Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability

Protein Science

Volumn 17, Issue 7, 2008, Pages 1275-1284

  Thomaeus, Ann ^a   Naworyta, Agata ^b   Mowbray, Sherry L ^b   Widersten, Mikael ^a,c  

^a UPPSALA UNIVERSITY   (Sweden)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Epoxide hydrolase; Mutants; Proton wire; Thermostability; X ray crystal structure

Indexed keywords

EPOXIDE HYDROLASE; HISTIDINE; HYDROXYL GROUP; IMIDAZOLE; LEUCINE; PROLINE; PROTEIN; PROTON; TRANS STILBENE OXIDE; TYROSINE; WATER;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENANTIOMER; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME STRUCTURE; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY;

CATALYSIS; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; EPOXIDE HYDROLASES; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; PLANTS; PROTEINS; WATER;

SOLANUM TUBEROSUM;

EID: 46449120046     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.034173.107     Document Type: Article

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