Expression of water-soluble, ligand-binding concatameric extracellular domains of the human neuronal nicotinic receptor α4 and β2 subunits in the yeast Pichia pastoris:Glycosylation is not required for ligand binding

Journal of Biological Chemistry

Volumn 286, Issue 11, 2011, Pages 8884-8892

  Stergiou, Christos ^a,b   Zisimopoulou, Paraskevi ^a   Tzartos, Socrates J ^a,b  

^a HELLENIC PASTEUR INSTITUTE   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE-BINDING PROTEINS; ALZHEIMER; AMINO ACID PEPTIDES; APPROPRIATE MATERIALS; CATION CHANNELS; CELL COMMUNICATION; COEXPRESSED; DEGLYCOSYLATION; DISSOCIATION CONSTANT; DRUG DESIGN; EPIBATIDINE; EUKARYOTIC EXPRESSION; EXTRACELLULAR DOMAINS; HIGH AFFINITY; INHIBITION CONSTANTS; LIGAND BINDING; LIGAND BINDING DOMAIN; LIGAND BINDING PROPERTIES; MAMMALIAN BRAIN; MONOMERIC FORMS; NICOTINIC ACETYLCHOLINE RECEPTORS (NACHRS); NICOTINIC RECEPTOR; PARKINSON DISEASE; PICHIA PASTORIS; SPECIFIC INTERACTION; STRUCTURAL STUDIES; WATER SOLUBILITIES; WILD TYPES;

AMINO ACIDS; BINDING ENERGY; DISSOCIATION; ELECTROCHROMIC DEVICES; ENZYME ACTIVITY; MAMMALS; PARKS; PYRIDINE; REDUCTION;

LIGANDS;

ACETYLCHOLINE; CARBACHOL; CYTISINE; NICOTINE; NICOTINIC RECEPTOR ALPHA4; NICOTINIC RECEPTOR ALPHA4BETA2; NICOTINIC RECEPTOR BETA2;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CRYSTALLIZATION; DISSOCIATION CONSTANT; GLYCOSYLATION; HYDROPHILICITY; LIGAND BINDING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SOLUBILITY;

GENE EXPRESSION; HUMANS; LIGANDS; MUTATION; NICOTINIC AGONISTS; PICHIA; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, NICOTINIC; RECOMBINANT PROTEINS; SOLUBILITY;

EUKARYOTA; GASTROPODA; MAMMALIA; PICHIA PASTORIS;

EID: 79953223642     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.171645     Document Type: Article

References (45)

