Critical molecular regions for elicitation of the sweetness of the sweet-tasting protein, thaumatin I

FEBS Journal

Volumn 275, Issue 14, 2008, Pages 3644-3652

  Ohta, Keisuke ^a   Masuda, Tetsuya ^a   Ide, Nobuyuki ^a   Kitabatake, Naofumi ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Sweet tasting protein; Sweetness; Thaumatin

Indexed keywords

ALANINE; ARGININE; LYSINE; THAUMATIN; THAUMATIN I; UNCLASSIFIED DRUG; AMINO ACID; SWEETENING AGENT; THAUMATIN PROTEIN, PLANT; VEGETABLE PROTEIN;

ADULT; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN EXPERIMENT; NORMAL HUMAN; PERCEPTIVE THRESHOLD; PRIORITY JOURNAL; PROTEIN CONFORMATION; STRUCTURE ACTIVITY RELATION; SWEETNESS; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; MUTATION; PICHIA; TASTE;

PICHIA PASTORIS;

ALANINE; AMINO ACID SUBSTITUTION; AMINO ACIDS, BASIC; ARGININE; LYSINE; MODELS, MOLECULAR; MUTATION; PICHIA; PLANT PROTEINS; PROTEIN CONFORMATION; SWEETENING AGENTS; TASTE;

EID: 50549103804     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2008.06509.x     Document Type: Article

References (34)

