Preparation of a whole-cell biocatalyst of mutated Candida antarctica lipase B (mCALB) by a yeast molecular display system and its practical properties

Applied Microbiology and Biotechnology

Volumn 75, Issue 3, 2007, Pages 549-555

  Kato, Michiko ^a   Fuchimoto, Jun ^a   Tanino, Takanori ^b   Kondo, Akihiko ^b   Fukuda, Hideki ^b   Ueda, Mitsuyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

Candida antarctica lipase B; Mutation; Substrate specificity; Yeast cell surface engineering

Indexed keywords

CANDIDA ANTARCTICA LIPASE B; MUTATION; SUBSTRATE SPECIFICITY; YEAST CELL SURFACE ENGINEERING;

CELLS; GENETIC ENGINEERING; MOLECULAR STRUCTURE; THERMAL EFFECTS;

BIOCATALYSTS;

4 NITROPHENYLBUTYRATE; AGGLUTININ; BUTYRIC ACID DERIVATIVE; FUNGAL ENZYME; INDUSTRIAL ENZYME; LIPASE B; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

CATALYSIS; ENZYME ACTIVITY; FATTY ACID; GENETIC ENGINEERING; MUTATION; PROTEIN; SUBSTRATE; THERMODYNAMICS; YEAST;

AMINO ACID SEQUENCE; ARTICLE; BIOCATALYST; CANDIDA ANTARCTICA; CELL SURFACE; CONTROLLED STUDY; DISPLAY SYSTEM; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; FUNGAL STRAIN; FUNGUS MUTATION; HEAT TREATMENT; NONHUMAN; NUCLEOTIDE SEQUENCE; RHIZOPUS ORYZAE; THERMOSTABILITY; WHOLE CELL; YEAST CELL;

CANDIDA; CELL WALL; ENZYME STABILITY; LIPASE; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; MUTATION; RHIZOPUS; SUBSTRATE SPECIFICITY; TEMPERATURE;

CANDIDA ANTARCTICA; RHIZOPUS ORYZAE;

EID: 34248672267     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0835-2     Document Type: Article

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