Assessing the components of the eIF3 complex and their phosphorylation status

Journal of Proteome Research

Volumn 10, Issue 4, 2011, Pages 1481-1494

  Farley, Adam R ^a   Powell, David W ^a,b   Weaver, Connie M ^a   Jennings, Jennifer L ^a   Link, Andrew J ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b University of Louisville School of Medicine   (United States)

Author keywords

eIF3; IMAC; mass spectrometry; Nip1; phosphorylation; protein complex; protein kinase; translation; yeast

Indexed keywords

CASEIN KINASE II; INITIATION FACTOR; INITIATION FACTOR 3; INITIATION FACTOR FUN12; INITIATION FACTOR HCR1; INITIATION FACTOR PAB1; INITIATION FACTOR SUI1; INITIATION FACTOR SUI3; INITIATION FACTOR TIF5; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; IN VITRO STUDY; IN VIVO STUDY; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, LIQUID; EUKARYOTIC INITIATION FACTOR-3; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PHOSPHOPEPTIDES; PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TANDEM MASS SPECTROMETRY;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 79953725761     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr100877m     Document Type: Article

References (78)

1. Kimball, S. R. Eukaryotic initiation factor eIF2 Int. J. Biochem. Cell Biol. 1999, 31, 25-29 (Pubitemid 29094786)
2. Unbehaun, A.; Borukhov, S. I.; Hellen, C. U.; Pestova, T. V. Release of initiation factors from 48S complexes during ribosomal subunit joining and the link between establishment of codon-anticodon base-pairing and hydrolysis of eIF2-bound GTP Genes Dev. 2004, 18, 3078-3093 (Pubitemid 39658176)
3. Asano, K.; Clayton, J.; Shalev, A.; Hinnebusch, A. G. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate In Vivo Genes Dev. 2000, 14, 2534-2546
4. Asano, K.; Phan, L.; Valasek, L.; Schoenfeld, L. W.; Shalev, A.; Clayton, J.; Nielsen, K.; Donahue, T. F.; Hinnebusch, A. G. A multifactor complex of eIF1, eIF2, eIF3, eIF5, and tRNA(i)Met promotes initiation complex assembly and couples GTP hydrolysis to AUG recognition Cold Spring Harb. Symp. Quant. Biol. 2001, 66, 403-415 (Pubitemid 36097948)
5. Valasek, L.; Nielsen, K. H.; Hinnebusch, A. G. Direct eIF2-eIF3 contact in the multifactor complex is important for translation initiation In Vivo Embo J. 2002, 21, 5886-5898 (Pubitemid 35315286)
6. Valasek, L.; Mathew, A. A.; Shin, B. S.; Nielsen, K. H.; Szamecz, B.; Hinnebusch, A. G. The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome In Vivo Genes Dev. 2003, 17, 786-799 (Pubitemid 36359196)
7. Olsen, D. S.; Savner, E. M.; Mathew, A.; Zhang, F.; Krishnamoorthy, T.; Phan, L.; Hinnebusch, A. G. Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment In Vivo Embo J. 2003, 22, 193-204 (Pubitemid 36119425)
8. Nielsen, K. H.; Szamecz, B.; Valasek, L.; Jivotovskaya, A.; Shin, B. S.; Hinnebusch, A. G. Functions of eIF3 downstream of 48S assembly impact AUG recognition and GCN4 translational control Embo J. 2004, 23, 1166-1177 (Pubitemid 38436880)
9. Singh, C. R.; Curtis, C.; Yamamoto, Y.; Hall, N. S.; Kruse, D. S.; He, H.; Hannig, E. M.; Asano, K. Eukaryotic translation initiation factor 5 is critical for integrity of the scanning preinitiation complex and accurate control of GCN4 translation Mol. Cell. Biol. 2005, 25, 5480-5491 (Pubitemid 40853583)
10. Hernandez, G.; Vazquez-Pianzola, P. Functional diversity of the eukaryotic translation initiation factors belonging to eIF4 families Mech. Dev. 2005, 122, 865-876 (Pubitemid 40961549)
11. Hinnebusch, A. G.; Asano, K.; Olsen, D. S.; Phan, L.; Nielsen, K. H.; Valasek, L. Study of translational control of eukaryotic gene expression using yeast Ann. N.Y. Acad. Sci. 2004, 1038, 60-74 (Pubitemid 40577444)
