Investigation of cytotoxic, apoptosis-inducing, genotoxic and protective effects of the flavonoid rutin in HTC hepatic cells

Experimental and Toxicologic Pathology

Volumn 63, Issue 5, 2011, Pages 459-465

  Cristina Marcarini, Juliana ^a   Ferreira Tsuboy, Marcela Stefanini ^a   Cabral Luiz, Rodrigo ^a   Regina Ribeiro, Lucia ^b   Beatriz Hoffmann Campo, Clara ^c   Ségio Mantovani, Mário ^a  

^a STATE UNIVERSITY OF LONDRINA   (Brazil)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^c EMBRAPA CERRADOS   (Brazil)

Author keywords

Apoptosis; Cytotoxicity; Flavonoid rutin; HTC; Protector effect

Indexed keywords

BENZO[A]PYRENE; CISPLATIN; DOXORUBICIN; RUTOSIDE; TETRAZOLIUM;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL GROWTH; CELL PROLIFERATION; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; CYTOKINESIS; DNA DAMAGE; DRUG CYTOTOXICITY; GEL ELECTROPHORESIS; GENOTOXICITY; LIVER CELL; LIVER PROTECTION; MICRONUCLEUS; NONHUMAN; RAT;

ANIMALS; ANTIMUTAGENIC AGENTS; APOPTOSIS; BENZO(A)PYRENE; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; COMET ASSAY; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; LIVER NEOPLASMS, EXPERIMENTAL; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MUTAGENS; RATS; RUTIN;

EID: 79955958784     PISSN: 09402993     EISSN: 16181433     Source Type: Journal    
DOI: 10.1016/j.etp.2010.03.005     Document Type: Article

References (41)