1. van der Wel H & Loeve K (1972) Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii Benth. Eur J Biochem 31, 221-225.
2. Edens L, Heslinga L, Klok R, Ledeboer AM, Maat J, Toonen MY, Visser CT & Verrips T (1982) Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli. Gene 18, 1-12.
3. Ide N, Kaneko R, Wada R, Mehta A, Tamaki S, Tsuruta T, Fujita Y, Masuda T & Kitabatake N (2007a) Cloning of the thaumatin I cDNA and characterization of recombinant thaumatin I secreted by Pichia pastoris. Biotechnol Prog 23, 1023-1030.
4. Iyengar RB, Smith P, van der Ouderaa F, van der Wel H, van Brouwershaven J, Ravestein P, Richters G & van Wassenaar PD (1979) The complete amino-acid sequence of the sweet protein thaumatin I. Eur J Bio-chem 96, 193-204.
5. van der Wel H, Iyenger RB, van Brouwershaven J, van Wassenaar PD, Bel WJ & van der Ouderaa FJ (1984) Assignment of the disulphide bonds in the sweet-tasting protein thaumatin I. Eur J Biochem 144, 41-45.
6. Kaneko R & Kitabatake N (2001) Structure-sweetness relationship study of sweet protein thaumatin: importance of lysine residues. Chem Senses 26, 167-177.
7. Masuda T, Ueno Y & Kitabatake N (2001) Sweetness and enzymatic activity of lysozyme. J Agric Food Chem 49, 4937-4941.
8. Masuda T, Ide N & Kitabatake N (2005) Effects of chemical modification of lysine residues on the sweetness of lysozyme. Chem Senses 30, 253-264.
9. Masuda T, Ide N & Kitabatake N (2005) Structure- sweetness relationship in egg white lysozyme: role of lysine and arginine residues on the elicitation of lyso-zyme sweetness. Chem Senses 30, 667-681.
10. Cereghino JL & Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 1, 45-66.
11. Cregg JM, Cereghino JL, Shi J & Higgins DR (2000) Recombinant protein expression in Pichia pastoris. Mol Biotechnol 16, 23-52.
12. Masuda T, Tamaki S, Kaneko R, Wada R, Fujita Y, Mehta A & Kitabatake N (2004) Cloning, expression and characterization of recombinant sweet-protein thaumatin II using the methylotrophic yeast Pichia pastoris. Biotechnol Bioeng 85, 761-769.
13. Ide N, Masuda T & Kitabatake N (2007) Effects of pre-and pro-sequence of thaumatin on the secretion by Pichia pastoris. Biochem Biophys Res Commun 363, 708-714.
14. van der Wel H (1994) Structure-activity relationships in the thaumatin molecule. In Thaumatin (Witty M & Higginbotham JD, eds), pp. 115-122. CRC Press, Boca Raton, FL.
15. Masuda T & Kitabatake N (2006) Developments in biotechnological production of sweet proteins. J Biosci Bioeng 102, 375-389.
16. Weickmann JL, Blair LC & Wilcox GL (1994) High level expression of thaumatin in Saccharomyces cerevisi-ae. In Thaumatin (Witty M & Higginbotham JD, eds), pp. 151-169. CRC Press, Boca Raton, FL.
17. Raemaekers RJ, de Muro L, Gatehouse JA & Ford-ham-Skelton AP (1999) Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide. Eur J Biochem 265, 394-403.
18. Hoon MA, Adler E, Lindemeier L, Battey JF, Ryba NJP & Zuker CS (1999) Putative mammalian taste receptors: a class of taste-specific GPCRs with distinct topographic selectivity. Cell 96, 541-551.
19. Li X, Staszewski L, Xu H, Durick K, Zoller M & Adler E (2002) Human receptors for sweet and umami taste. Proc Natl Acad Sci USA 99, 4692-4696.
20. Jiang P, Ji Q, Liu Z, Snyder LA, Benard LMJ, Margolskee RF & Max M (2004) The cysteine-rich region of T1R3 determines responses to intensely sweet proteins. J Biol Chem 279, 45068-45075.
21. Morini G, Bassoli A & Temussi PA (2005) From small sweeteners to sweet proteins: anatomy of the binding sites of the human T1R2-T1R3 receptor. J Med Chem 48, 5520-5529.
22. van der Wel H (1972) Isolation and characterization of the sweet principal for Dioscoreophyllum cumminsii (Stapf). FEBS Lett 21, 88-90.
23. Moris JA & Cagan RH (1972) Purification of monellin, the sweet principal for Dioscoreophyllum cumminsii. Bio-chem Biophys Acta 261, 114-122.
24. Liu X, Hu Z, Maeda S, Aiuchi T, Nakaya K & Kuriha-ra Y (1993) Purification, complete amino acid sequence and structure characterization of the heat stable sweet protein, mabinlin II. Eur J Biochem 211, 281-287.
25. Yamashita H, Theeraship A, Nakaya T, Nakamura Y & Kurihara Y (1990) Purification and complete amino acid sequence of a new type of sweet protein with taste-modifying activity, curculin. J Biol Chem 265, 15770- 15775.
26. Shirasuka Y, Nakajima K, Asakura T, Yamashita Y, Yamamoto A, Hata S, Nagata S, Abo M, Sorimachi H & Abe K (2004) Neoculin as a new taste-modifying protein occurring in the fruit of Curculigo latifolia. Biosci Biotechnol Biochem 68, 1403-1407.
27. Ming D & Hellekant G (1994) Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett 355, 106-108.
28. Temussi PA (2002) Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2-T1R3 receptor. FEBS Lett 526, 1-3.
29. Spadaccini R, Trabucco F, Saviano G, Picone D, Crescenzi O, Tancredi T & Temussi PA (2003) The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI. J Mol Biol 328, 683-692.
30. Esposito V, Gallucci R, Picone D, Saviano G, Tancredi T & Temussi PA (2006) The importance of electrostatic potential in the interaction of sweet proteins with the sweet taste receptor. J Mol Biol 360, 448-456.
31. Jin Z, Danilova V, Assadi-Porter FM, Aceti DJ, Mark-ley JL & Hellekant G (2003) Critical regions for the sweetness of brazzein. FEBS Lett 544, 33-37.
32. Kaneko R & Kitabatake N (1999) Heat-induced formation of intermolecular disulfide linkages between thaum-atin molecules that do not contain cysteine residues. J Agric Food Chem 47, 4950-4955.
33. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
34. Reisfeld RA, Lewis UJ & Williams DE (1962) Disk electrophoresis of basic proteins and peptides on poly-acrylamide gels. Nature 195, 281-283.