divergence. Mol Ecol 18: 375-402.
44. Palamarev E (1989) Paleobotanical evidences of the Tertiary history and origin of the Mediterranean sclerophyll dendroflora. Plant Syst Evol 162: 93-107.
45. Peakall R, Smouse PE (2006) GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6: 288-295.
46. Petit RJ, El Mousadik A, Pons O (1998) Identifying populations for conservation on the basis of genetic markers. Conserv Biol 12: 844-855.
47. Petit RJ, Csaikl UM, Bordács S et al (2002) Chloroplast DNA variation in European white oaks: Phylogeography and patterns of diversity based on data from over 2600 populations. For Ecol Manage 156: 5-26.
48. Petit RJ, Bodenes C, Ducousso A, Roussel G, Kremer A (2004) Hybridization as a mechanism of invasion in oaks. New Phytol 161: 151-164.
49. Petit RJ, Duminil J, Fineschi S, Hampe A, Salvini D, Vendramin GG (2005) Comparative organization of chloroplast, mitochondrial and nuclear diversity in plant populations. Mol Ecol 14: 689-701.
50. Pons O, Petit RJ (1995) Estimation, variance and optimal sampling of gene diversity. Theor Appl Genet 90: 462-470.
51. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945-959.
52. Rubio de Casas R, Cano E, Balaguer L, Perez-Corona E, Manrique E, García-Verdugo C, Vargas P (2007) Taxonomic identity of Quercus coccifera L. in the Iberian Peninsula is maintained in spite of widespread hybridisation, as revealed by morphological, ISSR and ITS sequence data. Flora 202: 488-499.
53. Salvini D, Bruschi P, Fineschi S, Grossoni P, Kjær ED, Vendramin GG (2009) Natural hybridisation between Quercus petraea (Matt.) Liebl. and Quercus pubescens Willd. within an Italian stand as revealed by microsatellite fingerprinting. Plant Biol 11: 758-765.
54. Scotti-Saintagne C, Bodénès C, Barreneche T, Bertocchi E, Plomion C, Kremer A (2004) Genome scanning for interspecific differentiation between two closely related oak species [Quercus robur L. and Q. petraea (Matt.) Liebl.]. Genetics 168: 1615-1626.
55. Slatkin M (1995) A measure of population subdivision based on microsatellite allele frequencies. Genetics 139: 457-462.
56. Soto A, Lorenzo Z, Gil L (2007) Differences in fine-scale genetic structure and dispersal in Quercus ilex L. and Q. suber L.: consequences for regeneration of mediterranean open woods. Heredity 99: 601-607.
57. n- microsatellite loci from Quercus petraea. Plant Mol Biol 33: 1093-1096.
58. Toumi L, Lumaret R (2001) Allozyme characterisation of four Mediterranean evergreen oak species. Biochem Syst Ecol 29: 799-817.
59. van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4: 535-538.
60. van Oosterhout C, Weetman D, Hutchinson WF (2006) Estimation and adjustment of microsatellite null alleles in nonequilibrium populations. Mol Ecol Notes 6: 255-256.
61. Weising K, Gardner RC (1999) A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms. Genome 42: 9-19.