Differential epithelial and stromal protein profiles in keratoconus and normal human corneas

Experimental Eye Research

Volumn 92, Issue 4, 2011, Pages 282-298

  Joseph, R ^a   Srivastava, O P ^a   Pfister, R R ^b  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b Eye Research Foundation   (United States)

Author keywords

Cornea; Epithelium; Keratoconus; Stroma; Two dimensional difference gel electrophoresis (2D DIGE)

Indexed keywords

ANNEXIN; BETA TUBULIN; CALPAIN; CALPAIN 1; CYTOKERATIN 12; CYTOKERATIN 14; CYTOKERATIN 16; CYTOKERATIN 5; DECORIN; EYE PROTEIN; FERRITIN; HEAT SHOCK PROTEIN; KERATIN TYPE I; KERATOCAN; LACTATE DEHYDROGENASE; LAMIN A; LAMIN C; LIPOCORTIN 2; PHOSPHOGLYCERATE KINASE; PHOSPHOGLYCERATE KINASE 1; PROTEIN 14 3 3; PROTEIN S 100; PROTEOME; PYRUVATE KINASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE) 1; SERUM ALBUMIN; TRANSFORMING GROWTH FACTOR BETA; TRANSKETOLASE; UNCLASSIFIED DRUG; VIMENTIN;

ADULT; ARTICLE; CONTROLLED STUDY; CORNEA EPITHELIUM; CORNEA STROMA; DOWN REGULATION; ELECTROSPRAY MASS SPECTROMETRY; ENZYME REGULATION; HUMAN; HUMAN TISSUE; KERATOCONUS; LIQUID CHROMATOGRAPHY; METABOLIC STRESS; NANOANALYSIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEOMICS; QUANTITATIVE ANALYSIS; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS; TWO DIMENSIONAL GEL ELECTROPHORESIS; UPREGULATION;

ADULT; CHROMATOGRAPHY, LIQUID; CORNEAL STROMA; DOWN-REGULATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EPITHELIUM, CORNEAL; EYE PROTEINS; HUMANS; KERATOCONUS; MIDDLE AGED; PROTEOMICS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TRANSCRIPTION FACTORS; UP-REGULATION;

EID: 79952617664     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2011.01.008     Document Type: Article

References (102)

