A multi-bioreactor system for optimal production of malaria vaccines with Pichia pastoris

Biotechnology Journal

Volumn 6, Issue 4, 2011, Pages 437-451

  Fricke, Jens ^a   Pohlmann, Kristof ^a   Tatge, Frithjof ^a   Lang, Roman ^a   Faber, Bart ^b   Luttmann, Reiner ^a  

^a HAMBURG UNIVERSITY OF APPLIED SCIENCES   (Germany)

^b BPRC Biomedical Primate Research Center of The Netherlands   (Netherlands)

Author keywords

Multi bioreactor system; Process optimization; Sequential parallel DoE method

Indexed keywords

AERATION STRATEGIES; BIOREACTOR SYSTEM; HOST CELLS; MALARIA VACCINE; MULTISTAGE PRODUCTION; ONLINE MONITORING; OPTIMAL OPERATION; OPTIMAL PRODUCTION; PICHIA PASTORIS; PROCESS ANALYTICAL TECHNOLOGY; PROCESS LAYOUT; PROCESS OPTIMIZATION; PRODUCTION PROCESS; RECOMBINANT PROTEIN SECRETION; SEQUENTIAL/PARALLEL DOE-METHOD; SIMULTANEOUS IDENTIFICATION; SPECIFIC REACTION PARAMETERS; STRAIN CHARACTERIZATION; TARGET PROTEINS;

AUTOMATION; BIOCONVERSION; BIOREACTORS; DESIGN OF EXPERIMENTS; PHYSIOLOGY; PROCESS CONTROL; PRODUCTION ENGINEERING; REACTION KINETICS;

OPTIMIZATION;

MALARIA VACCINE; RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; FUNGAL STRAIN; IMMUNOLOGICAL PARAMETERS; LABORATORY AUTOMATION; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN QUALITY; PROTEIN SECRETION; QUALITY CONTROL; REACTOR MONITORING; VACCINE PRODUCTION;

BIOREACTORS; MALARIA VACCINES; PICHIA;

PICHIA PASTORIS;

EID: 79953715955     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000385     Document Type: Article

References (35)

