Silica-immobilized His6-tagged enzyme: Alanine racemase in hydrophobic solvent

Biotechnology and Bioengineering

Volumn 99, Issue 3, 2008, Pages 712-716

  Cassimjee, Karim Engelmark ^a   Trummer, Martin ^a   Branneby, Cecilia ^a   Berglund, Per ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Enzyme immobilization; Enzyme surface; Geobacillus stearothermophilus; His6 tag

Indexed keywords

BACTERIA; ENZYME IMMOBILIZATION; HYDROPHOBICITY; SILICA; THIN LAYER CHROMATOGRAPHY;

ENZYME SURFACE; GEOBACILLUS STEAROTHERMOPHILUS;

ENZYME ACTIVITY;

ALANINE RACEMASE; COBALT; HEXANE; HISTONE; ORGANIC SOLVENT; SILICON DIOXIDE; HISTIDINE; IMMOBILIZED ENZYME; SOLVENT;

AQUEOUS SOLUTION; ARTICLE; ENZYME IMMOBILIZATION; ENZYME SYNTHESIS; FREEZING; GEOBACILLUS STEAROTHERMOPHILUS; HYDROPHOBICITY; IMMERSION; NONHUMAN; PROTEIN EXPRESSION; THIN LAYER CHROMATOGRAPHY; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; GEOBACTER;

GEOBACILLUS STEAROTHERMOPHILUS;

ALANINE RACEMASE; ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; GEOBACTER; HISTIDINE; HYDROPHOBICITY; SILICON DIOXIDE; SOLVENTS;

EID: 38449117584     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21587     Document Type: Article

References (20)

1. Bornscheuer UT. 2003. Immobilizing enzymes: How to create more suitable biocatalysts. Angew Chem Int Ed 42:3336-3337.
2. Cao L. 2005. Immobilised enzymes: Science or art? Curr Opin Chem Biol 9:217-226.
3. Cao L, van Langen L, Sheldon RA. 2003. Immobilised enzymes: Carrier-bound or carrier-free? Curr Opin Biotechnol 14:387-394.
4. Chen ST, Huang WH, Wang KT. 1994. Resolution of amino acids in a mixture of 2-methyl-2-propanol/water (19:1) catalyzed by alcalase via in situ racemization of one antipode mediated by pyridoxal 5-phosphate. J Org Chem 59:7580-7581.
5. Copie V, Faraci WS, Walsh CT, Griffin RG. 1988. Inhibition of alanine racemase by alanine phosphonate: Detection of an imine linkage to pyridoxal 5′-phosphate in the enzyme-inhibitor complex by solid-state 15N nuclear magnetic resonance. Biochemistry 27:4966-4970.
6. Goswami A, Singh AK. Silica gel functionalized with resacetophenone: Synthesis of a new chelating matrix and its application as metal ion collector for their flame atomic absorption spectrometric determination. Anal Chim Acta 454:229-240.
7. Hacking MAPJ, Wegman MA, Rops J, van Rantwijk F, Sheldon RA. 1998. Enantioselective synthesis of amino acid amides via enzymatic ammoniolysis of amino acid esters. J Mol Catal B: Enzym 5:155-157.
8. Inagaki K, Tanizawa K, Badet B, Walsh CT, Tanaka H, Soda K. 1986. Thermostable alanine racemase from Bacillus stearothermophilus: Molecular cloning of the gene, enzyme purification, and characterization. Biochemistry 25:3268-3274.
9. Lauer SA, Nolan JP. 2002. Development and characterization of Ni-NTA-bearing microspheres. Cytometry 48:136-145.
10. Luckarift HR, Spain JC, Naik RR, Stone MO. 2004. Enzyme immobilization in a biomimetic silica support. Nat Biotechnol 22:211-213.
11. Luckarift HR, Johnson GR, Spain JC. 2006. Silica-immobilized enzyme reactors; application to cholinesterase-inhibition studies. J Chromatogr B: Anal Technol Biomed Life Sci 843:310-316.
12. Major DT, Nam K, Gao J. 2006. Transition state stabilization and alpha-amino carbon acidity in alanine racemase. J Am Chem Soc 128:8114-8115.
13. Miller SA, Hong ED, Wright D. 2006. Rapid and efficient enzyme encapsulation in a dendrimer silica nanocomposite. Macromol Biosci 6:839-845.
14. Miyazaki M, Kaneno J, Yamaori S, Honda T, Briones MP, Uehara M, Arima K, Kanno K, Yamashita K, Yamaguchi Y, Nakamura H, Yonezawa H, Fujii M, Maeda H. 2005. Efficient immobilization of enzymes on microchannel surface through His-tag and application for microreactor. Protein Pept Lett 12:207-210.
15. Shaw JP, Petsko GA, Ringe D. 1997. Determination of the structure of alanine racemase from Bacillus stearothermophilus at 1.9-A resolution. Biochemistry 36:1329-1342.
16. Steinreiber J, Schürmann M, Wolberg M, van Assema F, Reisinger C, Fesko K, Mink D, Griengl H. 2007. Overcoming thermodynamic and kinetic limitations of aldolase-catalyzed reactions by applying multienzymatic dynamic kinetic asymmetric transformations. Angew Chem Int Ed 46:1624-1626.
17. Tachibana S, Suzuki M, Asano Y. 2006. Application of an enzyme chip to the microquantification of 1-phenylalanine. Anal Biochem 359:72-78.
18. Tanizawa K, Ohshima A, Scheidegger A, Inagaki K, Tanaka H, Soda K. 1988. Thermostable alanine racemase from Bacillus stearothermophilus: DNA and protein sequence determination and secondary structure prediction. Biochemistry 27:1311-1316.
19. Watanabe A, Yoshimura T, Mikami B, Hayashi H, Kagamiyama H, Esaki N. 2002. Reaction mechanism of alanine racemase from Bacillus stearothermophilus: X-ray crystallographic studies of the enzyme bound with N-(5′-phosphopyridoxyl)alanine. J Biol Chem 277:19166-19172.
20. Wegman MA, Hacking MAPJ, Rops J, Pereira P, van Rantwijk F, Sheldon RA. 1999. Dynamic kinetic resolution of phenylglycine esters via lipase-catalysed ammonolysis. Tetrahedron: Asymmetry 10:1739-1750.