12. Maag, D.; Fekete, C. A.; Gryczynski, Z.; Lorsch, J. R. A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon Mol. Cell 2005, 17, 265-275 (Pubitemid 40138620)
13. Das, S.; Maitra, U. Mutational analysis of mammalian translation initiation factor 5 (eIF5): role of interaction between the beta subunit of eIF2 and eIF5 in eIF5 function in vitro and In Vivo Mol. Cell. Biol. 2000, 20, 3942-3950 (Pubitemid 30314493)
14. Das, S.; Maitra, U. Functional significance and mechanism of eIF5-promoted GTP hydrolysis in eukaryotic translation initiation Prog. Nucleic Acid Res. Mol. Biol. 2001, 70, 207-231 (Pubitemid 33804687)
15. Browning, K. S.; Gallie, D. R.; Hershey, J. W.; Hinnebusch, A. G.; Maitra, U.; Merrick, W. C.; Norbury, C. Unified nomenclature for the subunits of eukaryotic initiation factor 3 Trends Biochem. Sci. 2001, 26, 284 (Pubitemid 32436376)
16. Valasek, L.; Phan, L.; Schoenfeld, L. W.; Valaskova, V.; Hinnebusch, A. G. Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding Embo J. 2001, 20, 891-904 (Pubitemid 32167653)
17. Valasek, L.; Nielsen, K. H.; Zhang, F.; Fekete, C. A.; Hinnebusch, A. G. Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection Mol. Cell. Biol. 2004, 24, 9437-9455 (Pubitemid 39391681)
18. Cuchalova, L.; Kouba, T.; Herrmannova, A.; Danyi, I.; Chiu, W. L.; Valasek, L. The RNA recognition motif of eukaryotic translation initiation factor 3g (eIF3g) is required for resumption of scanning of posttermination ribosomes for reinitiation on GCN4 and together with eIF3i stimulates linear scanning Mol. Cell. Biol. 2010, 30, 4671-4686
19. Sonenberg, N.; Hershey, J. W. B.; Mathews, M. B., Eds. Translational Control of Gene Expression; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 2000.
20. Klann, E.; Dever, T. E. Biochemical mechanisms for translational regulation in synaptic plasticity Nat. Rev. Neurosci. 2004, 5, 931-942 (Pubitemid 39593356)
21. Gray, N. S.; Wodicka, L.; Thunnissen, A. M.; Norman, T. C.; Kwon, S.; Espinoza, F. H.; Morgan, D. O.; Barnes, G.; LeClerc, S.; Meijer, L.; Kim, S. H.; Lockhart, D. J.; Schultz, P. G. Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors Science 1998, 281, 533-538 (Pubitemid 28357362)
22. Sudhakar, A.; Ramachandran, A.; Ghosh, S.; Hasnain, S. E.; Kaufman, R. J.; Ramaiah, K. V. Phosphorylation of serine 51 in initiation factor 2 alpha (eIF2 alpha) promotes complex formation between eIF2 alpha(P) and eIF2B and causes inhibition in the guanine nucleotide exchange activity of eIF2B Biochemistry 2000, 39, 12929-12938
23. Waskiewicz, A. J.; Johnson, J. C.; Penn, B.; Mahalingam, M.; Kimball, S. R.; Cooper, J. A. Phosphorylation of the cap-binding protein eukaryotic translation initiation factor 4E by protein kinase Mnk1 In Vivo Mol. Cell. Biol. 1999, 19, 1871-1880 (Pubitemid 29098245)
24. Gingras, A. C.; Raught, B.; Sonenberg, N. eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation Annu. Rev. Biochem. 1999, 68, 913-963 (Pubitemid 29449212)
25. Pyronnet, S.; Imataka, H.; Gingras, A. C.; Fukunaga, R.; Hunter, T.; Sonenberg, N. Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E Embo J. 1999, 18, 270-279 (Pubitemid 29005042)
26. Scheper, G. C.; Morrice, N. A.; Kleijn, M.; Proud, C. G. The mitogen-activated protein kinase signal-integrating kinase Mnk2 is a eukaryotic initiation factor 4E kinase with high levels of basal activity in mammalian cells Mol. Cell. Biol. 2001, 21, 743-754 (Pubitemid 32104723)
27. Aebersold, R.; Mann, M. Mass spectrometry-based proteomics Nature 2003, 422, 198-207 (Pubitemid 36362757)
28. Farley, A. R.; Link, A. J. Identification and quantification of protein posttranslational modifications Methods Enzymol. 2009, 463, 725-763
