2D difference gel electrophoresis analysis of different time points during the course of neoplastic transformation of human mammary epithelial cells

Journal of Proteome Research

Volumn 10, Issue 2, 2011, Pages 447-458

  DeAngelis, J Tyson ^a   Li, Yuanyuan ^a   Mitchell, Natalie ^a   Wilson, Landon ^b   Kim, Helen ^a,b,c   Tollefsbol, Trygve O ^a,b  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c University of Alabama at Birmingham   (United States)

Author keywords

2D DIGE; Breast cancer; Human mammary epithelial cells; Ingenuity pathway analysis; Maspin; Metastasis; Neoplastic transformation

Indexed keywords

CHAPERONIN CONTAINING TCP1; CYTOKERATIN 1; CYTOKERATIN 7; CYTOSKELETON PROTEIN; FK 506 BINDING PROTEIN; GELSOLIN; HEAT SHOCK PROTEIN 4A; MASPIN; RIBOSOME PROTEIN; RIBOSOME PROTEIN P0; STATHMIN; TUMOR MARKER; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BREAST CANCER; BREAST CARCINOGENESIS; BREAST EPITHELIUM; CANCER CELL CULTURE; CELL MOTILITY; CELL TRANSFORMATION; CONTROLLED STUDY; DIFFERENCE GEL ELECTROPHORESIS; DOWN REGULATION; FEMALE; GEL ELECTROPHORESIS; HUMAN; HUMAN CELL; MALIGNANT TRANSFORMATION; METASTASIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN MODIFICATION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; BREAST NEOPLASMS; CELL LINE, TRANSFORMED; CELL TRANSFORMATION, NEOPLASTIC; CELLS, CULTURED; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FEMALE; GENE TRANSFER TECHNIQUES; HUMANS; MAMMARY GLANDS, HUMAN; MICE; MICE, INBRED BALB C; MICE, NUDE; MODELS, BIOLOGICAL; NEOPLASM METASTASIS; PROTEOME; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SERPINS; SIGNAL TRANSDUCTION; TRANSPLANTATION, HETEROLOGOUS;

EID: 79955456953     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr100533k     Document Type: Article

References (48)

