Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA

Proceedings of the National Academy of Sciences of the United States of America

Volumn 95, Issue 2, 1998, Pages 520-524

  Carninci, Piero ^a   Nishiyama, Yoko ^a   Westover, Arthur ^a   Itoh, Masayoshi ^a   Nagaoka, Sumiharu ^a   Sasaki, Nobuya ^a   Okazaki, Yasushi ^a   Muramatsu, Masami ^a   Hayashizaki, Yoshihide ^a,b  

^a RIKEN TSUKUBA INSTITUTE   (Japan)

^b University of Tsukuba School of Medicine   (Japan)

Author keywords

Chaperonin like molecules; Enzyme thermostability; Polyols; Reverse transcriptase

Indexed keywords

COMPLEMENTARY DNA; DEOXYRIBONUCLEASE I; LIGASE; TREHALOSE;

ARTICLE; DNA SYNTHESIS; ENZYME STABILITY; HEAT SENSITIVITY; MOLONEY LEUKEMIA ONCOVIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SACCHAROMYCES CEREVISIAE; THERMOPHILIC BACTERIUM; THERMOREGULATION; THERMOSTABILITY;

DNA, COMPLEMENTARY; ENZYME STABILITY; LEUKEMIA VIRUS, MURINE; RNA-DIRECTED DNA POLYMERASE; TEMPERATURE; TREHALOSE;

BACTERIA (MICROORGANISMS); MOLONEY MURINE LEUKEMIA VIRUS; ONCOVIRINAE; SACCHAROMYCES CEREVISIAE;

EID: 0031905770     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.95.2.520     Document Type: Article

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