메뉴 건너뛰기




Volumn 11, Issue , 2011, Pages

1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

Author keywords

[No Author keywords available]

Indexed keywords

1 ,2-PROPANEDIOL; AMPLICONS; BLOOD SAMPLES; CONTINUOUS MONITORING; DNA AMPLIFICATION; DNA FRAGMENT; DNA MELTING; DNA POLYMERASE; DNA-TEMPLATE; EXCELLENT PERFORMANCE; GENE EXPRESSION ANALYSIS; GENOMIC DNA; GENOTYPING; MEDIUM SIZE; MUTATION FREQUENCY; QUANTITATIVE REAL TIME PCR; SALT COMPOSITION; SYBR GREEN I; WHOLE BLOOD;

EID: 79955113113     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-41     Document Type: Article
Times cited : (36)

References (42)
  • 1
    • 13444288289 scopus 로고    scopus 로고
    • International University Line. La Jolla, California
    • Bustin SA. A-Z of Quantitative PCR 2004, International University Line. La Jolla, California.
    • (2004) A-Z of Quantitative PCR
    • Bustin, S.A.1
  • 2
    • 3142708522 scopus 로고    scopus 로고
    • Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
    • 10.1093/nar/gnh101, 484200, 15249599
    • Zipper H, Brunner H, Bernhagen J, Vitzthum F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res 2004, 32(12):e103. 10.1093/nar/gnh101, 484200, 15249599.
    • (2004) Nucleic Acids Res , vol.32 , Issue.12
    • Zipper, H.1    Brunner, H.2    Bernhagen, J.3    Vitzthum, F.4
  • 3
    • 38649136194 scopus 로고    scopus 로고
    • Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
    • 10.1186/1472-6750-7-76, 2213645, 17996102
    • Mao F, Leung WY, Xin X. Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications. BMC Biotechnol 2007, 7:76. 10.1186/1472-6750-7-76, 2213645, 17996102.
    • (2007) BMC Biotechnol , vol.7 , pp. 76
    • Mao, F.1    Leung, W.Y.2    Xin, X.3
  • 4
    • 15544371286 scopus 로고    scopus 로고
    • Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis
    • 10.1016/j.ab.2005.01.046, 15802126
    • Monis PT, Giglio S, Saint CP. Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Anal Biochem 2005, 340(1):24-34. 10.1016/j.ab.2005.01.046, 15802126.
    • (2005) Anal Biochem , vol.340 , Issue.1 , pp. 24-34
    • Monis, P.T.1    Giglio, S.2    Saint, C.P.3
  • 5
    • 50849135851 scopus 로고    scopus 로고
    • Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
    • 10.1093/nar/gkn421, 2528177, 18606615
    • Shaik GM, Draberova L, Draber P, Boubelik M, Draber P. Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I. Nucleic Acids Res 2008, 36(15):e93. 10.1093/nar/gkn421, 2528177, 18606615.
    • (2008) Nucleic Acids Res , vol.36 , Issue.15
    • Shaik, G.M.1    Draberova, L.2    Draber, P.3    Boubelik, M.4    Draber, P.5
  • 6
    • 14744306436 scopus 로고
    • Simultaneous amplification and detection of specific DNA sequences
    • 10.1038/nbt0492-413, 1368485
    • Higuchi R, Dollinger G, Walsh PS, Griffith R. Simultaneous amplification and detection of specific DNA sequences. Biotechnology (N Y ) 1992, 10(4):413-7. 10.1038/nbt0492-413, 1368485.
    • (1992) Biotechnology (N Y ) , vol.10 , Issue.4 , pp. 413-417
    • Higuchi, R.1    Dollinger, G.2    Walsh, P.S.3    Griffith, R.4
  • 7
    • 0031022694 scopus 로고    scopus 로고
    • Continuous fluorescence monitoring of rapid cycle DNA amplification
    • Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques 1997, 22(1):130-8.
    • (1997) BioTechniques , vol.22 , Issue.1 , pp. 130-138
    • Wittwer, C.T.1    Herrmann, M.G.2    Moss, A.A.3    Rasmussen, R.P.4
  • 8
    • 0029076080 scopus 로고
    • Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater
    • 10.1006/abio.1995.1404, 7485974
    • Ishiguro T, Saitoh J, Yawata H, Yamagishi H, Iwasaki S, Mitoma Y. Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater. Anal Biochem 1995, 229(2):207-13. 10.1006/abio.1995.1404, 7485974.