1. Al Haj Zen A., Lafont A., Durand E., Brasselet C., Lemarchand P., Godeau G., Gogly B. Effect of adenovirus-mediated overexpression of decorin on metalloproteinases, tissue inhibitors of metalloproteinases and cytokines secretion by human gingival fibroblasts. Matrix Biol. 2003, 22:251-258.
2. Atalino S.R., Coskun P., Chwa M., Jordan N., Reddy V., Le K., Wallace D.C., Kenney M.C. Accumulation of mitochondrial DNA damage in keratoconus corneas. Invest. Ophthalmol. Vis. Sci. 2005, 46:1256-1263.
3. Behndig A., Karlsson K., Johansson B.O., et al. Superoxidase isoenzymes in the normal and diseased human corneas. Invest. Ophthalmol. Vis. Sci. 2001, 42:2293-2296.
4. Beissbarth T., Hyde L., Smyth G.K., Job C., Boon W.M., Tan S.S., Scott H.S., Speed T.P. Statistical modeling of sequencing errors in SAGE libraries. Bioinformatics 2004, 20(Suppl. 1):i31-i39.
5. Billings P.C., Whitbeck J.C., Adams C.S., Abrams W.R., Cohen A.J., Engelsberg B.N., Howard P.S., Rosenbloom J. The transforming growth factor-beta-inducible matrix protein (beta)ig-h3 interacts with fibronectin. J. Biol. Chem. 2002, 277:28003-28009.
6. Bredrup C., Knappskog P.M., Majewski J., Rødahl E., Boman H. Congenital stromal dystrophy of the cornea caused by a mutation in the decorin gene. Invest. Ophthalmol. Vis. Sci. 2005, 46:420-426.
7. Bruey J.M., Ducasse C., Bonniaud P., Ravagnan L., Susin S.A., Diaz-Latoud C., Gurbuxani S., Arrigo A.P., Kroemer G., Solary E., et al. Hsp27 negatively regulates cell death by interacting with cytochrome c. Nat. Cell Biol. 2000, 2:645-652.
8. Cai C.X., Birk D.E., Linsenmayer T.F. Ferritin is a developmentally regulated nuclear protein of avian corneal epithelial cells. J. Biol. Chem. 1997, 272:12831-12839.
9. Charette S.J., Lavoie J.N., Lambert H., Landry J. Inhibition of Daxx-mediated apoptosis by heat shock protein 27. Mol. Cell. Biol. 2000, 20:7602-7612.
10. Chen J., Guerriero E., Sado Y., SundarRaj N. Rho-mediated regulation of TGF-beta1- and FGF-2-induced activation of corneal stromal keratocytes. Invest. Ophthalmol. Vis. Sci. 2009, 50:3662-3670.
11. Chen S., Martin C., Maya-Mendoza A., Tang C.W., Lovrić J., Sims P.F., Jackson D.A. Reduced expression of lamin A/C results in modified cell signaling and metabolism coupled with changes in expression of structural proteins. J. Proteome Res. 2009, 8:5196-5211.
12. Chwa M., Atilano S.R., Reddy V., Jordan N., Kim D.W., Kenney M.C. Increased stress-induced generation of reactive oxygen species and apoptosis in human keratoconus fibroblasts. Invest. Ophthalmol. Vis. Sci. 2006, 47:1902-1910.
13. Chwa M., Atilano S.R., Hertzog D., Zheng H., Langberg J., Kim D.W., Kenney M.C. Hypersensitive response to oxidative stress in keratoconus corneal fibroblasts. Invest. Ophthalmol. Vis. Sci. 2008, 49:4361-4369.
14. Critchfield J.W., Calandra A.J., Nesburn A.B., Kenney M.C. Keratoconus I, biochemical studies of normal and keratoconus corneas. Exp. Eye Res. 1988, 46:953-963.
15. Daxer A., Misof K., Grabner B., Ettl A., Fratzl P. Collagen fibrils in the human corneal stroma: structure and aging. Invest. Ophthalmol. Vis. Sci. 1998, 39:644-843.
16. De Luca A., Santra M., Baldi A., Giordano A., Iozzo R.V. Decorin-induced growth suppression is associated with up-regulation of p21, an inhibitor of cyclin-dependent kinases. J. Biol. Chem. 1996, 271:18961-18965.
17. Dickey L.F., Sreedharan S., Theil E.C., Didsbury J.R., Wang Y.H., Kaufman R.E. Differences in the regulation of messenger RNA for housekeeping and specialized-cell ferritin. A comparison of three distinct ferritin complementary DNAs, the corresponding subunits, and identification of the first processed in amphibia. J. Biol. Chem. 1987, 262:7901-7907.
18. Domon B., Aebersold R. Mass spectrometry and protein analysis. Science 2006, 312:212-217.
19. Fullerton J., Paprocki P., Forrest S. Identity-by-descent approach to gene localization in eight individuals affected by keratoconus from north-west Tasmanaia, Australia. Hum. Gene 2002, 110:462-470.
20. Funderburgh J.L., Funderburgh M.L., Mann M.M., Prakash S., Conrad G.W. Synthesis of corneal keratan sulfate proteoglycans by bovinekeratocytes in vitro. J. Biol. Chem. 1996, 271:31431-31436.
21. Funderburgh J.L., Hevelone N.D., Roth M.R., Funderburgh M.L., Rodrigues M.R., Nirankari V.S., Conrad G.W. Decorin and biglycan of normal and pathologic human corneas. Invest. Ophthalmol. Vis. Sci. 1998, 39:1957-1964.
22. Gass J.D.M. The iron lines of the superficial cornea; Hudson-Stähli line Stocker's line and Fleischer's ring. Arch. Ophthalmol. 1964, 71:348-358.
23. Goldoni S., Owens R.T., McQuillan D.J., et al. Biologically active decorin is a monomer in solution. J. Biol. Chem. 2004, 279:6606-6612.
24. Gondhowiardjo T.D., van Haeringen N.J., Volker-Dieben H.J. Corneal aldehyde dehydrogenase, glutathione reductase, and glutathione S-transferase in pathological corneas. Cornea 1993, 12:310-314.
25. Halliwell B., Gutteridge J.M.C. Free Radicals in Biology and Medicine 2007, Clarendon Press, Oxford. fourth ed.
26. Hartl F.U. Molecular chaperones in cellular protein folding. Nature 1996, 381:571-579.
