Perilipin 1 and perilipin 2 protein localization and gene expression study in skeletal muscles of European cross-breed pigs with different intramuscular fat contents

Meat Science

Volumn 88, Issue 4, 2011, Pages 631-637

  Gandolfi, G ^a   Mazzoni, M ^a   Zambonelli, P ^a   Lalatta Costerbosa, G ^a   Tronca, A ^a   Russo, V ^a   Davoli, R ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Gene expression; Immunofluorescence; Intramuscular fat; Meat quality; Perilipin; Pigs

Indexed keywords

IMMUNOFLUORESCENCE; INTRAMUSCULAR FAT; MEAT QUALITY; PERILIPIN; PIGS;

FLUORESCENCE; LIPIDS; MAMMALS; MUSCLE; PROTEINS;

GENE EXPRESSION;

SUIDAE;

FAT; MESSENGER RNA; PERILIPIN; PHOSPHOPROTEIN;

ADIPOCYTE; ANIMAL; ARTICLE; EUROPE; GENE EXPRESSION; GENETICS; HYBRIDIZATION; ISOLATION AND PURIFICATION; MALE; METABOLISM; SKELETAL MUSCLE; SWINE; WESTERN BLOTTING;

ADIPOCYTES; ANIMALS; BLOTTING, WESTERN; EUROPE; FATS; GENE EXPRESSION; HYBRIDIZATION, GENETIC; MALE; MUSCLE, SKELETAL; PHOSPHOPROTEINS; RNA, MESSENGER; SWINE;

EID: 79955000136     PISSN: 03091740     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.meatsci.2011.02.020     Document Type: Article

References (39)