    • (1995) Anal Biochem , vol.229 , Issue.2 , pp. 207-213
    • Ishiguro, T.1    Saitoh, J.2    Yawata, H.3    Yamagishi, H.4    Iwasaki, S.5    Mitoma, Y.6
  • 9
    • 0038476170 scopus 로고    scopus 로고
    • A new minor groove binding asymmetric cyanine reporter dye for real-time PCR
    • 10.1093/nar/gng045, 153757, 12682380
    • Bengtsson M, Karlsson HJ, Westman G, Kubista M. A new minor groove binding asymmetric cyanine reporter dye for real-time PCR. Nucleic Acids Res 2003, 31(8):e45. 10.1093/nar/gng045, 153757, 12682380.
    • (2003) Nucleic Acids Res , vol.31 , Issue.8
    • Bengtsson, M.1    Karlsson, H.J.2    Westman, G.3    Kubista, M.4
  • 10
    • 0038359432 scopus 로고    scopus 로고
    • High-resolution genotyping by amplicon melting analysis using LCGreen
    • 10.1373/49.6.853, 12765979
    • Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003, 49(6):853-60. 10.1373/49.6.853, 12765979.
    • (2003) Clin Chem , vol.49 , Issue.6 , pp. 853-860
    • Wittwer, C.T.1    Reed, G.H.2    Gundry, C.N.3    Vandersteen, J.G.4    Pryor, R.J.5
  • 11
    • 36148978172 scopus 로고    scopus 로고
    • Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature
    • 10.1093/nar/gkm671, 2095797, 17897966
    • Gudnason H, Dufva M, Bang DD, Wolff A. Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature. Nucleic Acids Res 2007, 35(19):e127. 10.1093/nar/gkm671, 2095797, 17897966.
    • (2007) Nucleic Acids Res , vol.35 , Issue.19
    • Gudnason, H.1    Dufva, M.2    Bang, D.D.3    Wolff, A.4
  • 12
    • 40749117206 scopus 로고    scopus 로고
    • Rapid detection of carriers with BRCA1 and BRCA2 mutations using high resolution melting analysis
    • 10.1186/1471-2407-8-59, 2266761, 18298804
    • Takano EA, Mitchell G, Fox SB, Dobrovic A. Rapid detection of carriers with BRCA1 and BRCA2 mutations using high resolution melting analysis. BMC Cancer 2008, 8:59. 10.1186/1471-2407-8-59, 2266761, 18298804.
    • (2008) BMC Cancer , vol.8 , pp. 59
    • Takano, E.A.1    Mitchell, G.2    Fox, S.B.3    Dobrovic, A.4
  • 13
    • 44449150364 scopus 로고    scopus 로고
    • A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars
    • 10.1186/1746-4811-4-8, 2396621, 18489740
    • Mackay JF, Wright CD, Bonfiglioli RG. A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars. Plant Methods 2008, 4:8. 10.1186/1746-4811-4-8, 2396621, 18489740.
    • (2008) Plant Methods , vol.4 , pp. 8
    • Mackay, J.F.1    Wright, C.D.2    Bonfiglioli, R.G.3
  • 14
    • 0028211009 scopus 로고
    • PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates
    • 10.1073/pnas.91.6.2216, 43341, 8134376
    • Barnes WM. PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates. Proc Natl Acad Sci USA 1994, 91(6):2216-20. 10.1073/pnas.91.6.2216, 43341, 8134376.
    • (1994) Proc Natl Acad Sci USA , vol.91 , Issue.6 , pp. 2216-2220
    • Barnes, W.M.1
  • 16
    • 0343938892 scopus 로고    scopus 로고
    • Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR
    • 88721, 10618113
    • Al-Soud WA, Jönsson LJ, Rådström P. Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR. J Clin Microbiol 2000, 38(1):345-50. 88721, 10618113.
    • (2000) J Clin Microbiol , vol.38 , Issue.1 , pp. 345-350
    • Al-Soud, W.A.1    Jönsson, L.J.2    Rådström, P.3
  • 17
    • 0035126283 scopus 로고    scopus 로고
    • Purification and characterization of PCR-inhibitory components in blood cells
    • 10.1128/JCM.39.2.485-493.2001, 87763, 11158094
    • Al-Soud WA, Rådström P. Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 2001, 39(2):485-93. 10.1128/JCM.39.2.485-493.2001, 87763, 11158094.
