Cost-Effective High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System

JALA - Journal of the Association for Laboratory Automation

Volumn 12, Issue 4, 2007, Pages 202-212

  Hughes, Stephen R ^a   Dowd, Patrick F ^a   Hector, Ronald E ^a   Riedmuller, Steven B ^b   Bartolett, Scott ^b   Mertens, Jeffrey A ^a   Qureshi, Nasib ^a   Liu, Siqing ^a   Bischoff, Kenneth M ^a   Li, Xin Liang ^a   Jackson Jr , John S ^a   Sterner, David ^c   Panavas, Tadas ^c   Rich, Joseph O ^a   Farrelly, Philip J ^b   Butt, Tauseef ^c   Cotta, Michael A ^a  

^a NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH   (United States)

^b Hudson Control Group Inc   (United States)

^c LifeSensors Inc   (United States)

Author keywords

automated amino acid scanning mutagenesis (AASM) library construction; automated vacuum block; cost effective fully automated high throughput molecular biology; large scale optimized open reading frame cloning and bacterial transformation

Indexed keywords

AUTOMATED MOLECULAR BIOLOGY; AUTOMATED VACUUM BLOCK;

BACTERIOLOGY; BIOLOGY; COST EFFECTIVENESS; DIGITAL LIBRARIES; GENES;

ROBOTICS;

INSECT VENOM; INSECTICIDE; LYCOTOXIN 1; PEPTIDE; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; AUTOMATION; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM TRANSFORMATION; COLONY FORMING UNIT; CONTROLLED STUDY; COST CONTROL; ESCHERICHIA COLI; EXPRESSION VECTOR; FILTRATION; FUNCTIONAL PROTEOMICS; GENE EXPRESSION; GENE LIBRARY; GENE MUTATION; GENE SEQUENCE; HIGH THROUGHPUT SCREENING; MOLECULAR BIOLOGY; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; PROCESS CONTROL; PROTEIN ASSEMBLY; PROTEIN PURIFICATION; ROBOTICS; VACUUM;

EID: 34447279461     PISSN: 15355535     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2007.04.007     Document Type: Article

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