1. Hahn, W.; Counter, C; Lundberg, A.; Beijersbergen, R.; Brooks, M.; Weinberg, R. Creation of human tumour cells with defined genetic elements. Nature 1999, 400 (6743), 464-8. (Pubitemid 29361801)
2. Elenbaas, B.; Spirio, L.; Koerner, F.; Fleming, M. D.; Zimonjic, D. B.; Donaher, J. L.; Popescu, N. C.; Hahn, W. C.; Weinberg, R. A. Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells. Genes Dev. 2001, 15 (1), 50-65. (Pubitemid 32060682)
3. Hochedlinger, K.; Plath, K. Epigenetic reprogramming and induced pluripotency. Development 2009, 136 (4), 509-23.
4. Hahn, W.; Dessain, S.; Brooks, M.; King, J.; Elenbaas, B.; Sabatini, D.; DeCaprio, J.; Weinberg, R. Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Mol. Cell. Biol. 2002, 22 (7), 2111-23. (Pubitemid 34224619)
5. Counter, C.; Hahn, W.; Wei, W.; Caddle, S.; Beijersbergen, R.; Lansdorp, P.; Sedivy, J.; Weinberg, R. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc. Natl. Acad. Sci. U.S.A. 1998, 95 (25), 14723-8. (Pubitemid 29003697)
6. Casillas, M. A., Jr.; Lopatina, N.; Andrews, L. G.; Tollefsbol, T. O. Transcriptional control of the DNA methyltransferases is altered in aging and neoplastically-transformed human fibroblasts. Mol. Cell. Biochem. 2003, 252, 33.
7. Jones, P.; Baylin, S. The fundamental role of epigenetic events in cancer. Nat. Rev. Genet. 2002, 3 (6), 415-28. (Pubitemid 34587077)
8. Hammond, S.; Ham, R.; Stampfer, M. Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract. Proc. Natl. Acad. Sci. U.S.A. 1984, 81 (17), 5435-9. (Pubitemid 14048762)
9. Stampfer, M.; Bartley, J. Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo[a]pyrene. Proc. Natl. Acad. Sci. U.S.A. 1985, 82 (8), 2394-8. (Pubitemid 15064494)
10. Pear, W. S.; Nolan, G. P.; Scott, M. L.; Baltimore, D. Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. U.S.A. 1993, 90 (18), 8392-6. (Pubitemid 23277674)
11. Hurst, D.; Xie, Y.; Edmonds, M.; Welch, D. Multiple forms of BRMS1 are differentially expressed in the MCF10 isogenic breast cancer progression model. Clin. Exp. Metastasis 2009, 26 (2), 89-96.
12. Santner, S.; Dawson, P.; Tait, L.; Soule, H.; Eliason, J.; Mohamed, A.; Wolman, S.; Heppner, G.; Miller, F. Malignant MCF10CA1 cell lines derived from premalignant human breast epithelial MCF10AT cells. Breast Cancer Res. Treat. 2001, 65 (2), 101-10. (Pubitemid 32193613)
13. Saldanha, S.; Andrews, L.; Tollefsbol, T. Analysis of telomerase activity and detection of its catalytic subunit, hTERT. Anal. Biochem. 2003, 315 (1), 1-21. (Pubitemid 36379101)
14. Chomczynski, P. Solubilization in formamide protects RNA from degradation. Nucleic Acids Res. 1992, 20 (14), 3791-2.
15. Kim, H.; Cope, M. B.; Herring, R.; Robinson, G.; Wilson, L.; Page, G. P.; Barnes, S. 2D difference gel electrophoresis of prepubertal and pubertal rat mammary gland proteomes. J. Proteome Res. 2008, 7 (11), 4638-50.
16. Livak, K.; Schmittgen, T. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25 (4), 402-8. (Pubitemid 34164012)
17. Ramachandran, C.; Fonseca, H.; Jhabvala, P.; Escalon, E.; Melnick, S. Curcumin inhibits telomerase activity through human telom-erase reverse transcritpase in MCF-7 breast cancer cell line. Cancer Lett. 2002, 184 (1), 1-6. (Pubitemid 34718100)
18. Shi, H. Y.; Zhang, W.; Liang, R.; Kittrell, F.; Templeton, N. S.; Medina, D.; Zhang, M. Modeling human breast cancer metastasis in mice: maspin as a paradigm. Histol. Histopathol. 2003, 18 (1), 201-6. (Pubitemid 36040461)
19. Wang, D.; Park, J.; Chu, J.; Krakowski, A.; Luo, K.; Chen, D.; Li, S. Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor beta1 stimulation. J. Biol. Chem. 2004, 279 (42), 43725-34. (Pubitemid 39390677)