29. Mann, M.; Ong, S. E.; Gronborg, M.; Steen, H.; Jensen, O. N.; Pandey, A. Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome Trends Biotechnol. 2002, 20, 261-268 (Pubitemid 34451062)
30. Beausoleil, S. A.; Jedrychowski, M.; Schwartz, D.; Elias, J. E.; Villen, J.; Li, J.; Cohn, M. A.; Cantley, L. C.; Gygi, S. P. Large-scale characterization of HeLa cell nuclear phosphoproteins Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 12130-12135 (Pubitemid 39145408)
31. Rush, J.; Moritz, A.; Lee, K. A.; Guo, A.; Goss, V. L.; Spek, E. J.; Zhang, H.; Zha, X. M.; Polakiewicz, R. D.; Comb, M. J. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells Nat. Biotechnol. 2005, 23, 94-101 (Pubitemid 41724631)
32. Andersson, L.; Porath, J. Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography Anal. Biochem. 1986, 154, 250-254 (Pubitemid 16063367)
33. Ficarro, S. B.; McCleland, M. L.; Stukenberg, P. T.; Burke, D. J.; Ross, M. M.; Shabanowitz, J.; Hunt, D. F.; White, F. M. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae Nat. Biotechnol. 2002, 20, 301-305 (Pubitemid 34205425)
34. Ficarro, S. B.; Salomon, A. R.; Brill, L. M.; Mason, D. E.; Stettler-Gill, M.; Brock, A.; Peters, E. C. Automated immobilized metal affinity chromatography/nano-liquid chromatography/electrospray ionization mass spectrometry platform for profiling protein phosphorylation sites Rapid Commun. Mass Spectrom. 2005, 19, 57-71 (Pubitemid 40065069)
35. Moser, K.; White, F. M. Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS J. Proteome Res. 2006, 5, 98-104 (Pubitemid 43100800)
36. Alberti, S.; Gitler, A. D.; Lindquist, S. A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae Yeast 2007, 24, 913-919 (Pubitemid 47611130)
37. Ishii, T. M.; Zerr, P.; Xia, X. M.; Bond, C. T.; Maylie, J.; Adelman, J. P. Site-directed mutagenesis Methods Enzymol. 1998, 293, 53-71 (Pubitemid 29342481)
38. Ghaemmaghami, S.; Huh, W. K.; Bower, K.; Howson, R. W.; Belle, A.; Dephoure, N.; Oshea, E. K.; Weissman, J. S. Global analysis of protein expression in yeast Nature 2003, 425, 737-741 (Pubitemid 37314318)
39. Powell, D. W.; Weaver, C. M.; Jennings, J. L.; McAfee, K. J.; He, Y.; Weil, P. A.; Link, A. J. Cluster analysis of mass spectrometry data reveals a novel component of SAGA Mol. Cell. Biol. 2004, 24, 7249-7259 (Pubitemid 39014450)
40. Link, A. J.; Fleischer, T. C.; Weaver, C. M.; Gerbasi, V. R.; Jennings, J. L. Purifying protein complexes for mass spectrometry: applications to protein translation Methods 2005, 35, 274-290 (Pubitemid 40255593)
41. Powell, D. W.; Rane, M. J.; Joughin, B. A.; Kalmukova, R.; Hong, J. H.; Tidor, B.; Dean, W. L.; Pierce, W. M.; Klein, J. B.; Yaffe, M. B.; McLeish, K. R. Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding Mol. Cell. Biol. 2003, 23, 5376-5387 (Pubitemid 36950892)
42. Link, A. J.; Eng, J.; Schieltz, D. M.; Carmack, E.; Mize, G. J.; Morris, D. R.; Garvik, B. M.; Yates, J. R., 3rd Direct analysis of protein complexes using mass spectrometry Nat. Biotechnol. 1999, 17, 676-682 (Pubitemid 29316146)
43. Sanders, S. L.; Jennings, J.; Canutescu, A.; Link, A. J.; Weil, P. A. Proteomics of the Eukaryotic Transcription Machinery: Identification of Proteins Associated with Components of Yeast TFIID by Multidimensional Mass Spectrometry Mol. Cell. Biol. 2002, 22, 4723-4738 (Pubitemid 34620408)
44. Sadygov, R. G.; Eng, J.; Durr, E.; Saraf, A.; McDonald, H.; MacCoss, M. J.; Yates, J. R., 3rd Code developments to improve the efficiency of automated MS/MS spectra interpretation J. Proteome Res. 2002, 1, 211-215 (Pubitemid 36050250)
