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50) was determined from the dose-response curves according to the inhibition ratio for each concentration. In vitro antibacterial activity was studied with broth microdilution method. Staphylococcus aureus, Escherichia coli, Monilia albican and Aspergillus niger were chose for this study. The minimum inhibitory concentration (MIC) was measured. All the experiment was carried out in triplicate.
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