Trehalose is a potent PCR enhancer: Lowering of DNA melting temperature and thermal stabilization of taq polymerase by the disaccharide trehalose

Clinical Chemistry

Volumn 50, Issue 7, 2004, Pages 1256-1259

  Spiess, Andrej Nikolai ^a   Mueller, Nadine ^a   Ivell, Richard ^a  

^a Institut fur Hormon und Fortpflanzungsforschung IHF   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BETAINE; OXYTOCIN RECEPTOR; TREHALOSE;

ANIMAL TISSUE; ARTICLE; CONCENTRATION (PARAMETERS); DNA DENATURATION; ENHANCER REGION; GENETIC TRANSCRIPTION; MEDICAL INSTRUMENTATION; MELTING POINT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; THERMOSTABILITY;

ANIMALS; BRAIN CHEMISTRY; DISACCHARIDES; DNA; ENZYME STABILITY; HEATING; MICE; RECEPTORS, OXYTOCIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TAQ POLYMERASE; TRANSITION TEMPERATURE; TREHALOSE;

ANIMALIA;

EID: 3042614052     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2004.031336     Document Type: Article

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