45. McAfee, K. J.; Duncan, D. T.; Assink, M.; Link, A. J. Analyzing proteomes and protein function using graphical comparative analysis of tandem mass spectrometry results Mol. Cell. Proteomics 2006, 5, 1497-1513 (Pubitemid 44323302)
46. Yates, J. R., 3rd; Eng, J. K.; McCormack, A. L.; Schieltz, D. Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database Anal. Chem. 1995, 67, 1426-1436
47. Maiti, T.; Bandyopadhyay, A.; Maitra, U. Casein kinase II phosphorylates translation initiation factor 5 (eIF5) in Saccharomyces cerevisiae Yeast 2003, 20, 97-108 (Pubitemid 36131375)
48. Sotanaphun, U.; Phattanawasin, P.; Sriphong, L. Application of Scion image software to the simultaneous determination of curcuminoids in turmeric (Curcuma longa) Phytochem. Anal. 2009, 20, 19-23
49. Zhou, H.; Watts, J. D.; Aebersold, R. A systematic approach to the analysis of protein phosphorylation Nat. Biotechnol. 2001, 19, 375-378 (Pubitemid 32275349)
50. Knippschild, U.; Milne, D. M.; Campbell, L. E.; DeMaggio, A. J.; Christenson, E.; Hoekstra, M. F.; Meek, D. W. p53 is phosphorylated in vitro and In Vivo by the delta and epsilon isoforms of casein kinase 1 and enhances the level of casein kinase 1 delta in response to topoisomerase-directed drugs Oncogene 1997, 15, 1727-1736 (Pubitemid 27466713)
51. Pagano, M. A.; Poletto, G.; Di Maira, G.; Cozza, G.; Ruzzene, M.; Sarno, S.; Bain, J.; Elliott, M.; Moro, S.; Zagotto, G.; Meggio, F.; Pinna, L. A. Tetrabromocinnamic acid (TBCA) and related compounds represent a new class of specific protein kinase CK2 inhibitors Chembiochem 2007, 8, 129-139 (Pubitemid 46121580)
52. Rigaut, G.; Shevchenko, A.; Rutz, B.; Wilm, M.; Mann, M.; Seraphin, B. A generic protein purification method for protein complex characterization and proteome exploration Nat. Biotechnol. 1999, 17, 1030-1032 (Pubitemid 29474865)
53. Gavin, A. C.; Bosche, M.; Krause, R.; Grandi, P.; Marzioch, M.; Bauer, A.; Schultz, J.; Rick, J. M.; Michon, A. M.; Cruciat, C. M.; Remor, M.; Hofert, C.; Schelder, M.; Brajenovic, M.; Ruffner, H.; Merino, A.; Klein, K.; Hudak, M.; Dickson, D.; Rudi, T.; Gnau, V.; Bauch, A.; Bastuck, S.; Huhse, B.; Leutwein, C.; Heurtier, M. A.; Copley, R. R.; Edelmann, A.; Querfurth, E.; Rybin, V.; Drewes, G.; Raida, M.; Bouwmeester, T.; Bork, P.; Seraphin, B.; Kuster, B.; Neubauer, G.; Superti-Furga, G. Functional organization of the yeast proteome by systematic analysis of protein complexes Nature 2002, 415, 141-147 (Pubitemid 34059510)
54. Sanders, S. L.; Garbett, K. A.; Weil, P. A. Molecular characterization of Saccharomyces cerevisiae TFIID Mol. Cell. Biol. 2002, 22, 6000-6013 (Pubitemid 34815846)
55. Fleischer, T. C.; Weaver, C. M.; McAfee, K. J.; Jennings, J. L.; Link, A. J. Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes Genes Dev. 2006, 20, 1294-1307 (Pubitemid 43727590)
56. Ackermann, K.; Waxmann, A.; Glover, C. V.; Pyerin, W. Genes targeted by protein kinase CK2: a genome-wide expression array analysis in yeast Mol. Cell. Biochem. 2001, 227, 59-66 (Pubitemid 34048368)
57. Pinna, L. A. Protein kinase CK2: a challenge to canons J. Cell Sci. 2002, 115, 3873-3878 (Pubitemid 35256488)
58. MacCoss, M. J.; McDonald, W. H.; Saraf, A.; Sadygov, R.; Clark, J. M.; Tasto, J. J.; Gould, K. L.; Wolters, D.; Washburn, M.; Weiss, A.; Clark, J. I.; Yates, J. R., 3rd Shotgun identification of protein modifications from protein complexes and lens tissue Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 7900-7905 (Pubitemid 34650977)
59. Yates, J. R., 3rd; Eng, J. K.; McCormack, A. L. Mining genomes: correlating tandem mass spectra of modified and unmodified peptides to sequences in nucleotide databases Anal. Chem. 1995, 67, 3202-3210
60. Obenauer, J. C.; Cantley, L. C.; Yaffe, M. B. Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifs Nucleic Acids Res. 2003, 31, 3635-3641 (Pubitemid 37442212)