20. Wang, S. E.; Narasanna, A.; Whitell, C. W.; Wu, F. Y.; Friedman, D. B.; Arteaga, C. L. Convergence of p53 and Transforming Growth Factor beta (TGFbeta) Signaling on Activating Expression of the Tumor Suppressor Gene maspin in Mammary Epithelial Cells. J. Biol. Chem. 2007, 282 (8), 5661-9. (Pubitemid 47093781)
21. Koch, H.; Zhang, R.; Verdoodt, B.; Bailey, A.; Zhang, C.; Yates, J. r.; Menssen, A.; Hermeking, H. Large-scale identification of c-MYC- associated proteins using a combined TAP/MudPIT approach. Cell Cycle 2007, 6 (2), 205-17. (Pubitemid 46232502)
22. Wilson, C. A.; Cajulis, E. E.; Green, J. L.; Olsen, T. M.; Chung, Y. A.; Damore, M. A.; Dering, J.; Calzone, F. J.; Slamon, D. J. HER-2 overexpression differentially alters transforming growth factor-beta responses in luminal versus mesenchymal human breast cancer cells. Breast Cancer Res. 2005, 7 (6), R1058-79.
23. Stasyk, T.; Dubrovska, A.; Lomnytska, M.; Yakymovych, I.; Wernstedt, C.; Heldin, C. H.; Hellman, U.; Souchelnytskyi, S. Phosphoproteome Profiling of Transforming Growth Factor (TGF)-{beta} Signaling: Abrogation of TGF{beta}1-dependent Phosphorylation of Transcription Factor-II-I (TFII-I) Enhances Cooperation of TFII-I and Smad3 in Transcription. Mol. Biol. Cell 2005, 16 (10), 4765-80. (Pubitemid 41416459)
24. Ewing, R. M.; Chu, P.; Elisma, F.; Li, H.; Taylor, P.; Climie, S.; McBroom-Cerajewski, L.; Robinson, M. D.; O'Connor, L.; Li, M.; Taylor, R.; Dharsee, M.; Ho, Y.; Heilbut, A.; Moore, L.; Zhang, S.; Ornatsky, O.; Bukhman, Y. V.; Ethier, M.; Sheng, Y.; Vasilescu, J.; Abu-Farha, M.; Lambert, J. P.; Duewel, H. S.; Stewart, I. I.; Kuehl, B.; Hogue, K.; Colwill, K.; Gladwish, K.; Muskat, B.; Kinach, R.; Adams, S. L.; Moran, M. F.; Morin, G. B.; Topaloglou, T.; Figeys, D. Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol. Syst. Biol. 2007, 3, 89.
25. Lohr, K.; Moritz, C.; Contente, A.; Dobbelstein, M. p21/CDKN1A mediates negative regulation of transcription by p53. J. Biol. Chem. 2003, 278, 32507-16. (Pubitemid 37055688)
26. Abe, Y.; Oda-Sato, E.; Tobiume, K.; Kawauchi, K.; Taya, Y.; Okamoto, K.; Oren, M.; Tanaka, N. Hedgehog signaling overrides p53-mediated tumor suppression by activating Mdm2. Proc. Natl. Acad. Sci. U.S.A. 2008, 105 (12), 4838-43. (Pubitemid 351753833)
27. Dennler, S.; Andre, J.; Verrecchia, F.; Mauviel, A. Cloning of the human GLI2 Promoter: transcriptional activation bytransforming growth factor-beta via SMAD3/beta-catenin cooperation. J. Biol. Chem. 2009, 284 (46), 31523-31.
28. Araki, S.; Eitel, J. A.; Batuello, C. N.; Bijangi-Vishehsaraei, K.; Xie, X. J.; Danielpour, D.; Pollok, K. E.; Boothman, D. A.; Mayo, L. D. TGF-beta1-induced expression of human Mdm2 correlates with late-stage metastatic breast cancer. J. Clin. Invest. 2010, 120 (1), 290-302.
29. Sheng, S.; Carey, J.; Seftor, E. A.; Dias, L.; Hendrix, M. J.; Sager, R. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. U.S.A. 1996, 93 (21), 11669-74. (Pubitemid 26347180)
30. Cunningham, C. C; Vegners, R.; Bucki, R.; Funaki, M.; Korde, N.; Hartwig, J. H.; Stossel, T. P.; Janmey, P. A. Cell permeant poly-phosphoinositide- binding peptides that block cell motility and actin assembly. J. Biol. Chem. 2001, 276 (46), 43390-9.
31. Shehata, M.; Schwarzmeier, J. D.; Hilgarth, M.; Hubmann, R.; Duechler, M.; Gisslinger, H. TGF-betal induces bone marrow reticulin fibrosis in hairy cell leukemia. J. Clin. Invest. 2004, 113 (5), 676-85. (Pubitemid 38544160)
32. Kwiatkowski, D. J.; Stossel, T. P.; Orkin, S. H.; Mole, J. E.; Colten, H. R.; Yin, H. L. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature 1986, 323 (6087), 455-8. (Pubitemid 16026275)
33. Alli, E.; Bash-Babula, J.; Yang, J. M.; Hait, W. N. Effect of stathmin on the sensitivity to antimicrotubule drugs in human breast cancer. Cancer Res. 2002, 62 (23), 6864-9. (Pubitemid 35424074)
