A high-throughput genome-walking method and its use for cloning unknown flanking sequences

Analytical Biochemistry

Volumn 381, Issue 2, 2008, Pages 248-253

  Reddy, Palakolanu Sudhakar ^a   Mahanty, Srikrishna ^a   Kaul, Tanushri ^a   Nair, Suresh ^a   Sopory, Sudhir K ^a   Reddy, Malireddy K ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

Multiple displacement amplification (MDA); PCR based genome walk; Phi29 DNA polymerase; Whole genome amplification (WGA)

Indexed keywords

BINDING SITES; CLONE CELLS; CLONING; DNA; GENES; POLYMERS;

DEGENERATE PRIMERS; FLANKING REGIONS; FLANKING SEQUENCE; MULTIPLE DISPLACEMENT AMPLIFICATIONS; PCR AMPLIFICATION; PCR-BASED GENOME WALK; PHI29 DNA POLYMERASE; WHOLE GENOME AMPLIFICATIONS;

POLYMERASE CHAIN REACTION;

DNA POLYMERASE; PRIMER DNA;

ARTICLE; DNA FLANKING REGION; DNA SYNTHESIS; GENE AMPLIFICATION; GENE LOCUS; GENE SEQUENCE; GENOME; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 50049089040     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.07.012     Document Type: Article

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