1. Corringer, P. J., Le Novère, N., and Changeux, J. P. (2000) Annu. Rev. Pharmacol. Toxicol. 40, 431-458
2. Dennis, M., Giraudat, J., Kotzyba-Hibert, F., Goeldner, M., Hirth, C., Chang, J. Y., Lazure, C., Chrétien, M., and Changeux, J. P. (1988) Biochemistry 27, 2346-2357
3. Galzi, J. L., and Changeux, J. P. (1995) Neuropharmacology 34, 563-582
4. Huganir, R. L., and Greengard, P. (1990) Neuron 5, 555-567
5. Karlin, A. (2002) Nat. Rev. Neurosci. 3, 102-114
6. Kullmann, D. M. (2002) Brain 125, 1177-1195
7. Gotti, C., Zoli, M., and Clementi, F. (2006) Trends Pharmacol. Sci. 27, 482-491
8. Moroni, M., Zwart, R., Sher, E., Cassels, B. K., and Bermudez, I. (2006) Mol. Pharmacol. 70, 755-768
9. Paterson, D., and Nordberg, A. (2000) Prog. Neurobiol. 61, 75-111
10. Perry, E., Martin-Ruiz, C., Lee, M., Griffiths, M., Johnson, M., Piggott, M., Haroutunian, V., Buxbaum, J. D., Nãsland, J., Davis, K., Gotti, C., Clementi, F., Tzartos, S., Cohen, O., Soreq, H., Jaros, E., Perry, R., Ballard, C., McKeith, I., and Court, J. (2000) Eur. J. Pharmacol. 393, 215-222
11. Quik, M., O'Neill, M., and Perez, X. A. (2007) Trends Pharmacol. Sci. 28, 229-235
12. Breese, C. R., Lee, M. J., Adams, C. E., Sullivan, B., Logel, J., Gillen, K. M., Marks, M. J., Collins, A. C., and Leonard, S. (2000) Neuropsychopharmacology 23, 351-364
13. Galanti, L. M. (2008) Vasc. Health Risk Manag. 4, 837-845
14. Arneric, S. P., Holladay, M., and Williams, M. (2007) Biochem. Pharmacol. 74, 1092-1101
15. Brejc, K., van Dijk, W. J., Klaassen, R. V., Schuurmans, M., van Der Oost, J., Smit, A. B., and Sixma, T. K. (2001) Nature 411, 269-276
16. Unwin, N. (1998) J. Struct. Biol. 121, 181-190
17. Dellisanti, C. D., Yao, Y., Stroud, J. C., Wang, Z. Z., and Chen, L. (2007) Nat. Neurosci. 10, 953-962
18. Bocquet, N., Nury, H., Baaden, M., Le Poupon, C., Changeux, J. P., Delarue, M., and Corringer, P. J. (2009) Nature 457, 111-114
19. Hilf, R. J., and Dutzler, R. (2008) Nature 452, 375-379
20. Bisson, W. H., Westera, G., Schubiger, P. A., and Scapozza, L. (2008) J. Mol. Model 14, 891-899
21. Konstantakaki, M., Tzartos, S. J., Poulas, K., and Eliopoulos, E. (2008) J. Mol. Graph. Model 26, 1333-1337
22. Pedretti, A., Marconi, C., Bolchi, C., Fumagalli, L., Ferrara, R., Pallavicini, M., Valoti, E., and Vistoli, G. (2008) Biochem. Biophys. Res. Commun. 369, 648-653
23. Person, A. M., Bills, K. L., Liu, H., Botting, S. K., Lindstrom, J., and Wells, G. B. (2005) J. Biol. Chem. 280, 39990-40002
24. Carbone, A. L., Moroni, M., Groot-Kormelink, P. J., and Bermudez, I. (2009) Br. J. Pharmacol. 156, 970-981
25. Zhou, Y., Nelson, M. E., Kuryatov, A., Choi, C., Cooper, J., and Lindstrom, J. (2003) J. Neurosci. 23, 9004-9015
26. Groot-Kormelink, P. J., Broadbent, S., Beato, M., and Sivilotti, L. G. (2006) Mol. Pharmacol. 69, 558-563
27. Kuryatov, A., and Lindstrom, J. M. (2011) Mol. Pharmacol. 79, 126-140
28. Avramopoulou, V., Mamalaki, A., and Tzartos, S. J. (2004) J. Biol. Chem. 279, 38287-38293
29. Psaridi-Linardaki, L., Mamalaki, A., Remoundos, M., and Tzartos, S. J. (2002) J. Biol. Chem. 277, 26980-26986
30. Zouridakis, M., Zisimopoulou, P., Eliopoulos, E., Poulas, K., and Tzartos, S. J. (2009) Biochim. Biophys. Acta 1794, 355-366
31. Bruns, R. F., Lawson-Wendling, K., and Pugsley, T. A. (1983) Anal. Biochem. 132, 74-81
32. Cheng, Y., and Prusoff, W. H. (1973) Biochem. Pharmacol. 22, 3099-3108
33. Bitzopoulou, K., Kostelidou, K., Poulas, K., and Tzartos, S. J. (2008) Biochim. Biophys. Acta 1784, 1226-1233
34. Wang, D. X., and Abood, L. G. (1996) J. Neurosci. Res. 44, 350-354
35. Perry, D. C., Xiao, Y., Nguyen, H. N., Musachio, J. L., Dávila-García, M. I., and Kellar, K. J. (2002) J. Neurochem. 82, 468-481
36. Whiteaker, P., Jimenez, M., McIntosh, J. M., Collins, A. C., and Marks, M. J. (2000) Br. J. Pharmacol. 131, 729-739
37. Breese, C. R., Marks, M. J., Logel, J., Adams, C. E., Sullivan, B., Collins, A. C., and Leonard, S. (1997) J. Pharmacol. Exp. Ther. 282, 7-13
38. Bencherif, M., Lovette, M. E., Fowler, K. W., Arrington, S., Reeves, L., Caldwell, W. S., and Lippiello, P. M. (1996) J. Pharmacol. Exp. Ther. 279, 1413-1421
39. Chen, Y., Sharples, T. J., Phillips, K. G., Benedetti, G., Broad, L. M., Zwart, R., and Sher, E. (2003) Neuropharmacology 45, 334-344
40. Marks, M. J., Robinson, S. F., and Collins, A. C. (1996) J. Pharmacol. Exp. Ther. 277, 1383-1396
41. Nelson, M. E., Kuryatov, A., Choi, C. H., Zhou, Y., and Lindstrom, J. (2003) Mol. Pharmacol. 63, 332-341
42. Schrattenholz, A., Pfeiffer, S., Pejovic, V., Rudolph, R., Godovac-Zimmermann, J., and Maelicke, A. (1998) J. Biol. Chem. 273, 32393-32399
43. Maley, F., Trimble, R. B., Tarentino, A. L., and Plummer, T. H., Jr. (1989) Anal. Biochem. 180, 195-204
44. Xiao, Y., and Kellar, K. J. (2004) J. Pharmacol. Exp. Ther. 310, 98-107
45. Sharples, C. G., Karig, G., Simpson, G. L., Spencer, J. A., Wright, E., Millar, N. S., Wonnacott, S., and Gallagher, T. (2002) J. Med. Chem. 45, 3235-3245