Enhanced amplification of GC-rich DNA with two organic reagents

BioTechniques

Volumn 47, Issue 3, 2009, Pages 775-779

  Zhang, Zhizhou ^a,b   Yang, Xia ^a,b   Meng, Liangyu ^b   Liu, Fang ^b   Shen, Cenchao ^a,b   Yang, Wenjuan ^a,b  

^a HARBIN INSTITUTE OF TECHNOLOGY   (China)

^b TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

1,2 Propanediol; Ethylene glycol; GC rich DNA; PCR

Indexed keywords

BETAINE; DNA POLYMERASE; ETHYLENE GLYCOL; PROPYLENE GLYCOL;

AMPLICON; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; GC RICH SEQUENCE; GENE AMPLIFICATION; HUMAN; POLYMERASE CHAIN REACTION; NUCLEIC ACID AMPLIFICATION; PROCEDURES;

ETHYLENE GLYCOL; GC RICH SEQUENCE; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PROPYLENE GLYCOL; SEQUENCE ANALYSIS, DNA;

EID: 77954069646     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113203     Document Type: Article

References (13)

1. Weissensteiner, T. and J.S. Lanchbury. 1996. Strategy for controlling preferential amplification and avoiding false negatives in PCR typing. Biotechniques 21:1102-1108. (Pubitemid 26422018)
2. Sahdev, S., S. Saini, P. Tiwari, S. Saxena, and K. Singh Saini. 2007. Amplification of GC-rich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions. Mol. Cell. Probes 21:303-307.
3. Henke, W., K. Herdel, K. Jung, D. Schnorr, and S.A. Loening. 1997. Betaine improves the PCR amplification of GC-rich DNA sequences. Nucleic Acids Res. 25:3957-3958.
4. Musso, M., R. Bocciardi, S. Parodi, R. Ravazzolo, and I. Ceccherini. 2006. Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences. J. Mol. Diagn. 8:544-550.
5. Kang, J., M.S. Lee, and D.G. Gorenstein. 2005. The enhancement of PCR amplification of a random sequence DNA library by DMSO and betaine: application to in vitro combinatorial selection of aptamers. J. Biochem. Biophys. Methods 64:147-151.
6. Turner, S.L. and F.J. Jenkins. 1995. Use of deoxyinosine in PCR to improve amplification of GC-rich DNA. Biotechniques 19:48-52.
7. Frey, U.H., H.S. Bachmann, J. Peters, and W. Siffert. 2008. PCR-amplification of GC-rich regions: 'slowdown PCR'. Nat. Protocols 3:1312-1317.
8. Mamedov, T.G., E. Pienaar, S.E. Whitney, J.R. TerMaat, G. Carvill, R. Goliath, A. Subramanian, and H.J. Viljoen. 2008. A fundamental study of the PCR amplification of GC-rich DNA templates. Comput. Biol. Chem. 32:452-457.
9. Benita, Y., R.S. Oosting, M.C. Lok, M.J. Wise, and I. Humphery-Smith. 2003. Regionalized GC content of template DNA as a predictor of PCR success. Nucleic Acids Res. 31:e99.
10. Fujii, S., M. Akiyama, K. Aoki, Y. Sugaya, K. Higuchi, M. Hiraoka, Y. Miki, N. Saitoh, et al. 1999. DNA replication errors produced by the replicative apparatus of Escherichia coli. J. Mol. Biol. 289:835-850.
11. Spink, C.H., N. Garbett, and J.B. Chaires. 2007. Enthalpies of DNA melting in the presence of osmolytes. Biophys. Chem. 126:176-185.
12. Nords t rom, L. J . , C. A. Cla rk, B. Andersen, S.M. Champlin, and J.J. Schwinefus. 2006. Effect of ethylene glycol, urea, and N-methylated glycines on DNA thermal stability: the role of DNA base pair composition and hydration. Biochemistry 45:9604-9614.
13. Schwartz, A.M. and G.D. Fasman. 1979. Thermal denaturation of chromatin and lysine copolymer-DNA complexes. Effects of ethylene glycol. Biopolymers 18:1045-1063.