Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding

EMBO Journal

Volumn 20, Issue 4, 2001, Pages 891-904

  Valasek L ^a   Phan, L ^b   Schoenfeld, L W ^b   Valaskova V ^b   Hinnebusch, A G ^b  

^a Laboratory of Gene Regulation and Development   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

40S ribosome; eIF3; HCR1; PRT1 RRM; Translation initiation

Indexed keywords

INITIATION FACTOR 3; MESSENGER RNA; METHIONINE TRANSFER RNA; RIBOSOME RNA; RNA 40S; UNCLASSIFIED DRUG;

ARTICLE; GENE REPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING; SACCHAROMYCES CEREVISIAE; TRANSLATION INITIATION;

ALLELES; AMINO ACID SEQUENCE; CELL CYCLE PROTEINS; FUNGAL PROTEINS; GENES, SUPPRESSOR; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDE INITIATION FACTORS; PROKARYOTIC INITIATION FACTOR-3; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; RIBOSOMES; RNA, FUNGAL; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

SACCHAROMYCES CEREVISIAE;

EID: 0035865256     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.4.891     Document Type: Article

References (34)

1. Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits: Possible roles in RNA binding and macromolecular assembly
2. Complex formation by all five homologues of mammalian translation initiation factor 3 subunits from yeast Saccharomyces cerevisiae
3. Conserved bipartite motifs in yeast eIF5 and eIF2Bε, GTPase-activating and GDP·GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2
4. Met is an important translation initiation intermediate in vivo
5. Cloning and characterization of the p42 subunit of mammalian translation initiation factor 3 (eIF3): Demonstration that eIF3 interacts with eIF5 in mammalian cells
6. Characterization of cDNAs encoding the p44 and p35 subunits of human translation initiation factor elF3
7. Distinct functions of eukaryotic translation initiation factors eIF1A and eIF3 in the formation of the 40S ribosomal preinitiation complex
8. Complex formation by positive and negative translational regulators of GCN4
9. Isolation of a protein complex containing translation initiation factor Prt1 from Saccharomyces cerevisiae
10. Mutational analysis of the Prt1 protein subunit of yeast translation initiation factor 3
11. Structure and interactions of the translation initiation factor eIF1
12. GCD2, a translational repressor of the GCN4 gene, has a general function in the initiation of protein synthesis in Saccharomyces cerevisiae
13. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
14. Characterization of the p33 subunit of eukaryotic translation initiation factor-3 from Saccharomyces cerevisiae
15. GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae
16. RNA recognition motif 2 of yeast Pablp is required for its functional interaction with eukaryotic translation initiation factor 4G
17. Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3′-end formation in yeast
18. Protein trans-acting factors involved in ribosome biogenesis in Saccharomyces cerevisiae
19. Kinetic dissection of fundamental processes of eukaryotic translation initiation in vitro
20. The pathway and mechanism of eukaryotic protein synthesis
21. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170
22. Purified yeast translational initiation factor eIF-3 is an RNA-binding protein complex that contains the PRT1 protein
23. SUI1/p16 is required for the activity of eukaryotic translation initiation factor 3 in Saccharomyces cerevisiae
24. Eukaryotic ribosomes require initiation factors 1 and 1A to locate initiation codons
25. The joining of ribosomal subunits in eukaryotes requires eIF5B
26. Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5
27. Eukaryotic translation initiation: There are (at least) two sides to every story
28. Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B′ and their cognate RNAs
29. The Saccharomyces cerevisiae homologue of mammalian translation initiation factor 6 does not function as a translation initiation factor
30. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
31. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase
32. Rpg1, the Saccharomyces cerevisiae homologue of the largest subunit of mammalian translation initiation factor 3, is required for translational activity
33. The Saccharomyces cerevisiae HCRI gene encoding a homologue of the p35 subunit of human translation eukaryotic initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpglp subunit of yeast eIF3
34. A 110-kilodalton subunit of translation initiation factor eIF3 and an associated 135-kilodalton protein are encoded by the Saccharomyces cerevisiae TIF32 and TIF31 genes