1. Official method 991.36. Fat (crude) in meat and meat products. Journal of AOAC International 1992, 75:292. AOAC Association of Official Analytical Chemists.
2. Bickel P.E., Tansey J.T., Welte M.A. PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores. Biochimica et Biophysica Acta 2009, 6:419-440.
3. Brasaemle D.L., Barber T., Kimmel A.R., Londos C. Post-translational regulation of perilipin expression. Journal of Biological Chemistry 1997, 272:9378-9387.
4. Brooke M.H., Kaiser K.K. Muscle fiber types: how many and what kind?. Archives of Neurology 1970, 23:369-379.
5. Bustin S.A. A-Z of quantitative PCR 2004, International University Line Press, La Jolla, CA.
6. Damon M., Louveau I., Lefaucheur L., Lebret B., Vincent A., Leroy P., et al. Number of intramuscular adipocytes and fatty acid binding protein-4 content are significant indicators of intramuscular fat level in crossbred Large White×Duroc pigs. Journal of Animal Science 2006, 84:1083-1092.
7. Davoli R., Gandolfi G., Braglia S., Comella M., Zambonelli P., Buttazzoni L., Russo V. New SNP of the porcine Perilipin 2 (PLIN2) gene, association with carcass traits and expression analysis in skeletal muscle. Molecular Biology Report 2011, 38:1575-1583.
8. Deram S., Nicolau C.Y., Perez-Martinez P., Guazzelli I., Halpern A., Wajchenberg B.L., et al. Effects of perilipin (PLIN) gene variation on metabolic syndrome risk and weight loss in obese children and adolescents. Journal of Clinical Endocrinology & Metabolism 2008, 93:4933-4940.
9. Dyck D.J., Peters S.J., Glatz J., Gorski J., Keizer H., Kiens B., et al. Functional differences in lipid metabolism in resting skeletal muscle of various fiber types. American Journal of Physiology 1997, 272:E340-E351.
10. Essen B., Jansson E., Henriksson J., Taylor A.W., Saltin B. Metabolic characteristics of fibre types in human skeletal muscle. Acta Physiologica 1975, 95:153-165.
11. Essén-Gustavsson B., Karlsson A., Lundstrom K., Enfält A.C. Intramuscular fat and muscle fibre lipid contents in halothane-gene-free pigs fed high or low protein diets and its relation to meat quality. Meat Science 1994, 38:269-277.
12. Fernandez X., Monin G., Talmant A., Mourot J., Lebret B. Influence of intramuscular fat content on the quality of pig meat - 1. Composition of the lipid fraction and sensory characteristics of m. longissimus lumborum. Meat Science 1999, 53:59-65.
13. Fernandez X., Mourot J., Lebret B., Gilbert S., Monin G. Influence of intramuscular fat content on lipid composition, sensory qualities and consumer acceptability of cured cooked ham. Journal of the Science of Food and Agriculture 2000, 80:705-710.
14. Gao S.Z., Zhao S.M. Physiology, affecting factors and strategies for control of pig meat intramuscular fat. Recent Patents in Food, Nutrition & Agriculture 2009, 1:59-74.
15. Gondret F., Lebret B. Feeding intensity and dietary protein level affect adipocyte cellularity and lipogenic capacity of muscle homogenates in growing pigs, without modification of the expression of sterol regulatory element binding protein. Journal of Animal Science 2002, 80:3184-3193.
16. Guillerm-Regost C., Louveau I., Sébert S.B., Damon M., Champ M.M., Gondret F. Cellular and biochemical features of skeletal muscle in obese Yucatan minipigs. Obesity 2006, 14:1700-1707.
17. Kim T.H., Choi B.H., Chang G.W., Lee K.T., Lee H.Y., Lee J.H., et al. Molecular characterization and chromosomal mapping of porcine adipose differentiation-related protein (ADRP). Journal of Animal Breeding and Genetics 2005, 122:240-246.
18. Kimmel A.R., Brasaemle D.L., McAndrews-Hill M., Sztalryd C., Londos C. Adoption of perilipin as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins. Journal of Lipid Research 2010, 51:468-471.
19. Lefaucheur L. Myofiber typing and pig meat production. Slovenian Veterinary Research 2001, 38:5-28.
20. Lefaucheur L. A second look into fibre typing - Relation to meat quality. Meat Science 2010, 84:257-270.
21. Londos C., Brasaemle D.L., Schultz C.J., Segrest J.P., Kimmel A.R. Perilipins, adrp, and other proteins that associate with intracellular neutral lipid droplets in animal cells. Seminars in Cell and Developmental Biology 1999, 10:51-58.
22. Mak K.M., Ren C., Ponomarenko A., Cao Q., Lieber C.S. Adipose differentiation-related protein is a reliable lipid droplet marker in alcoholic fatty liver of rats. Alcoholism: Clinical and Experimental Research 2008, 32:683-689.
23. Malenfant P., Joanisse D.R., Theriault R., Goodpaster B.H., Kelley D.E., Simoneau J.A. Fat content in individual muscle fibers of lean and obese subjects. International Journal of Obesity 2001, 25:1316-1321.
24. Mazzoni M., Clavenzani P., Minieri L., Russo D., Chiocchetti R., Lalatta-Costerbosa G. Extrinsic afferents supplying the ovine duodenum and ileum. Research in Veterinary Science 2010, 88:361-368.
25. McManus J., Mowry R.W. Staining methods, histologic and histochemical 1960, Hoeber Medical Division, New York.
26. Minnaard R., Schrauwen P., Schaart G., Jorgensen J.A., Lenaers E., Mensink M., et al. Adipocyte differentiation-related protein and OXPAT in rat and human skeletal muscle: involvement in lipid accumulation and type 2 diabetes mellitus. Journal of Clinical Endocrinology & Metabolism 2009, 94:4077-4085.
27. Nagayama M., Uchida T., Gohara K. Temporal and spatial variations of lipid droplets during adipocyte division and differentiation. Journal of Lipid Research 2007, 48:9-18.
28. Novikoff A.B., Shin W.Y., Drucker J. Mitochondrial localization of oxidative enzymes: staining results with two tetrazolius salts. Journal of Cell Biology 1961, 9:47-61.
29. Phillips S.A., Choe C.C., Ciaraldi T.P., Greenberg A.S., Kong A.P., Baxi S.C., et al. Adipocyte differentiation-related protein in human skeletal muscle: Relationship to insulin sensitivity. Obesity Research 2005, 13:1321-1329.
30. Qi L., Shen H., Larson I., Schaefer E.J., Greenberg A.S., Tregouet D.A., et al. Gender-specific association of a perilipin gene haplotype with obesity risk in a white population. Obesity Research 2004, 12:1758-1765.
31. Russo V., Nanni Costa L. Suitability of pig meat for salting and the production of quality processed products. Pig News & Information 1995, 16:17N-26N.
32. Shaw C.S., Sherlock M., Stewart P.M., Wagenmakers A.J. Adipophilin distribution and colocalisation with lipid droplets in skeletal muscle. Histochemistry and Cell Biology 2009, 131:575-581.
33. Straub B.K., Stoeffel P., Heid H., Zimbelmann R., Schirmacher P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 2008, 47:1936-1946.
34. Tansey J.T., Sztalryd C., Gruia-Gray J., Roush D.L., Zee J.V., Gavrilova O., et al. Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity. Proceedings of the National Academy of Science of the United States of America 2001, 98:6494-6499.
35. Tao X., Jihong Y., Li G., Bin F., Yi Z., Xiaodong C., et al. Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes. Genetic Selection Evolution 2008, 40:215-226.
36. Toniolo L., Patruno M., Maccatrozzo L., Pellegrino M.A., Canepari M., Rossi R., et al. Fast fibres in a large animal: fibre types, contractile properties and myosin expression in pig skeletal muscles. Journal of Experimental Biology 2004, 207:1875-1886.
37. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002, 3. RESEARCH0034.
38. Wang Z., Jiang T., Li J., Proctor G., McManaman J.L., Lucia S., et al. Regulation of renal lipid metabolism, lipid accumulation, and glomerulosclerosis in FVBdb/db mice with type 2 diabetes. Diabetes 2005, 54:2328-2335.
39. Xu J.X., Albrecht E., Viergutz T., Nürnberg G., Zhao R.Q., Wegner J. Perilipin, C/EBPalpha, and C/EBPbeta mRNA abundance in longissimus muscle and different adipose tissues of Holstein and Charolais cattle. Meat Science 2009, 83:120-126.