Production of Candida antarctica lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in an ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production

Journal of Laboratory Automation

Volumn 16, Issue 1, 2011, Pages 17-37

  Hughes, Stephen R ^a   Moser, Bryan R ^a   Harmsen, Amanda J ^b,c   Bischoff, Kenneth M ^a   Jones, Marjorie A ^b   Pinkelman, Rebecca ^d   Bang, Sookie S ^d   Tasaki, Ken ^c   Doll, Kenneth M ^a   Qureshi, Nasib ^a   Saha, Badal C ^a   Liu, Siqing ^a   Jackson, John S ^a   Robinson, Samantha ^b   Cotta, Michael C ^a   Rich, Joseph O ^a   Caimi, Paolo ^e  

^a NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH   (United States)

^b ILLINOIS STATE UNIVERSITY   (United States)

^c MITSUBISHI CHEMICAL CORPORATION   (Japan)

^d South Dakota School of Mines and Technology   (United States)

^e Resindion Srl   (Italy)

Author keywords

Biodiesel; Candida antarctica lipase B; One stepcharging resin; Resin supported biocatalyst; Saccharomyces cerevisiae; Transesterification

Indexed keywords

BIOCATALYSTS; BIODIESEL; BIOETHANOL; CANDIDA; CARBON; CATALYSIS; ENZYMES; ESTERS; ETHANOL; GENE EXPRESSION; GENES; ISOMERS; PROTEINS; RESINS; ROBOTIC ASSEMBLY; ROBOTICS; SOYBEAN OIL; TRANSESTERIFICATION; YEAST;

BIO-ETHANOL PRODUCTION; BIODIESEL PRODUCTION; CANDIDA ANTARCTICA LIPASE B; CANDIDA ANTARCTICA LIPASEB (CALB); FATTY ACID ETHYL ESTER; HIGH LEVEL EXPRESSION; INTEGRATED BIOREFINERY; SACCHAROMYCES CEREVISIAE STRAINS;

FATTY ACIDS;

ALCOHOL; BIODIESEL; CORN OIL; FATTY ACID ESTER; GALACTOSE; GLUCOSE; LIPASE B; LYCOTOXIN 1; MYCOTOXIN; RESIN; SOYBEAN OIL; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; BIOCATALYST; BIOFUEL PRODUCTION; CANDIDA ANTARCTICA; CARBON SOURCE; CELL SURFACE; CONTROLLED STUDY; DNA PURIFICATION; ENZYME LOCALIZATION; FERMENTATION MEDIUM; GENE EXPRESSION; GENETIC VARIABILITY; LABORATORY AUTOMATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN ASSEMBLY; ROBOTICS; UPREGULATION; YEAST CELL;

EID: 79955019180     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1016/j.jala.2010.04.002     Document Type: Article

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