Radiation damage in protein crystals is reduced with a micron-sized X-ray beam

Proceedings of the National Academy of Sciences of the United States of America

Volumn 108, Issue 15, 2011, Pages 6127-6132

  Sanishvili, Ruslan ^a   Yoder, Derek W ^a   Pothineni, Sudhir Babu ^a   Rosenbaum, Gerd ^b   Xu, Shenglan ^a   Vogt, Stefan ^a   Stepanov, Sergey ^a   Makarov, Oleg A ^a   Corcoran, Stephen ^a   Benn, Richard ^a   Nagarajan, Venugopalan ^a   Smith, Janet L ^a,c   Fischetti, Robert F ^a  

^a ARGONNE NATIONAL LABORATORY   (United States)

^b University of Georgia ^*  (United States)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Microcrystallography; Synchrotron radiation

Indexed keywords

ANISOTROPY; CONFERENCE PAPER; ENERGY TRANSFER; POLARIZATION; PRIORITY JOURNAL; RADIATION DOSE; RADIATION INJURY; SIMULATION; SPECTROSCOPY; X RAY CRYSTALLOGRAPHY; X RAY DIFFRACTION; X RAY PHOTOELECTRON SPECTROSCOPY;

EID: 79955043510     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1017701108     Document Type: Article

References (35)

