Multiplexed protein quantification in maize leaves by liquid chromatography coupled with tandem mass spectrometry: An alternative tool to immunoassays for target protein analysis in genetically engineered crops

Journal of Agricultural and Food Chemistry

Volumn 59, Issue 8, 2011, Pages 3551-3558

  Hu, X Tiger ^a   Owens, Michaela A ^a  

^a PIONEER HI BRED INTERNATIONAL INC   (United States)

Author keywords

Liquid chromatography; mass spectrometry; multiplex; protein quantification; transgene analysis

Indexed keywords

CORRELATION COEFFICIENT; CRUDE EXTRACT; GENETICALLY ENGINEERED CROPS; INFORMATION-DEPENDENT ACQUISITIONS; LC-MS/MS; LINEAR DYNAMICS; MICROWAVE-ASSISTED; MULTIPLE REACTION MONITORING; MULTIPLEX; ORDERS OF MAGNITUDE; PROTEIN QUANTIFICATION; RECOMBINANT PROTEIN; REPRODUCIBILITIES; TANDEM MASS SPECTROMETRY; TARGET PROTEINS; TRANSGENE; TRYPSIN DIGESTION;

COLUMN CHROMATOGRAPHY; CROPS; GENETIC ENGINEERING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MULTIPLEXING; OPTIMIZATION; PEPTIDES;

LIQUIDS;

TRYPSIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CALIBRATION; CHEMISTRY; CROP; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOASSAY; MAIZE; METHODOLOGY; MOLECULAR GENETICS; PLANT LEAF; QUALITY CONTROL; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; CALIBRATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROPS, AGRICULTURAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOASSAY; MOLECULAR SEQUENCE DATA; PLANT LEAVES; PLANT PROTEINS; QUALITY CONTROL; TANDEM MASS SPECTROMETRY; TRYPSIN; ZEA MAYS;

ZEA MAYS;

EID: 79955029862     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf104516r     Document Type: Article

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