A novel and simple PCR walking method for rapid acquisition of long DNA sequence flanking a known site in microbial genome

Molecular Biotechnology

Volumn 47, Issue 3, 2011, Pages 220-228

  Luo, Peng ^a   Su, Ting ^a   Hu, Chaoqun ^a   Ren, Chunhua ^a  

^a SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY   (China)

Author keywords

Flanking sequence; Long PCR; Microbial genome; Vibrio alginolyticus; Vibrio cholerae; Vibrio vulnificus; Walking

Indexed keywords

BACTERIA; CLONING; DNA; ELECTROPHORESIS; GENES; GENETIC ENGINEERING; PURIFICATION;

FLANKING SEQUENCE; LONG PCR; MICROBIAL GENOMES; VIBRIO ALGINOLYTICUS; VIBRIO CHOLERAE; VIBRIO VULNIFICUS; WALKING;

POLYMERASE CHAIN REACTION;

ARTICLE; DNA FLANKING REGION; ELECTROPHORESIS; GENE AMPLIFICATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; VIBRIO ALGINOLYTICUS; VIBRIO CHOLERAE; VIBRIO VULNIFICUS; BACTERIAL GENOME; GENETICS; METHODOLOGY;

VIBRIO ALGINOLYTICUS; VIBRIO CHOLERAE; VIBRIO VULNIFICUS;

BASE SEQUENCE; GENOME, BACTERIAL; POLYMERASE CHAIN REACTION; VIBRIO CHOLERAE;

EID: 79955850039     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9332-z     Document Type: Article

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