1. Nelson R, et al. (2005) Structure of the cross-beta spine of amyloid-like fibrils. Nature 435:773-778. (Pubitemid 40839722)
2. Holton JM, Frankel KA (2010) The minimum crystal size needed for a complete diffraction data set. Acta Crystallogr D 66:393-408.
3. Moukhametzianov R, et al. (2008) Protein crystallography with a micrometre-sized synchrotron-radiation beam. Acta Crystallogr D 64:158-166. (Pubitemid 351161591)
4. Sanishvili R, et al. (2008) A 7 ìm mini-beam improves diffraction data from small or imperfect crystals of macromolecules. Acta Crystallogr D 64:425-435. (Pubitemid 351507805)
5. O'Neill P, Stevens DL, Garman EF (2002) Physical and chemical considerations of damage induced in protein crystals by synchrotron radiation: A radiation chemical perspective. J Synchrotron Radiat 9:329-332.
6. Murray J, Garman E (2002) Investigation of possible free-radical scavengers and metrics for radiation damage in protein cryocrystallography. J Synchrotron Radiat 9:347-354.
7. Diederichs K, McSweeney S, Ravelli RB (2003) Zero-dose extrapolation as part of macromolecular synchrotron data reduction. Acta Crystallogr D 59:903-909. (Pubitemid 36599370)
8. Diederichs K (2006) Some aspects of quantitative analysis and correction of radiation damage. Acta Crystallogr D 62:96-101.
9. Wang J, Ealick SE (2004) Observation of time-resolved structural changes by linear interpolation of highly redundant X-ray diffraction data. Acta Crystallogr D 60:1579-1585. (Pubitemid 41103530)
10. Ravelli RB, Leiros HK, Pan B, CaffreyM, McSweeney S (2003) Specific radiation damage can be used to solve macromolecular crystal structures. Structure 11:217-224. (Pubitemid 36183645)
11. Ravelli RB, Nanao MH, Lovering A, White S, McSweeney S (2005) Phasing in the presence of radiation damage. J Synchrotron Radiat 12:276-284. (Pubitemid 41557604)
12. Nanao MH, Sheldrick GM, Ravelli RB (2005) Improving radiation-damage substructures for RIP. Acta Crystallogr D 61:1227-1237. (Pubitemid 43964541)
13. Evans G, Polentarutti M, Djinovic Carugo K, Bricogne G (2003) SAD phasing with triiodide, softer X-rays and some help from radiation damage. Acta Crystallogr D 59:1429-1434. (Pubitemid 36978306)
14. Banumathi S, Zwart PH, Ramagopal UA, Dauter M, Dauter Z (2004) Structural effects of radiation damage and its potential for phasing. Acta Crystallogr D 60:1085-1093. (Pubitemid 41295448)
15. Ramagopal UA, Dauter Z, Thirumuruhan R, Fedorov E, Almo SC (2005) Radiationinduced site-specific damage of mercury derivatives: Phasing and implications. Acta Crystallogr D 61:1289-1298. (Pubitemid 43964543)
16. Schiltz M, Bricogne G (2007) Modelling and refining site-specific radiation damage in SAD/MAD phasing. J Synchrotron Radiat 14:34-42. (Pubitemid 46058904)
17. Smith NV, Himpsel FJ, Eastman DE, Farge Y, eds. (1983) Handbook on Synchrotron Radiation (North-Holland, New York), 1b, pp 917-921.
18. Nave C, Hill MA (2005) Will reduced radiation damage occur with very small crystals? J Synchrotron Radiat 12:299-303.
19. Cowan JA, Nave C (2008) The optimum conditions to collect X-ray data from very small samples. J Synchrotron Radiat 15:458-462.
20. Fischetti RF, et al. (2009) Mini-beam collimator enables micro-crystallography experiments on standard beamlines. J Synchrotron Radiat 16:217-225.
21. Flot D, et al. (2010) The ID23-2 structural biology microfocus beamline at the ESRF. J Synchrotron Radiat 17:107-118.
22. Riekel C (2004) Recent developments in microdiffraction on protein crystals. J Synchrotron Radiat 11:4-6. (Pubitemid 40085620)
23. Thomas SJ (1994) Factors affecting penumbral shape and 3D dose distributions in stereotactic radiotherapy. Phys Med Biol 39:761-771. (Pubitemid 24118798)
24. Paithankar KS, Owen RL, Garman EF (2009) Absorbed dose calculations for macromolecular crystals: Improvements to RADDOSE. J Synchrotron Radiat 16:152-162.
25. Owen RL, Rudino-Pinera E, Garman EF (2006) Experimental determination of the radiation dose limit for cryocooled protein crystals. Proc Natl Acad Sci USA 103:4912-4917.
26. Riekel C, Burghammer M, Schertler G (2005) Protein crystallography microdiffraction. Curr Opin Struct Biol 15:556-562. (Pubitemid 41393487)
27. Glaeser R, et al. (2000) Characterization of conditions required for X-Ray diffraction experiments with protein microcrystals. Biophys J 78:3178-3185. (Pubitemid 30396946)
28. Boutet S, Robinson IK (2006) Radiation driven collapse of protein crystals. J Synchrotron Radiat 13:1-7.
29. Rasmussen SG, et al. (2007) Crystal structure of the human beta2 adrenergic G-proteincoupled receptor. Nature 450:383-387. (Pubitemid 350126755)
30. Finfrock YZ, et al. (2010) Spatial dependence and mitigation of radiation damage by a line-focus mini-beam. Acta Crystallogr D 66:1287-1294.
31. Stern EA, et al. (2009) Reducing radiation damage in macromolecular crystals at synchrotron sources. Acta Crystallogr D 65:366-374.
32. Paithankar KS, Garman EF (2010) Know your dose: RADDOSE. Acta Crystallogr D 66:381-388.
33. Fischetti RF, et al. (2007) Optical performance of the GM/CA-CAT canted undulator beamlines for protein crystallography. AIP Conference Proceedings: Ninth International Conference on Synchrotron Radiation Instrumentation, eds J-Y Choi and S Rah 879 (Springer, New York), pp 754-757.
34. Kirz J, Jacobsen C, Howells M (1999) Soft X-ray microscopes and their biological applications. Q Rev Biophys 28:33-130.
35. Xu S, et al. (2010) Micro-crystallography developments at GM/CA-CAT at the APS. AIP Conference Proceedings: Tenth International Conference on Synchrotron Radiation Instrumentation, 1234 (Springer, New York), pp 905-908.