    • (2001) J Clin Microbiol , vol.39 , Issue.2 , pp. 485-493
    • Al-Soud, W.A.1    Rådström, P.2
  • 18
    • 3042614052 scopus 로고    scopus 로고
    • Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of Taq polymerase by the disaccharide trehalose
    • 10.1373/clinchem.2004.031336, 15229160
    • Spiess AN, Mueller N, Ivell R. Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of Taq polymerase by the disaccharide trehalose. Clin Chem 2004, 50(7):1256-9. 10.1373/clinchem.2004.031336, 15229160.
    • (2004) Clin Chem , vol.50 , Issue.7 , pp. 1256-1259
    • Spiess, A.N.1    Mueller, N.2    Ivell, R.3
  • 19
    • 0030001524 scopus 로고    scopus 로고
    • Dimethyl sulfoxide improves RNA amplification
    • Sidhu MK, Liao MJ, Rashidbaigi A. Dimethyl sulfoxide improves RNA amplification. BioTechniques 1996, 21(1):44-7.
    • (1996) BioTechniques , vol.21 , Issue.1 , pp. 44-47
    • Sidhu, M.K.1    Liao, M.J.2    Rashidbaigi, A.3
  • 20
    • 0035865110 scopus 로고    scopus 로고
    • Dimethyl sulfoxide as additive in ready-to-use reaction mixtures for real-time polymerase chain reaction analysis with SYBR Green I dye
    • 10.1006/abio.2000.4931, 11161325
    • Jung M, Muche JM, Lukowsky A, Jung K, Loening SA. Dimethyl sulfoxide as additive in ready-to-use reaction mixtures for real-time polymerase chain reaction analysis with SYBR Green I dye. Anal Biochem 2001, 289(2):292-5. 10.1006/abio.2000.4931, 11161325.
    • (2001) Anal Biochem , vol.289 , Issue.2 , pp. 292-295
    • Jung, M.1    Muche, J.M.2    Lukowsky, A.3    Jung, K.4    Loening, S.A.5
  • 21
    • 0029752741 scopus 로고    scopus 로고
    • Strategy for controlling preferential amplification and avoiding false negatives in PCR typing
    • Weissensteiner T, Lanchbury JS. Strategy for controlling preferential amplification and avoiding false negatives in PCR typing. BioTechniques 1996, 21(6):1102-8.
    • (1996) BioTechniques , vol.21 , Issue.6 , pp. 1102-1108
    • Weissensteiner, T.1    Lanchbury, J.S.2
  • 22
    • 0030883816 scopus 로고    scopus 로고
    • Betaine improves the PCR amplification of GC-rich DNA sequences
    • 10.1093/nar/25.19.3957, 146979, 9380524
    • Henke W, Herdel K, Jung K, Schnorr D, Loening SA. Betaine improves the PCR amplification of GC-rich DNA sequences. Nucleic Acids Res 1997, 25(19):3957-8. 10.1093/nar/25.19.3957, 146979, 9380524.
    • (1997) Nucleic Acids Res , vol.25 , Issue.19 , pp. 3957-3958
    • Henke, W.1    Herdel, K.2    Jung, K.3    Schnorr, D.4    Loening, S.A.5
  • 23
    • 0029820375 scopus 로고    scopus 로고
    • Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content
    • 10.1101/gr.6.7.633, 8796351
    • Baskaran N, Kandpal RP, Bhargava AK, Glynn MW, Bale A, Weissman SM. Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. Genome Res 1996, 6(7):633-8. 10.1101/gr.6.7.633, 8796351.
    • (1996) Genome Res , vol.6 , Issue.7 , pp. 633-638
    • Baskaran, N.1    Kandpal, R.P.2    Bhargava, A.K.3    Glynn, M.W.4    Bale, A.5    Weissman, S.M.6
  • 24
    • 77954069646 scopus 로고    scopus 로고
    • Enhanced amplification of GC-rich DNA with two organic reagents
    • 10.2144/000113203, 19852763
    • Zhang Z, Yang X, Meng L, Liu F, Shen C, Yang W. Enhanced amplification of GC-rich DNA with two organic reagents. BioTechniques 2009, 47(3):775-9. 10.2144/000113203, 19852763.
