Sequence analysis of TnphoA insertion sites in Vibro cholerae mutants defective in rugose polysaccharide production

Infection and Immunity

Volumn 68, Issue 12, 2000, Pages 6857-6864

  Ali, Afsar ^a   Mahmud, Zahid Hayat ^a   Morris Jr , J Glenn ^a   Sozhamannan, Shanmuga ^a   Johnson, Judith A ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL POLYSACCHARIDE;

ARTICLE; BACTERIUM ISOLATION; CHOLERA; GENE LOCUS; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; VIBRIO CHOLERAE;

ALKALINE PHOSPHATASE; CHROMOSOME MAPPING; CYCLIN-DEPENDENT KINASES; DNA TRANSPOSABLE ELEMENTS; MUTATION; POLYMERASE CHAIN REACTION; POLYSACCHARIDES, BACTERIAL; VIBRIO CHOLERAE;

EID: 0034440780     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.12.6857-6864.2000     Document Type: Article

References (42)

1. Ali, A., J. A. Johnson, A. A. Franco, D. J. Metzger, T. D. Connell, J. G. Morris, Jr., and S. Sozhamannan. 2000. Mutations in the extracellular protein secretion pathway genes (eps) interfere with rugose polysaccharide production in and motility of Vibrio cholerae. Infect. Immun. 68:1967-1974.
2. Cameron, D. N., F. M. Khambaty, I. K. Wachsmuth, R. V. Tauxe, and T. J. Barrett. 1994. Molecular characterization of Vibrio cholerae O1 strains by pulsed-field gel electrophoresis. J. Clin. Microbiol. 32:1685-1690.
3. Chiang, S. L., and J. J. Mekalanos. 1998. Use of signature tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol. Microbiol. 27:797-805.
4. Colwell, R. R., P. R. Brayton, D. J. Grimes, D. B. Roszak, A. Huq, and L. M. Palmer. 1985. Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Bio/Technology 3:817-820.
5. Colwell, R. R., and A. Huq. 1994. Vibrios in the environment: viable but nonculturable Vibrio cholerae, p. 117-133. In I. K. Wachsmuth, P. A. Blake, and O. Olsvik (ed.), Vibrio cholerae and cholera: molecular to global perspectives. ASM Press, Washington, D.C.
6. Donnenberg, M. S., and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive selection suicide vector. Infect. Immun. 59:4310-4317.
7. Felsenfeld, O. 1974. The survival of cholera vibrios, p. 359-366. In D. Barua and W. Burrows (ed.), Cholera. The W.B. Saunders Co., Philadelphia, Pa.
8. Heidelberg, J. F., J. A. Eisen, W. C Nelson, R. A. Clayton, M. L. Gwinn, R. J. Dodson, D. H. Haft, E. K. Hickey, J. D. Peterson, L. Umayam, S. R. Gill, K. E. Nelson, T. D. Read, H. Tettelin, D. Richardson, M. D. Ermolaeva, J. Vamathevan, S. Bass, H. Qin, I. Dragoi, P. Sellers, L. McDonald, T. Utterback, R. D. Fleishmann, W. C. Nierman, and O. White. 2000. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406: 477-483.
9. Islam, M. S., B. S. Drasar, and R. B. Sack. 1994. The aquatic flora and fauna as reservoirs of Vibrio cholerae: a review. J. Diarrhoeal Dis. Res. 12:87-96.
10. Jobling, M. G., and R. K. Holmes. 1997. Characterization of hapR, a positive regulator of the Vibrio cholerae HA/protease gene hap, and its identification as a functional homologue of the Vibrio harveyi luxR gene. Mol. Microbiol. 26:1023-1034.
11. Kaper, J. B., J. G. Morris, Jr., and M. M. Levine. 1995. Cholera Clin. Microbiol. Rev. 8:48-86.
12. 54-dependent transcriptional activators in Vibrio cholerae. J. Bacteriol. 180: 5256-5259.
13. Kustu, S., A. K. North, and D. S. Weiss. 1991. Prokaryotic transcriptional enhancers and enhancer-binding proteins. Trends Biochem. Sci. 16:397-402.
14. Kuzuyama, T., S. Takahashi, M. Takagi, and H. Seto. 2000. Characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, an enzyme involved in isopentenyl diphosphate biosynthesis, and identification of its catalytic amino acid residues. J. Biol. Chem. 275:19928-19932.
15. Kwon, V. M., and S. C. Ricke. 2000. Efficient amplification of multiple transposon flanking sequences. J. Microbiol. Methods 41:195-199.
16. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
17. McCarter, L. L. 1998. OpaR, a homolog of Vibrio harveyi LuxR, controls opacity of Vibrio parahaemolyticus. J. Bacteriol. 180:3166-3173.
18. Menard, R., P. J. Sansonetti, and C. Parsot. 1993. Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J. Bacteriol. 175:5899-5906.
