Rapid isolation and functional analysis of promoter sequences of the nitrate reductase gene from Chlorella ellipsoidea

Journal of Applied Phycology

Volumn 16, Issue 1, 2004, Pages 11-16

  Wang, Peng ^a   Sun, Yongru ^a   Li, Xia ^a   Zhang, Liming ^a   Li, Wenbin ^a   Wang, Yiqin ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

Author keywords

Chlorella ellipsoidea; GUS gene; Nitrate reductase gene; Promoter elements; Thermal asymmetric interlaced (TAIL) PCR; Transient expression

Indexed keywords

GENE EXPRESSION;

'CHLORELLA' ELLIPSOIDEA; ALGAE; EUKARYOTA;

EID: 1842478180     PISSN: 09218971     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:JAPH.0000019048.56489.3c     Document Type: Article

References (26)

1. Bradford MM (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal. Biochem. 72: 248-254.
2. Brown LM (1982) Production of axenic cultures of algae by an osmotic method. Phycologia 21: 408-410.
3. Campbell WH, Kinghorn JR (1990) Functional domains of assimilatory nitrate reductase and nitrite reductase. Trends Biochem. Sci. 15: 315-319.
4. Chen Y, Wang YQ, Zhang LM, Sun YR, Li WB (2001) Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells. Curr. Genet. 39: 365-370.
5. Dawson HN, Burlingame R, Cannons AC (1997) Stable transformation of Chlorella: rescue of nitrate reductase-deficient mutants with the nitrate reductase gene. Curr. Micro. 35: 356-362.
6. Dawson HN, Pendleton LC, Solomonson LP, Cannons AC (1996) Cloning and characterization of the nitrate reductase-encoding gene from Chlorella vulgaries: structure and identification of transcription start points and initiator sequences. Gene 171: 139-145.
7. 4 is required for their synergistic activation of gene expression in Neurospora crassa. Mol. Cell Biol. 18: 3983-3990.
8. 3, the nitrate reductase structural gene of Neurospora crassa. J. Bact. 170: 657-661.
9. Hahn SA, Schutte M, Hooue A, Moskaluk CA, Dacosta LT, Rozenblum E, Weinstein CL, Fischer A, Yeo CJ, Hruban RH, Kern SE (1996) A candidate tumor-suppressor gene at human-chromsome 18q21.1. Science 271: 350-353.
10. Jarvis EE, Brown LM (1991) Transient expression of firefly luciferase in protoplast of the green alga Chlorella ellipsoidea. Curr. Genet. 19: 317-321.
11. Jefferson RA (1987) Assaying chimeric genes in plant: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387-405.
12. Liu YG, Huang N (1998) Efficient amplification of insert end sequences from bacterial artificial chromosome clones by thermal asymmetric interlaced PCR. Plant Mol. Biol. Rep. 16: 175-181.
13. Liu YG, Mitsukawa N, Oosumi T, Whittier RF (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J. 8: 457-463.
14. Liu YG, Whittier RF (1995) Thermal asymmetric interlaced PCR automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25: 674-681.
15. Loppes R, Ohresser M, Radoux M, Matagne RF (1999) Transcriptional regulation of Nit1 gene encoding nitrate reductase in Chlamydomonas reinhardtii: effect of various environmental factors on the expression of a reporter gene under the control of the Nit1 promoter. Plant Mol. Biol. 41: 701-711.
16. Maruyama M, Horakova I, Honda H, Xing XH, Shiragimi N, Unno H (1994) Introduction of foreign DNA into Chlorella sacchamphila by electroporation. Biotech. Tech. 8: 821-826.
17. Marzluf GA (1997) Genetic regulation of nitrogen metabolism in the fungi. Microbiol. Mol. Biol. Rev. 61: 17-32.
18. Murray MG, Thompson WF ( 1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325.
19. Popova AF, Kordium EL (1997) The characteristics of the structural organization of Chlorella cells cultured over 1 year under space flight conditions. Tsitol. Genet. 31: 3-9.
20. Richard L, Hawkins, Nakamura M (1999) Expression of growth hormone by the eukaryotic alga, Chlorella. Curr. Microb. 38: 335-341.
21. Schreiner M, Geisert M, Oed M, Arendes J, Gungerich V, Breter HJ, Stuber K, Weinblum D (1995) Nucleotide phylogenetic relation-ship of the green alga Nanochlorum eukaryotum deduced from its chloroplast rRNA sequences. J. mol. Evol. 40: 428-442.
22. Smith D, Yanai Y, Liu YG, Ishiguro S, Okada K, Shibata D, Whittier RF, Fedoroff NV (1996) Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis. Plant J. 10: 721-732.
23. Solomonson LP, Barber MJ (1990) Assimilatory nitrate reductase: functional properties and regulation. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41: 240-253.
24. Tomsett AB, Dunn-Coleman NS, Garrett RH (1981) The regulation of nitrate assimilation in Neurospora crassa, the isolation and genetic analysis of Nmr-1 mutants. Mol. Gen. Genet. 182: 229-233.
25. Tsugeki R, Kochieva EZ, Fedoroff NV (1996) A transposon insertion in the Arabidopsis SSR16 gene causes an embryo-defective lethal mutation. Plant J. 10: 479-489.
26. Xiao X, Fu Y Marzluf GA (1995) The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits the DNA-binding activity of the positive-acting nitrogen regulatory protein Nit2. Biochem. 34: 8861-8868.