Rapid amplification and cloning of Tn5 flanking fragments by inverse PCR

Letters in Applied Microbiology

Volumn 31, Issue 2, 2000, Pages 149-153

  Huang, G ^a   Zhang, L ^b   Birch, R G ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CLONING; DNA; INVERSE PROBLEMS; POLYMERASE CHAIN REACTION;

AMPLICONS; CONDITION; FLANKING SEQUENCE; GENOMICS; INVERSE PCR; MONOMERICS; PCR REACTIONS; RESTRICTION ENZYMES; SIMPLE APPROACH; SOUTHERN BLOT ANALYSIS;

DNA SEQUENCES;

DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; CLONING; CONTROLLED STUDY; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; TRANSPOSON; XANTHOMONAS;

XANTHOMONAS ALBILINEANS;

EID: 0033837287     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2000.00781.x     Document Type: Article

References (13)

1. Preliminary characterization of an antibiotic produced by Xanthomonas albilineans which inhibits DNA synthesis in Escherichia coli
2. Directional cloning of DNA fragments at a large distance from an initial probe: A circularization method
3. The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids - A review
4. An alternative inverse PCR (IPCR) method to amplify DNA sequences flanking Tn5 transposon insertions
5. Genetic applications of an inverse polymerase chain reaction
6. A single oligonucleotide can be used to rapidly isolate DNA sequences flanking a transposon Tn5 insertion by the polymerase chain reaction
7. DNA sequencing with chain-terminating inhibitors
8. Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus
9. High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon
10. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteria
11. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences
12. Genes for albicidin biosynthesis and resistance span at least 69 kb in the genome of Xanthomonas albilineans