Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate

PLoS ONE

Volumn 6, Issue 4, 2011, Pages

  Coltri, Patricia ^a,b   Effenberger, Kerstin ^a   Chalkley, Robert J ^c   Burlingame, A L ^c   Jurica, Melissa S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

C COMPLEX PROTEIN; NTC PROTEIN; PROTEIN; PROTEIN PRP19; SMALL NUCLEAR RNA; SMALL NUCLEAR RNA U2; SMALL NUCLEAR RNA U5; SMALL NUCLEAR RNA U6; UNCLASSIFIED DRUG; UREA; GLYCEROL; MULTIPROTEIN COMPLEX;

ARTICLE; COMPLEX FORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; EXON; GENETIC ASSOCIATION; HUMAN; HUMAN CELL; INTRON; PROTEIN RNA BINDING; PROTEIN STABILITY; SPLICEOSOME; CHEMISTRY; CONFORMATION; DRUG EFFECT; GENETICS; HELA CELL; MASS SPECTROMETRY; METABOLISM; PROTEIN BINDING; RNA SPLICING;

MAMMALIA;

EXONS; GLYCEROL; HELA CELLS; HUMANS; INTRONS; MASS SPECTROMETRY; MULTIPROTEIN COMPLEXES; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; RNA SPLICING; RNA, SMALL NUCLEAR; SPLICEOSOMES; UREA;

EID: 79955402909     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0019061     Document Type: Article

References (28)

1. Wahl MC, Will CL, Luhrmann R, (2009) The spliceosome: design principles of a dynamic RNP machine. Cell 136: 701-718.
2. Brow DA, (2002) Allosteric cascade of spliceosome activation. Annu Rev Genet 36: 333-360.
3. Smith DJ, Konarska MM, Query CC, (2009) Insights into branch nucleophile positioning and activation from an orthogonal pre-mRNA splicing system in yeast. Mol Cell 34: 333-343.
4. Staley JP, Guthrie C, (1998) Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell 92: 315-326.
5. Perriman RJ, Ares M Jr, (2007) Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing. Genes Dev 21: 811-820.
6. Wu J, Manley JL, (1989) Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing. Genes Dev 3: 1553-1561.
7. Wu JA, Manley JL, (1991) Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA. Nature 352: 818-821.
8. Dybkov O, Will CL, Deckert J, Behzadnia N, Hartmuth K, et al. (2006) U2 snRNA-protein contacts in purified human 17S U2 snRNPs and in spliceosomal A and B complexes. Mol Cell Biol 26: 2803-2816.
9. Hogg R, McGrail JC, O'Keefe RT, (2010) The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing. Biochem Soc Trans 38: 1110-1115.
10. Chan SP, Cheng SC, (2005) The Prp19-associated complex is required for specifying interactions of U5 and U6 with pre-mRNA during spliceosome activation. J Biol Chem 280: 31190-31199.
11. Chan SP, Kao DI, Tsai WY, Cheng SC, (2003) The Prp19p-associated complex in spliceosome activation. Science 302: 279-282.
12. Villa T, Guthrie C, (2005) The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing. Genes Dev 19: 1894-1904.
13. Grainger RJ, Beggs JD, (2005) Prp8 protein: at the heart of the spliceosome. Rna 11: 533-557.
14. Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ, (2002) Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. Rna 8: 426-439.
15. Bantscheff M, Schirle M, Sweetman G, Rick J, Kuster B, (2007) Quantitative mass spectrometry in proteomics: a critical review. Anal Bioanal Chem 389: 1017-1031.
16. Gozani O, Feld R, Reed R, (1996) Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A. Genes Dev 10: 233-243.
17. Yan D, Ares M Jr, (1996) Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol Cell Biol 16: 818-828.
18. Kramer A, Gruter P, Groning K, Kastner B, (1999) Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP. J Cell Biol 145: 1355-1368.
19. Bessonov S, Anokhina M, Will CL, Urlaub H, Luhrmann R, (2008) Isolation of an active step I spliceosome and composition of its RNP core. Nature 452: 846-850.
20. Lardelli RM, Thompson JX, Yates JR 3rd, Stevens SW, (2010) Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing. Rna 16: 516-528.
21. Mayas RM, Maita H, Staley JP, (2006) Exon ligation is proofread by the DExD/H-box ATPase Prp22p. Nat Struct Mol Biol 13: 482-490.
22. Schwer B, (2008) A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release. Mol Cell 30: 743-754.
23. Tseng CK, Cheng SC, (2008) Both catalytic steps of nuclear pre-mRNA splicing are reversible. Science 320: 1782-1784.
24. Hirose T, Ideue T, Nagai M, Hagiwara M, Shu MD, et al. (2006) A spliceosomal intron binding protein, IBP160, links position-dependent assembly of intron-encoded box C/D snoRNP to pre-mRNA splicing. Mol Cell 23: 673-684.
25. Das R, Zhou Z, Reed R, (2000) Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell 5: 779-787.
26. Jurica MS, Sousa D, Moore MJ, Grigorieff N, (2004) Three-dimensional structure of C complex spliceosomes by electron microscopy. Nat Struct Mol Biol 11: 265-269.
27. Konarska MM, Sharp PA, (1987) Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell 49: 763-774.
28. Chalkley RJ, Baker PR, Medzihradszky KF, Lynn AJ, Burlingame AL, (2008) In-depth analysis of tandem mass spectrometry data from disparate instrument types. Mol Cell Proteomics 7: 2386-95.