Stable expression and functional characterization of a human nicotinic acetylcholine receptor with α6β2 properties: Discovery of selective antagonists

British Journal of Pharmacology

Volumn 163, Issue 2, 2011, Pages 313-329

  Capelli, Anna Maria ^a,e   Castelletti, Laura ^a,e   Chen, Yu Hua ^b   Van Der Keyl, Harjeet ^c   Pucci, Luca ^d   Oliosi, Beatrice ^a,e   Salvagno, Cristian ^a,e   Bertani, Barbara ^a,e   Gotti, Cecilia ^d   Powell, Andrew ^b   Mugnaini, Manolo ^a,e  

^a GSK   (Italy)

^b GLAXOSMITHKLINE   (United Kingdom)

^c GLAXOSMITHKLINE   (United States)

^d UNIVERSITY OF MILAN   (Italy)

^e Aptuit Inc   (United States)

Author keywords

6; 3; chimeric subunit; Nicotine acetylcholine receptor; nicotine dependence; Parkinson's disease; point mutation; recombinant; stable expression

Indexed keywords

ALPHA BUNGAROTOXIN; ALPHA CONOTOXIN MII; ALPHA CONOTOXIN PIA; BUNGAROTOXIN RECEPTOR; DIHYDRO BETA ERYTHROIDINE; EPIBATIDINE; IODINE 125; MECAMYLAMINE; METHYLLYCACONITINE; NICOTINIC RECEPTOR; NICOTINIC RECEPTOR ALPHA1BETA1; NICOTINIC RECEPTOR ALPHA3BETA4; NICOTINIC RECEPTOR ALPHA4BETA2; NICOTINIC RECEPTOR ALPHA6; NICOTINIC RECEPTOR ALPHA6BETA2; NICOTINIC RECEPTOR ALPHA6BETA2 ANTAGONIST; NICOTINIC RECEPTOR BETA2; NICOTINIC RECEPTOR BETA3; NICOTINIC RECEPTOR BLOCKING AGENT; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CELL STRAIN HEK293; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG IDENTIFICATION; DRUG POTENCY; DRUG RECEPTOR BINDING; DRUG RESEARCH; DRUG SCREENING; DRUG STRUCTURE; FLUORESCENCE IMAGING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IC 50; IMMOBILIZED CELL; MALE; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHARMACOPHORE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; STABLE EXPRESSION;

ANIMALS; DRUG EVALUATION, PRECLINICAL; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MALE; MODELS, MOLECULAR; MUTATION; NICOTINIC ANTAGONISTS; PROTEIN SUBUNITS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, NICOTINIC; RECOMBINANT FUSION PROTEINS;

EID: 79954527722     PISSN: 00071188     EISSN: 14765381     Source Type: Journal    
DOI: 10.1111/j.1476-5381.2011.01213.x     Document Type: Article

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