19. Mizunoe, Y., S. N. Wai, A. Takede, and S.-I. Yoshida. 1999. Isolation and characterization of rugose form of Vibrio cholerae O139 strain MO10. Infect. Immun. 67:958-963.
20. 54 bacterial enhancer-binding protein family: mechanism of action and phylogenetic relationship of their functional domains. J. Bacteriol. 175:6067-6074.
21. Ochman, H., A. S. Gerber, and D. L. Hartl. 1988. Genetic applications of an inverse polymerase chain reaction. Genetics 120:621-623.
22. Pal, S. C. 1992. Laboratory diagnosis, p. 229-251. In D. Barua and W. B. Greenough III (ed.), Cholera. Plenum Medical Book Co., New York, N. Y.
23. Parker, J. D., P. S. Rabinovitch, and G. C. Burmer. 1991. Targeted gene walking polymerase chain reaction. Nucleic Acids Res. 19:3055-3060.
24. Prashar, Y., and S. M. Weissman. 1996. Analysis of differential gene expression by display of 3′ end restriction fragments of cDNAs. Proc. Natl. Acad. Sci. USA 93:659-663.
25. Quail, M. A., D. J. Haydon, and J. R. Guest. 1994. The pdhR-aceEF-lpd operon of Escherichia coli expresses the pyruvate dehydrogenase complex. Mol. Microbiol. 12:95-104.
26. Rhee, K. Y., M. Opel, E. Ito, S.-P. Hung, S. M. Arfin, and G. W. Hatfield. 1999. Transcriptional coupling between the divergent promoters of a prototypic LysR-type regulatory system, the ilvYC operon of Escherichia coli. Proc. Natl. Acad. Sci. USA 96:14294-14299.
27. Rice, E. W., C. H. Johnson, R. M. Clark, K. R. Fox, D. J. Reasoner, M. E. Dunnigan, P. Panigrahi, J. A. Johnson, and J. G. Morris, Jr. 1993 Vibrio cholerae O1 can assume a "rugose" survival form that resists killing by chlorine, yet retains virulence. Int. J. Environ. Health Res. 3:89-98.
28. Roszak, D. B., and R. R. Colwell. 1987. Survival strategies of bacteria in the natural environment. Microbiol. Rev. 51:365-379.
29. Sandkvist, M., L. O. Michel, L. P. Hough, V. M. Morales, M. Bagdasarian, M. Koomey, V. J. DeRita, and M. Bagdasarian. 1997. General secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in V. cholerae. J. Bacteriol. 179:6994-7003.
30. Schell, M. A. 1993. Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol. 47:597-626.
31. Shyamala, V., and G. F. Ames. 1989. Genome walking by single-specific-primer polymerase chain reaction. Gene 84:1-8.
32. Stoker, K., W. N. Reijnders, L. F. Oltmann, and A. H. Stouthamer. 1989. Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12. J. Bacteriol. 171:4448-4456.
33. Taylor, R. K., C. Manoil, and J. J. Mekalanos. 1989. Broad-host-range vectors for delivery of TnphoA: use in genetic analysis of secreted virulence determinants of Vibrio cholerae. J. Bacteriol. 171:1870-1878.
34. Wai, S. N., Y. Mizunoe, A. Takade, S.-I. Kawabata, and S.-I. Yoshida. 1998. Vibrio cholerae O1 strain TSI-4 produces the exopolysaccharide materials that determine colony morphology, stress resistance, and biotilm formation. Appl. Environ. Microbiol. 64:3648-3655.
35. Wai, S. N., Y. Mizunoe, and S.-I. Yoshida. 1999. How Vibrio cholerae survive during starvation. FEMS Microbiol. Lett. 180:123-131.
36. Watnick, P. I., K. J. Fuller, and R. Kolter. 1999. A role for the mannose sensitive hemagglutinin in biofilm formation by Vibrio cholerae E1 Tor. J. Bacteriol. 181:3606-3609.
37. Watnick, P. I., and R. Kolter. 1999. Steps in the development of a Vibrio cholerae E1 Tor biofilm. Mol. Microbiol. 34:586-595.
38. Watnick, P. I., and R. Kolter. 2000. Biofilm, city of microbes. J. Bacteriol. 182:2675-2679.
39. White, P. B. 1938. The rugose variant of vibrios. J. Pathol. Bacteriol. 46:1-6.
40. White, P. B. 1940. The characteristic hapten and antigen of rugose races of cholera and E1 Tor vibrios. J. Pathol. Bacteriol. 50:160-164.
41. Xu, H.-S., N. Roberts, F. L. Singleton, R. W. Attwell, D. J. Grimes, and R. R. Colwell. 1982. Survival and viability of non-culturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb. Ecol. 8:313-323.
42. Yildiz, F. H., and G. K. Schoolnik. 1999. Vibrio cholerae O1 E1 Tor: identification of a gene cluster required for the rugose colony type, exopolysaccharide production, chlorine resistance, and biofilm formation. Proc. Natl. Acad. Sci. USA 96:4028-4033.