Antigen retrieval causes protein unfolding: Evidence for a linear epitope model of recovered immunoreactivity

Journal of Histochemistry and Cytochemistry

Volumn 59, Issue 4, 2011, Pages 366-381

  Fowler, Carol B ^a,b   Evers, David L ^a,b   O'Leary, Timothy J ^a,b   Mason, Jeffrey T ^a  

^a ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Antigen retrieval; Circular dichroism; Formaldehyde; Formalin fixed paraffin embedded tissue; Myoglobin; Protein unfolding; Ribonuclease a

Indexed keywords

ANTIGEN; DODECYL SULFATE SODIUM; EPITOPE; FORMALDEHYDE; PROTEIN; CROSS LINKING REAGENT; FIXATIVE; PARAFFIN;

ANTIGEN RETRIEVAL; ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; HEATING; HIGH TEMPERATURE PROCEDURES; IMMUNOREACTIVITY; INCUBATION TEMPERATURE; MOLECULAR MODEL; NONHUMAN; POLARIMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN CROSS LINKING; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; PROTEIN UNFOLDING; THERMOSTABILITY; CHEMISTRY; HEAT;

ANTIGENS; CIRCULAR DICHROISM; CROSS-LINKING REAGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPES; FIXATIVES; FORMALDEHYDE; HOT TEMPERATURE; PARAFFIN EMBEDDING; PROTEIN DENATURATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEIN UNFOLDING; PROTEINS;

EID: 79955703529     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/0022155411400866     Document Type: Article

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