34. Baldassarre, G.; Belletti, B.; Nicoloso, M. S.; Schiappacassi, M.; Vecchione, A.; Spessotto, P.; Morrione, A.; Canzonieri, V.; Colombatti, A. p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion. Cancer Cell 2005, 7 (1), 51-63. (Pubitemid 40126382)
35. Vardouli, L.; Moustakas, A.; Stournaras, C. LIM-kinase 2 and Cofilin Phosphorylation Mediate Actin Cytoskeleton Reorganization Induced by Transforming Growth Factor-{beta}. J. Biol. Chem. 2005, 280 (12), 11448-57. (Pubitemid 40418454)
36. Steinmetz, M. O.; Jahnke, W.; Towbin, H.; García- Echeverría, C.; Voshol, H.; Müller, D.; van Oostrum, J. Phosphorylation disrupts the central helix in Op18/stathmin and suppresses binding to tubulin. EMBO Rep. 2001, 2 (6), 505-10. (Pubitemid 32611347)
37. Holmfeldt, P.; Stenmark, S.; Gullberg, M. Interphase-specific phosphorylation-mediated regulation of tubulin dimer partitioning in human cells. Mol. Biol. Cell 2007, 18 (5), 1909-17. (Pubitemid 46717570)
38. Gavert, N.; Sheffer, M.; Raveh, S.; Spaderna, S.; Shtutman, M.; Brabletz, T.; Barany, F.; Paty, P.; Notterman, D.; Domany, E.; Ben-Ze'ev, A. Expression of L1-CAM and ADAM10 in human colon cancer cells induces metastasis. Cancer Res. 2007, 67 (16), 7703-12. (Pubitemid 47281363)
39. Thor, A. D.; Edgerton, S. M.; Liu, S.; Moore, D. H.; Kwiatkowski, D. J. Gelsolin as a negative prognostic factor and effector of motility in erbB-2-positive epidermal growth factor receptor-positive breast cancers. Clin. Cancer Res. 2001, 7 (8), 2415-24. (Pubitemid 32751644)
40. Ashida, S.; Nakagawa, H.; Katagiri, T.; Furihata, M.; Iiizumi, M.; Anazawa, Y.; Tsunoda, T.; Takata, R.; Kasahara, K.; Miki, T.; Fujioka, T.; Shuin, T.; Nakamura, Y. Molecular features of the transition from prostatic intraepithelial neoplasia (PIN) to prostate cancer: genome-wide gene-expression profiles of prostate cancers and PINs. Cancer Res. 2004, 64 (17), 5963-72. (Pubitemid 39129392)
41. Mani, S.; Guo, W.; Liao, M.; Eaton, E.; Ayyanan, A.; Zhou, A.; Brooks, M.; Reinhard, F.; Zhang, C.; Shipitsin, M.; Campbell, L.; Polyak, K.; Brisken, C.; Yang, J.; Weinberg, R. The epithelial- mesenchymal transition generates cells with properties of stem cells. Cell 2008, 133 (4), 704-15. (Pubitemid 351636299)
42. Katoh, Y.; Katoh, M. Hedgehog target genes: mechanisms of carcinogenesis induced by aberrant hedgehog signaling activation. Curr. Mol. Med. 2009, 9 (7), 873-86.
43. Rana, S.; Maples, P.; Senzer, N.; Nemunaitis, J. Stathmin 1: a novel therapeutic target for anticancer activity. Expert Rev. Anticancer Ther. 2008, 8 (9), 1461-70.
44. Renz, M.; Langowski, J. Dynamics of the CapG actin-binding protein in the cell nucleus studied by FRAP and FCS. Chromosome Res. 2008, 16 (3), 427-37. (Pubitemid 351644865)
45. Teoh, S. S.; Whisstock, J. C.; Bird, P. I. Maspin (serpinb5) is an obligate intracellular serpin. J. Biol. Chem. 2010, 285, 10862-9.
46. Chan, H.; Gharbi, S.; Gaffney, P.; Cramer, R.; Waterfield, M.; Timms, J. Proteomic analysis of redox- and ErbB2-dependent changes in mammary luminal epithelial cells using cysteine- and lysine- labelling two-dimensional difference gel electrophoresis. Proteomics 2005, 5 (11), 2908-26. (Pubitemid 41059768)
47. Selicharová, I.; Smutná, K.; Sanda, M.; Ubik, K.; Matousková, E.; Bursíková, E.; Brozová, M.; Vydra, J.; Jirácek, J. 2-DE analysis of a new human cell line EM-G3 derived from breast cancer progenitor cells and comparison with normal mammary epithelial cells. Proteomics 2007, 7 (9), 1549-59. (Pubitemid 46775885)
48. Slamon, D. J.; Clark, G. M.; Wong, S. G.; Levin, W. J.; Ullrich, A.; McGuire, W. L. Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 1987, 235 (4785), 177-82. (Pubitemid 17231334)