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Volumn 12, Issue 5, 2011, Pages 707-710

A Route to Novel Functional Metalloproteins via Hybrids of Cytochrome P450 and Cytochrome c

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME C; CYTOCHROME P450; GUAIACOL; HYBRID PROTEIN;

EID: 79952690828     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.201000631     Document Type: Article
Times cited : (8)

References (25)
  • 8
    • 79952667925 scopus 로고    scopus 로고
    • SRSs are generally conserved among most CYP450s. Here the SRSs of CYP450 2C8, one of the principal drug-metabolizing CYP450 variants expressed in human liver and able to catalyze substrates of relatively large size, were chosen for the construction.
    • SRSs are generally conserved among most CYP450s. Here the SRSs of CYP450 2C8, one of the principal drug-metabolizing CYP450 variants expressed in human liver and able to catalyze substrates of relatively large size, were chosen for the construction.
  • 11
    • 79952681010 scopus 로고    scopus 로고
    • Models of the hybrids were created with the VMD program and energy-minimized with the aid of the NAMD program and then submitted to CASTp with a 1.4 Å probe radius server to identify pockets and cavities in hybrid proteins.
    • Models of the hybrids were created with the VMD program and energy-minimized with the aid of the NAMD program and then submitted to CASTp with a 1.4 Å probe radius server to identify pockets and cavities in hybrid proteins.
  • 13
    • 79952664710 scopus 로고    scopus 로고
    • See the Supporting Information.
    • See the Supporting Information.
  • 15
    • 79952649119 scopus 로고    scopus 로고
    • 2 and then analyzed by GC-MS.
    • 2 and then analyzed by GC-MS.
  • 18
    • 79952656114 scopus 로고    scopus 로고
    • All tests were carried out in Pi buffer (100 mM, pH 6.0) with a stopped-flow apparatus thermostatted at 25°C.
    • All tests were carried out in Pi buffer (100 mM, pH 6.0) with a stopped-flow apparatus thermostatted at 25°C.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.