1. Afanas' ev IB.Oxidative stress in rheumathoid arthritis leukocytes: supression by rutin and other antioxidants and chelators. Biochem Pharm 2001, 62:743-746.
2. Autore G., Rastrelli L., Lauro M.L., Marzocc P., Sorrentino M., Sorrentino U., et al. Inhibition of nitric oxide synthase expression by a methanolic extract of Crescentia alata and its derived flavonols. Life Sciences 2001, 70:523-534.
3. Bagchi M., Bagchi S.J., Stohs S.D., Ray C.K., Sen H.G. Cellular protection with proanthocyanidins derived from grape seeds. Ann N Y Acad Sci 2002, 957:260-270.
4. Birt D.F., Hendrich P., Weiqun W. Dietary agents in cancer prevention: flavonoids and isoflavonoids. Plant Foods Hum Nut 2001, 48:112-120.
5. Das A., Wang J.H., Lien E.J. Carcinogenicity, mutagenicity and cancer preventing activities of flavonoids: a structure-system-activity relationship (SSAR) analysis. Prog Drug Res 1994, 42:133-167.
6. Deschner J., Ruperto G., Wong H.L. Quercetin and rutin as inhibitors of azoxymethanol-induced colonic neoplasia. Carcinogenesis 1991, 12:1193-1196. D.
7. Doonan F., Cotter T.G. Morphological assessment of apoptosis. Methods 2008, 44:200-204.
8. Fenech M. The in vitro micronucleus technique. Mut Res 2000, 455:81-95.
9. Flores J., González-Gallego J.M., Culebras P. Los flavonoides: propiedades y acciones antioxidantes. Nutr Hos 2002, 17:271-278.
10. Harborne J.B., Williams C.A. Advances in flavonoid research since 1992. Phytochemistry 2000, 55:481-504.
11. Hirano R., Tokorozawa S., Sasamoto W., Matsumoto A., Itakura H., Igarashi O., et al. Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. J Nut Sci Vitam 2001, 475:57-362.
12. Hollman P.C.H., Katan M.B. Analysis and health effects of flavonoids. Food Chem 1996, 57:43-46.
13. Hyun-Jung K., Hyang-Sook C., Yang R. Inhibition of benzo[a]pyrene-induced cytotoxicity and cytochrome P4501A activity by dietary flavonoids in human liver cell model: structure-activity relationship. Biotech Lett 2000, 22:1941-1946.
14. Iffert T., Soldan M., Moeller A., Maser E. Modulation of daunorubicin toxicity by liposomal encapsulation and use of specific inhibitors in vitro. Toxicology 2000, 144:189-195.
15. Janbaz A., Sheikh A., Saeedb A.H. Protective effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents. Fitoterapia 2002, 73:557-563.
16. 2-induced apoptosis via anti-oxidant. Biochem Pharm 2005, 6:1839-1851.
17. Kennedy D.O., Kojima A., Moffati J. Cellular thiol status-dependent inhibition of tumor cell growth via modulation of retiniblastoma protein phosphorylation by (-)- epigallocatechin. Cancer Lett 2002, 179:25-32.
18. Kim G., Jang H. Protective Mechanism of Quercetin and Rutin Using Glutathione Metabolism on H2O2-induced Oxidative Stress in HepG2 Cells. Ann NY Acad Sci 2009, 1171:1530-1537.
19. Kobayash H., Sugiyama C., Morikawa Y., Hayashi M., Sofuni T. A comparison between manual microscopic analysis and computerized image analysis in the single cell gel electrophoresis assay. MMS Commun 1995, 3:103-115.
20. Lee C.S., Park S.Y., Ko H.H., Han E.S. Effect of change in cellular GSH levels on mitochondrial damage and cell viability loss due to mitomycin c in small cell lung cancer cells. Biochem Pharm 2004, 68:1857-1867.
21. Lima L.R.P., Oliveira T.T., Oliveira M.G.A., Nagem T.J., Pinto A.S.P., Gome S.M. Determinação da atividade de lipase na presença de Morina, Naringenina, Naringina e Rutina. Cienc Agrotec Lavras 1999, 23:626-631.
22. Matsui J., Kiyokawa U., Takenouchi T., Taguchi T., Suzuki K., Shiozawa M., et al. Dietary bioflavonoids induce apoptosis in human leukemia cells. Leuk Res 2005, 29:573-581.
23. Mishell B.B., Shiiqi S.M., Henry C., Chen E.L., North J.R., Gallily M., et al. Apoptosis: quantitative analysis techniques. Sel Methods Cell Immunol 1980, 21-22.
24. Mosmann T. Rapid Colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65:55-56.
25. Nijveldt R.J., Nood E.V., DECV Hoorn, Boelens P.G., Norren K.V. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 2001, 4:418-425.
26. Oliveira J.M., Jordão B.Q., Ribeiro L.R., Ferreira D.A., Eira A., Mantovani M.S. Anti-genotoxic effect of aqueous extracts of sun mushroom (Agaricus blazei Murril lineage 99/26) in mammalian cells in vitro. Food Chem Toxicol 2002, 40:1775-1780.
27. Pathak D., Pathak K., Singla A.K. Flavonoids as medicinal agents: recent advances. Fitoterapia 1991, 57:371-389.
28. Romero A., Pa'ez A., Ferruelo M., Luja N., Berenguer A. Polyphenols in red wine inhibit the proliferation and induce apoptosis of LNCaP cells. BJU Int 2002, 89:950-954.
29. Rovozzo G.C., Burke C.N. A Manual of Basic Virological Techniques 1973, Prentice Hall, New Jersey.
30. Ruiz E., Padilla E., Redondo S., Gordillo-Moscoso A. Kaempferol inhibits apoptosis in vascular smooth muscle induced by a component of oxidized LDL. Eur J Pharm 2006, 529:79-83.
31. Silva J., Hermann S.M., Heuser W., Marroni N., González-Gallego J., Erdtmann B. Evaluation of the genotoxic effect of rutin and quercetin by comet assay and micronucleus test. Food Chem Toxicol 2002, 40:941-947.
32. Soares E.A., Varanda B., MSG, Raddi B. In vitro basal and metabolism-mediated cytotoxicity of flavonoids. Food Chem Toxicol 2006, 44:835-838.
33. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., et al. Single Cell Gel/Comet assay: Guideline for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagenesis 2000, 35:206-221.
34. Tsuboy M.S., Marcarini J.C., Luiz R.C., Barros I.B., Ferreira D.T., Ribeiro L.R., et al. In Vitro evaluation of the genotoxic activity and apoptosis induction of the extracts of roots and leaves from the medicinal plant coccoloba mollis (Polygonaceae). J Med Food 2010, 13:1-6. DOI: 10.1089.
35. Waters M.D., Brady A.L., Stack H.F., Brockman H.E. Antimutagenic profiles for some model compounds. Mutat Res 1990, 238:57-85.
36. Wyllie A.H. Apoptosis, cell death and cell proliferation. Roche Appl Sci Man 2008.
37. Woo K., Myung H., Banga E.S., Kima E., Kyeong C. Quercetin decreases the expression of ErbB2 and ErbB3 proteinsin HT-29 human colon cancer cellsB. J Nutr Biochem 2005, 6:155-162.
38. Woo K., Jeong R.B., Hawes C.M. Flavonoids: from cell cycle regulation to biotechnology. R Biotech Lett 2005, 27:365-374.
39. Yao L.H., Jiang Y.M., Shi J., Tomás-Barberán F.A., Datta N., Singanusong R., et al. Flavonoids in food and their health benefits. Plant Foods Hum Nutr 2004, 59:113-122.
40. Youdim K.A. Potencial role of dietary flavonoids in reducing microvascular endothelium vulnerability to oxidative and inflammatory insults. J Nutr Biochem 2002, 13:282-288.
41. Yu C.B., Swaminathan B., Butler L.G., Pratt D.E. Isolation and identification of rutin as the major mutagenic of red wine. Mutat Res 1986, 170:103-113.