    • (2009) BioTechniques , vol.47 , Issue.3 , pp. 775-779
    • Zhang, Z.1    Yang, X.2    Meng, L.3    Liu, F.4    Shen, C.5    Yang, W.6
  • 25
    • 0033844637 scopus 로고    scopus 로고
    • Improved cycle sequencing of GC-rich templates by a combination of nucleotide analogs
    • Motz M, Paabo S, Kilger C. Improved cycle sequencing of GC-rich templates by a combination of nucleotide analogs. BioTechniques 2000, 29(2):268-70.
    • (2000) BioTechniques , vol.29 , Issue.2 , pp. 268-270
    • Motz, M.1    Paabo, S.2    Kilger, C.3
  • 26
    • 33751165548 scopus 로고    scopus 로고
    • Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences
    • 10.2353/jmoldx.2006.060058, 1876170, 17065422
    • Musso M, Bocciardi R, Parodi S, Ravazzolo R, Ceccherini I. Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences. J Mol Diagn 2006, 8(5):544-50. 10.2353/jmoldx.2006.060058, 1876170, 17065422.
    • (2006) J Mol Diagn , vol.8 , Issue.5 , pp. 544-550
    • Musso, M.1    Bocciardi, R.2    Parodi, S.3    Ravazzolo, R.4    Ceccherini, I.5
  • 27
    • 77649113149 scopus 로고    scopus 로고
    • Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of taq
    • 10.2353/jmoldx.2010.090070, 2871721, 20075207
    • Zhang Z, Kermekchiev MB, Barnes WM. Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of taq. J Mol Diagn 2010, 12(2):152-61. 10.2353/jmoldx.2010.090070, 2871721, 20075207.
    • (2010) J Mol Diagn , vol.12 , Issue.2 , pp. 152-161
    • Zhang, Z.1    Kermekchiev, M.B.2    Barnes, W.M.3
  • 28
    • 0025743732 scopus 로고
    • 'Touchdown' PCR to circumvent spurious priming during gene amplification
    • 10.1093/nar/19.14.4008, 328507, 1861999
    • Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 1991, 19(14):4008. 10.1093/nar/19.14.4008, 328507, 1861999.
    • (1991) Nucleic Acids Res , vol.19 , Issue.14 , pp. 4008
    • Don, R.H.1    Cox, P.T.2    Wainwright, B.J.3    Baker, K.4    Mattick, J.S.5
  • 29
    • 23944509929 scopus 로고    scopus 로고
    • Improved PCR method for amplification of GC-rich DNA sequences
    • Hubé F, Reverdiau P, Iochmann S, Gruel Y. Improved PCR method for amplification of GC-rich DNA sequences. Mol Biotechnol 2005, 31(1):81-4.
    • (2005) Mol Biotechnol , vol.31 , Issue.1 , pp. 81-84
    • Hubé, F.1    Reverdiau, P.2    Iochmann, S.3    Gruel, Y.4
  • 30
    • 63249099357 scopus 로고    scopus 로고
    • Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples
    • 10.1093/nar/gkn1055, 2655666, 19208643
    • Kermekchiev MB, Kirilova LI, Vail EE, Barnes WM. Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples. Nucleic Acids Res 2009, 37(5):e40. 10.1093/nar/gkn1055, 2655666, 19208643.
    • (2009) Nucleic Acids Res , vol.37 , Issue.5
    • Kermekchiev, M.B.1    Kirilova, L.I.2    Vail, E.E.3    Barnes, W.M.4
  • 31
    • 43249095784 scopus 로고    scopus 로고
    • Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations
    • 10.1021/bi702363u, 18422348
    • Owczarzy R, Moreira BG, You Y, Behlke MA, Walder JA. Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations. Biochemistry 2008, 47(19):5336-53. 10.1021/bi702363u, 18422348.
    • (2008) Biochemistry , vol.47 , Issue.19 , pp. 5336-5353
    • Owczarzy, R.1    Moreira, B.G.2    You, Y.3    Behlke, M.A.4    Walder, J.A.5
  • 32
    • 0032528128 scopus 로고    scopus 로고
    • Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR
    • 10.1093/nar/26.14.3340, 147702, 9649616
    • McDowell DG, Burns NA, Parkes HC. Localised sequence regions possessing high melting temperatures prevent the amplification of a DNA mimic in competitive PCR. Nucleic Acids Res 1998, 26(14):3340-7. 10.1093/nar/26.14.3340, 147702, 9649616.