1. Buckholz, R. G., Gleeson, M. A., Yeast systems for the commercial production of heterologous proteins, Biotechnology (NY) 1991, 9, 1067-1072.
2. Romanos, M., Advances in the use of Pichia pastoris for high-level gene expression, Curr. Opin. Biotechnol. 1995, 6, 527-533.
3. De Hoop, M. J., Cregg, J., Keizer-Gunnink, I., Sjollema, K. et al., Overexpression of alcohol oxidase in Pichia pastoris. FEBS Let. 1991, 291, 299-302.
4. Remarque, E. J., Faber, B. W., Kocken, C. H. M., Thomas, A. W., A diversity-covering approach to immunization with Plasmodium falciparum apical membrane antigen 1 induces broader allelic recognition and growth inhibition responses in rabbit. Infect. Immun. 2008, 76, 2660-2670.
5. Osier, F. H. A., Weedall, G. D., Verra, F., Murungi, L. et al., Allelic diversity and naturally acquired allele-specific antibody responses to Plasmodium falciparum apical membrane antigen 1 in Kenya. Infect. Immun. 2010, 78, 4625-4633.
6. Porro, D., Sauer, M., Branduardi, P., Mattanovich, D., Recombinant protein production in yeasts, Molecular Biotechnology 2005, 31, 245-260.
7. Sreekrishna, K., Brankamp, R. G., Kropp, K. E., Blankenship, D. T. et al., Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris, Gene 1997, 190, 55-62.
8. Fu, J., Wilson, D. B., Shuler, M. L., Continuous, high level production and excretion of a plasmid-encoded protein by Escherichia coli in a two-stage chemostat. Biotechnol. Bioeng. 1993, 41, 937-946.
9. Park, S. H., Ryu, D. D. Y., Kim, J. Y., Effect of cell growth rate on the performance of a two-stage continuous culture system in a recombinant Escherichia coli fermentation. Biotechnol. Bioeng. 1990, 36, 493-505.
10. Curless, C., Fu, K., Swank, R., Menjares, A. et al., Design and evaluation of a two-stage, cyclic, recombinant fermentation process. Biotechnol. Bioeng. 1991, 38, 1082-1090.
11. Lee, S. B., Ryu, D. D. Y., Seigel, S., Park, S. H., Performance of recombinant fermentation and evaluation of gene expression efficiency for gene product in a two-stage continuous culture system. Biotechnol. Bioeng. 1988, 31, 805-820.
12. Zhang, J., Kalogerakis, N., Behie, L. A., Iatrou, K., A two-stage bioreactor system for the production of recombinant proteins using genetically engineered Baculovirus/Insect cell system. Biotechnol. Bioeng. 1993, 42, 357-366.
13. Chang, C. C., Ryu, D. D. Y., Park, C. S., Kim, J.-Y. et al., Recombinant bioprocess optimization for heterologous protein production using two-stage, cyclic fed-batch culture. Appl. Microbiol. Biotechnol. 1998, 49, 531-537.
14. Cornelissen, G., Bertelsen, H. P., Lenz, K., Hahn, B. et al., Production of recombinant proteins with Pichia pastoris in integrated processing. Eng. Life Sci. 2003, 3, 361-370.
15. Hazeu, W., Donker, R. A., A continuous culture study of methanol and format utilization by the yeast Pichia pastoris. Biotechnol. Lett. 1983, 5, 399-404.
16. Briggs, G. E., Haldane, J. B. S., A note on the kinetics of enzyme reactions. Biochem. J. 1925, 19, 338-33.
17. Lokshina, L., Vavlin, V., Kettunen, R. H., Rintala, J. A. et al., Evaluation of kinetic coefficients using integrated monod and haldane models for low-temperature acetoclastic methanogenesis. Water Res. 2001, 35, 2913-2922.
18. Zhang, W., Bevins, M. A., Plantz, B. A., Smith, L. A. et al., Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, Serotype A. Biotechnol. Bioeng. 2000, 70, 1-8.
19. th Intern. Symp. on Computer Appl. Biotechnol. 2010, 383-388. DOI: 10.3182/20100707-3-BE-2012.020
20. Curvers, S., Linnemann, J., Klauser, T., Wandrey, C. et al., Recombinant protein production with Pichia pastoris in continuous fermentation - kinetic analysis of growth and production formation, Chemie Ingenieur Technik 2001, 73, 1615-1621.
21. s fermentation, Periodica Polytechnica Ser. Chem. Eng. 2004, 48, 73-87.
22. Pais, J. M., Varas, L., Valdes, J., Cabello, C. et al., Modeling of mini-proinsulin production in Pichia pastoris using the AOX promoter, Biotechnol. Lett. 2003, 25, 251-25.
23. Cos, O., Ramon, R., Montesinos, J. L., Valero, F., A simple model-based control for Pichia pastoris allows a more efficient heterologous protein production bioprocess, Biotechnol. Bioeng. 2006, 95. 145-164.
24. Stratton, J., Chiruvula, V., Meagher, M., High cell-density fermentation, in: Higgins, D. R., Cregg, J. M., Pichia protocols - Methods in Molecular Biology, Humana Press, Totowa 1998, 107-120.
25. Files, D., Ogawa, M., Scaman, C. H., Baldwin, S. A., A Pichia pastoris fermentation process for producing high-levels of recombinant human cystacin-C. Enzyme Microbial Technol. 2001, 29, 335-340.
26. Andre, N., Cherouati, N., Prual, C., Steffan, T. et al., Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci. 2006, 15, 1115-1126.
27. Jahic, M., Gustavsson, M., Jansen, A. K., Martinelle, M. et al., Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J. Biotechnol 2003, 102, 45-53.
28. Jahic, M., Wallberg, F., Bollok, M., Garcia, P. et al., Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb. Cell Fact. 2003, 2, DOI: 10.1186/1475-2859-2-6.
29. Cregg, J. M., Vedvick, T. S., Raschke, W. C., Recent advances in the expression of foreign genes in Pichia pastoris. Bio/Technology 1993, 11, 905-910.
30. Eriksson, L., Johansson, E., Kettaneh-Wold, N., Wikström, C., et al., Design of experiments - principles and applications, MKS Umetrics AB, Umea, 2008.
31. Inan, M., Chiruvolu, V., Eskridge, K. M., Vlasuk, P. et al., Optimization of temperature-glycerol-pH conditions for a fed-batch fermentation process for recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (AcAP-5) production by Pichia pastoris, Enz. Microbiol. Technol. 1999, 24, 438-445.
32. Macauley-Patrick, S., Fazenda, M. L., McNeil, B., Harvey, L. M., Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22, 249-270.
33. Steinle, A., Witthoff, S., Krause, J. P., Steinbüchel, A., Establishment of cyanophycin biosynthesis in Pichia pastoris and optimization by use of engineered cyanophycin synthetases, Appl. Environ. Microbiol. 2010, 76, 1062-1070.
34. Sinha, J., Plantz, B. A., Zhang, W., Gouthro, M. et al., Improved production of recombinant ovine interferon-t by Mut+ strain of Pichia pastoris using optimized methanol feed profile, Biotechnol. Prog. 2003, 19, 794-802.
35. Loegering, K., Mueller, C., Voss, J. P., Wagenführer, C. et al., Development of an optimal production process of recombinant enzymes with Pichia pastoris in an integrated scale down production plant, Biotechnology J. 2011, 6, DOI: 10.1002/biot.201000384.