61. Bodenmiller, B.; Mueller, L. N.; Mueller, M.; Domon, B.; Aebersold, R. Reproducible isolation of distinct, overlapping segments of the phosphoproteome Nat. Methods 2007, 4, 231-237 (Pubitemid 46358873)
62. Pestova, T. V.; Lomakin, I. B.; Lee, J. H.; Choi, S. K.; Dever, T. E.; Hellen, C. U. The joining of ribosomal subunits in eukaryotes requires eIF5B Nature 2000, 403, 332-335 (Pubitemid 30062392)
63. Choi, S. K.; Lee, J. H.; Zoll, W. L.; Merrick, W. C.; Dever, T. E. Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast Science 1998, 280, 1757-1760 (Pubitemid 28283500)
64. Kessler, S. H.; Sachs, A. B. RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G Mol. Cell. Biol. 1998, 18, 51-57 (Pubitemid 28021016)
65. Gray, N. K.; Coller, J. M.; Dickson, K. S.; Wickens, M. Multiple portions of poly(A)-binding protein stimulate translation In Vivo Embo J. 2000, 19, 4723-4733
66. Cosson, B.; Berkova, N.; Couturier, A.; Chabelskaya, S.; Philippe, M.; Zhouravleva, G. Poly(A)-binding protein and eRF3 are associated In Vivo in human and Xenopus cells Biol. Cell 2002, 94, 205-216 (Pubitemid 35363818)
67. Tarun, S. Z., Jr.; Sachs, A. B. Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G Embo J. 1996, 15, 7168-7177 (Pubitemid 27023287)
68. Sachs, A.; Davis, R. The poly(A)-binding protein is required for translation initiation and poly(A) tail shortening Mol. Biol. Rep. 1990, 14, 73
69. Gruhler, A.; Olsen, J. V.; Mohammed, S.; Mortensen, P.; Faergeman, N. J.; Mann, M.; Jensen, O. N. Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway Mol. Cell. Proteomics 2005, 4, 310-327 (Pubitemid 40439774)
70. Gerber, S. A.; Rush, J.; Stemman, O.; Kirschner, M. W.; Gygi, S. P. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 6940-6945 (Pubitemid 36706372)
71. Chang, E. J.; Archambault, V.; McLachlin, D. T.; Krutchinsky, A. N.; Chait, B. T. Analysis of protein phosphorylation by hypothesis-driven multiple-stage mass spectrometry Anal. Chem. 2004, 76, 4472-4483 (Pubitemid 39014023)
72. Bodnar, W. M.; Blackburn, R. K.; Krise, J. M.; Moseley, M. A. Exploiting the complementary nature of LC/MALDI/MS/MS and LC/ESI/MS/MS for increased proteome coverage J. Am. Soc. Mass Spectrom. 2003, 14, 971-979 (Pubitemid 37040758)
73. Zhen, Y.; Xu, N.; Richardson, B.; Becklin, R.; Savage, J. R.; Blake, K.; Peltier, J. M. Development of an LC-MALDI method for the analysis of protein complexes J. Am. Soc. Mass Spectrom. 2004, 15, 803-822 (Pubitemid 38656021)
74. Glover, C. V., 3rd On the physiological role of casein kinase II in Saccharomyces cerevisiae Prog. Nucleic Acid Res. Mol. Biol. 1998, 59, 95-133
75. Padmanabha, R.; Chen-Wu, J. L.; Hanna, D. E.; Glover, C. V. Isolation, sequencing, and disruption of the yeast CKA2 gene: casein kinase II is essential for viability in Saccharomyces cerevisiae Mol. Cell. Biol. 1990, 10, 4089-4099
76. Bidwai, A. P.; Reed, J. C.; Glover, C. V. Cloning and disruption of CKB1, the gene encoding the 38-kDa beta subunit of Saccharomyces cerevisiae casein kinase II (CKII). Deletion of CKII regulatory subunits elicits a salt-sensitive phenotype J. Biol. Chem. 1995, 270, 10395-10404
77. Feng, L.; Yoon, H.; Donahue, T. F. Casein kinase II mediates multiple phosphorylation of Saccharomyces cerevisiae eIF-2 alpha (encoded by SUI2), which is required for optimal eIF-2 function in S. cerevisiae Mol. Cell. Biol. 1994, 14, 5139-5153 (Pubitemid 24235942)
78. Zanchin, N. I.; McCarthy, J. E. Characterization of the In Vivo phosphorylation sites of the mRNA.cap-binding complex proteins eukaryotic initiation factor-4E and p20 in Saccharomyces cerevisiae J. Biol. Chem. 1995, 270, 26505-26510