The cysteine-rich domain of human T1R3 is necessary for the interaction between human T1R2-T1R3 sweet receptors and a sweet-tasting protein, thaumatin

Biochemical and Biophysical Research Communications

Volumn 406, Issue 3, 2011, Pages 435-438

  Ohta, Keisuke ^a   Masuda, Tetsuya ^a   Tani, Fumito ^a   Kitabatake, Naofumi ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Cell based assay; Chimeric human mouse sweet receptors; Sweet receptor; Sweet tasting protein; Thaumatin

Indexed keywords

CYSTEINE; RECEPTOR; SUCRALOSE; T1R2 PROTEIN; T1R3 PROTEIN; THAUMATIN; UNCLASSIFIED DRUG;

ARTICLE; HETEROLOGOUS EXPRESSION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; CYSTEINE; HEK293 CELLS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; TASTE;

RODENTIA;

EID: 79952739560     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.02.063     Document Type: Article

References (21)

1. van der Wel H., Loeve K. Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii Benth. Eur. J. Biochem. 1972, 31:221-225.
2. Iyengar R.B., Smith P., van der Ouderaa F., van der Wel H., van Brouwershaven J., Ravestein P., Richters G., van Wassenaar P.D. The complete amino-acid sequence of the sweet protein thaumatin I. Eur. J. Biochem. 1979, 96:193-204.
3. van der Wel H., Iyengar R.B., van Brouwershaven J., van Wassenaar P.D., Bel W.J., van der Ouderaa F.J. Assignment of the disulphide bonds in the sweet-tasting protein thaumatin I. Eur. J. Biochem. 1984, 144:41-45.
4. Hoon M.A., Adler E., Lindemeier L., Battey J.F., Ryba N.J., Zuker C.S. Putative mammalian taste receptors: a class of taste-specific GPCRs with distinct topographic selectivity. Cell 1999, 96:541-551.
5. Nelson G., Hoon M.A., Chandrashekar J., Zhang Y., Ryba N.J., Zuker C.S. Mammalian sweet taste receptors. Cell 2001, 106:381-390.
6. Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E. Human receptors for sweet and umami taste. Proc. Natl. Acad. Sci. USA 2002, 99:4692-4696.
7. Danilova V., Hellekant G., Roberts T., Tinti J.M., Nofre C. Behavioral and single chorda tympani taste fiber responses in the common marmoset Callithrix jacchus. Ann. N.Y. Acad. Sci. 1998, 855:160-164.
8. Jiang P., Ji Q., Liu Z., Snyder L.A., Benard L.M.J., Margolskee R.F., Max M. The cysteine-rich region of T1R3 determines responses to intensely sweet proteins. J. Biol. Chem. 2004, 279:45068-45075.
9. Koizumi A., Nakajima K., Asakura T., Morita Y., Ito K., Shimizu-Ibuka A., Misaka T., Abe K. Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain. Biochem. Biophys. Res. Commun. 2007, 358:585-589.
10. Assadi-Porter F.M., Maillet E.L., Radek J.T., Quijada J., Markley J.L., Max M. Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor. J. Mol. Biol. 2010, 398:584-599.
11. Temussi P.A. Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2-T1R3 receptor. FEBS Lett. 2002, 526:1-3.
12. Morini G., Bassoli A., Temussi P.A. From small sweeteners to sweet proteins: anatomy of the binding sites of the human T1R2-T1R3 receptor. J. Med. Chem. 2005, 48:5520-5529.
13. Walters D.E., Hellekant G. Interactions of the sweet protein brazzein with the sweet taste receptor. J. Agric. Food Chem. 2006, 54:10129-10133.
14. Ide N., Masuda T., Kitabatake N. Effects of pre- and pro-sequence of thaumatin on the secretion by Pichia pastoris. Biochem. Biophys. Res. Commun. 2007, 363:708-714.
15. Ide N., Sato E., Ohta K., Masuda T., Kitabatake N. Interactions of the sweet-tasting proteins thaumatin and lysozyme with the human sweet-taste receptor. J. Agric. Food Chem. 2009, 57:5884-5890.
16. Ueda T., Ugawa S., Yamamura H., Imaizumi Y., Shimada S. Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cells. J. Neurosci. 2003, 23:7376-7380.
17. Ming D., Hellekant G. Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett. 1994, 355:106-108.
18. Yamashita H., Theeraship A., Nakaya T., Nakamura Y., Kurihara Y. Purification and complete amino acid sequence of a new type of sweet protein with taste-modifying activity, curculin. J. Biol. Chem. 1990, 265:15770-15775.
19. Shirasuka Y., Nakajima K., Asakura T., Yamashita Y., Yamamoto A., Hata S., Nagata S., Abo M., Sorimachi H., Abe K. Neoculin as a new taste-modifying protein occurring in the fruit of Curculigo latifolia. Biosci. Biotechnol. Biochem. 2004, 68:1403-1407.
20. Ohta K., Masuda T., Ide N., Kitabatake N. Critical molecular regions for elicitation of the sweetness of the sweet-tasting protein thaumatin I. FEBS J. 2008, 275:3644-3652.
21. Cui M., Jiang P., Maillet E., Max M., Margolskee R.F., Osman R. The heterodimeric sweet taste receptor has multiple potential ligand binding sites. Curr. Pharm. Des. 2006, 12:4591-4600.