    • (1998) Nucleic Acids Res , vol.26 , Issue.14 , pp. 3340-3347
    • McDowell, D.G.1    Burns, N.A.2    Parkes, H.C.3
  • 33
    • 0031905770 scopus 로고    scopus 로고
    • Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA
    • 10.1073/pnas.95.2.520, 18452, 9435224
    • Carninci P, Nishiyama Y, Westover A, Itoh M, Nagaoka S, Sasaki N, Okazaki Y, Muramatsu M, Hayashizaki Y. Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA. Proc Natl Acad Sci USA 1998, 95(2):520-4. 10.1073/pnas.95.2.520, 18452, 9435224.
    • (1998) Proc Natl Acad Sci USA , vol.95 , Issue.2 , pp. 520-524
    • Carninci, P.1    Nishiyama, Y.2    Westover, A.3    Itoh, M.4    Nagaoka, S.5    Sasaki, N.6    Okazaki, Y.7    Muramatsu, M.8    Hayashizaki, Y.9
  • 34
    • 79957459823 scopus 로고    scopus 로고
    • Chemical PCR: Composition for enhancing polynucleotide amplification reactions
    • Chakrabarti R, Schutt C. Chemical PCR: Composition for enhancing polynucleotide amplification reactions. US patent 7772383 2004,
    • (2004) US patent 7772383
    • Chakrabarti, R.1    Schutt, C.2
  • 35
    • 0034219149 scopus 로고    scopus 로고
    • New specificity and yield enhancer of polymerase chain reactions
    • 102717, 10871414
    • Kovárová M, Dráber P. New specificity and yield enhancer of polymerase chain reactions. Nucleic Acids Res 2000, 28(13):e70. 102717, 10871414.
    • (2000) Nucleic Acids Res , vol.28 , Issue.13
    • Kovárová, M.1    Dráber, P.2
  • 37
    • 0002463562 scopus 로고
    • Purifying and Analyzing Genomic DNA
    • Cold Spring Harbor, New York, NY, Cold Spring Harbor Laboratory Press, Birren B, Green ED, Klapholz S, Myers RM, Roskams J
    • Wolff R, Gemmill R. Purifying and Analyzing Genomic DNA. Genome Analysis: A Laboratory Manual 1977, 1-81. Cold Spring Harbor, New York, NY, Cold Spring Harbor Laboratory Press, Birren B, Green ED, Klapholz S, Myers RM, Roskams J.
    • (1977) Genome Analysis: A Laboratory Manual , pp. 1-81
    • Wolff, R.1    Gemmill, R.2
  • 38
    • 0003535927 scopus 로고    scopus 로고
    • Animal Genome Size Database
    • Gregory TR. Animal Genome Size Database. 2010, http://www.genomesize.com
    • (2010)
    • Gregory, T.R.1
  • 40
    • 0005865661 scopus 로고    scopus 로고
    • Measuring the fidelity of Platinum Pfx DNA polymerase
    • Lackovich KK, Lee JE, Chang P, Rashtchian A. Measuring the fidelity of Platinum Pfx DNA polymerase. Focus 2001, 23:6-7.
    • (2001) Focus , vol.23 , pp. 6-7
    • Lackovich, K.K.1    Lee, J.E.2    Chang, P.3    Rashtchian, A.4
  • 41
    • 0028038072 scopus 로고
    • Monoclonal antibodies prepared against the DNA polymerase from Thermus aquaticus are potent inhibitors of enzyme activity
    • 10.1016/0022-1759(94)90102-3, 7518481
    • Scalice ER, Sharkey DJ, Daiss JL. Monoclonal antibodies prepared against the DNA polymerase from Thermus aquaticus are potent inhibitors of enzyme activity. J Immunol Methods 1994, 172(2):147-63. 10.1016/0022-1759(94)90102-3, 7518481.
    • (1994) J Immunol Methods , vol.172 , Issue.2 , pp. 147-163
    • Scalice, E.R.1    Sharkey, D.J.2    Daiss, J.L.3
  • 42
    • 0000899673 scopus 로고
    • Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme
    • Aposhian HV, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme. J Biol Chem 1962, 237(2):519-25.
    • (1962) J Biol Chem , vol.237 , Issue.2 , pp. 519-525
    • Aposhian, H.V.1    Kornberg, A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.