27. Hayashida-Hibino S., Watanabe H., Tano Y. The Effect of TGF-β1 on differential gene expression profiles in human corneal epithelium studied by cDNA expression array, Invest. Ophthalmol.Vis. Sci. 2001, 42:1691-1697.
28. Hayes M.J., Moss S.E. Annexins and disease. Biochem. Biophys. Res. Commun. 2004, 322:1166-1170.
29. Hayes M.J., Moss S.E. Annexin 2 has a dual role as regulator and effector of v-Src in cell transformation. J. Biol. Chem. 2009, 284:10202-10210.
30. Hiratsuka Y., Nakayasu K., Kanai A. Secondary keratoconus with corneal epithelial iron ring similar to Fleischer's ring. Jpn. J. Ophthalmol. 2000, 44:381-386.
31. Hollinsworth J.G., Efron H., Tullo A.B. In vivo corneal confocal microscopyin keratoconus. Ophthalmic Physiol. Opt. 2005, 25:254-260.
32. Jongebloed W.L., Worst J.F. The keratoconus epithelium studied by SEM. Doc. Ophthalmol. 1987, 67:171-181.
33. Kaldaawy R.M., Wagner J., Chig S., Seigel G.M. Evidence of apotoptotic death in keratoconus. Cornea 2002, 2:206-209.
34. Kao W.W., Velegnes J.P., Ebert J., Sunder-Raj C.V., Brown S.I. Increased collagenase and gelatinase activities in keratoconus. Biochem. Biophys. Res. Commun. 1982, 107:929-936.
35. Karring H., Thøgersen I.B., Klintworth G.K., Møller-Pedersen T., Enghild J.J. A dataset of human cornea proteins identified by peptide mass fingerprinting and tandem mass spectrometry. Mol. Cell Proteomics 2005, 4:1406-1408.
36. Karring H., Thøgersen I.B., Klintworth G.K., Møller-Pedersen T., Enghild J.J. The human cornea proteome: bioinformatic analyses indicate import of plasma proteins into the cornea. Mol. Vis. 2006, 12:451-460.
37. Kays W.T., Piatigorsky J. Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic mice. Proc. Natl. Acad. Sci. U. S. A. 1997, 94:13594-13599.
38. Keller A., Nesvizhskii A.I., Kolker E., Aebersold R. Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal. Chem. 2002, 74:5383-5392.
39. Kennedy R.H., Bourne W.M., Dyer J.A. A 48-year old clinical epidemiological study of keratoconus. Am. J. Ophthalmol. 1986, 10:267-273.
40. Kenney M.C., Nesburn A.B., Burgeson R.E., Butkowski R.J., Ljubimov A.V. Abnormalities of the extracellular matrix in keratoconus corneas. Cornea 1997, 16:345-351.
41. Kenney M.C., Brown D.J., Rajeev B. Evrett Kinsey lecture: the elusive cause of keratoconus, a working hypothesis. CLAO J. 2000, 26:10-13.
42. Kenney M.C., Chwa M., Atilano S.R., Tran A., Carballo M., Saghizadeh M., Vasiliou V., Adachi W., Brown D.J. Increased levels of catalase and cathepsin V/L2 but decreased TIMP-1 in keratoconus corneas: evidence that oxidative stress plays a role in this disorder. Invest. Ophthalmol. Vis. Sci. 2005, 46:823-832.
43. Kim W.J., Rabinowitz Y.S., Meisler D.M., Wilson S.E. Keratocytes apoptosis associated with keratoconus. Exp. Eye Res. 1999, 69:475-481.
44. Ku J.Y., Niederer R.L., Patel D.V., et al. Laser scanning in vivo confocal analysis of keratocytes density in keratoconus. Ophthalmology 2008, 115:845-850.
45. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
46. Lawless M., Coster D.J., Phillips A.J., Loane M. Keratoconus: diagnosis and management. Aust. N. Z. J. Ophthalmol. 1989, 17:33-60.
47. Li D., Qi Y., Wang L., Lin H., Zhou N., Zhao L. An atypical phenotype of Reis-Bücklers corneal dystrophy caused by the G623D mutation in TGFBI. Mol. Vis. 2008, 14:1298-1302.
48. Liu H., Sadygov R.G., Yates J.R. A model for random sampling and estimation of relative protein abundance in shotgun proteomics. Anal. Chem. 2004, 76:193-201.
49. Liu Y., Peng X., Tan J., Darling D.S., Kaplan H.J., Dean D. Zeb1 mutant mice as a model of posterior corneal dystrophy. Invest. Ophthalmol. Vis. Sci. 2008, 49:1843-1849.
50. Lu H., Zimek A., Chen J., Hesse M., Büssow H., Weber K., Magin T.M. Keratin 5 knockout mice reveal plasticity of keratin expression in the corneal epithelium. Eur. J. Cell Biol. 2006, 85:803-811.
51. Markmann A., Hausser H., Schönherr E., Kresse H. Influence of decorin expression on transforming growth factor-β-mediated collagen gel retraction and biglycan induction. Matrix Biol. 2000, 19:631-636.
52. Matthews F.J., Cook S.D., Majid M.A., Dick A.D., Smith V.A. Changes in the balance of the tissue inhibitor of matrix metalloproteinases (TIMPs)-1 and -3 may promote keratocyte apoptosis in keratoconus. Exp. Eye Res. 2007, 84:1125-1133.
53. Michelacci Y.M. Collagens and proteoglycans of the corneal extracellular matrix. Braz. J. Med. Biol. Res. 2003, 36:1037-1046.
54. Miura T., Kishioka Y., Wakamatsu J., Hattori A., Hennebry A., Berry C.J., Sharma M., Kambadur R., Nishimura T. Decorin binds myostatin and modulates its activity to muscle cells. Biochem. Biophys. Res. Commun. 2006, 340:675-680.
55. Mohan R.R., Gupta R., Mehan M.K., Cowden J.W., Sinha S. Decorin transfection suppresses profibrogenic genes and myofibroblast formation in human corneal fibroblasts. Exp. Eye Res. 2010, 91:238-245.
56. Munier F.L., Korvatska E., Djemai A., Le Paslier D., Zografos L., Pescia G., Schorderet D.F. Kerato-epithelin mutations in four 5q31-linked corneal dystrophies. Nat. Genet. 1997, 15:247-251.
57. NIederer R.L., Perumal D., Sherwin T., McGhee C.J. Laser scanning in vivo confocal microscopy reveals reduced innervation and reduction in cell density in all layers of keratoconic corneas. Invest. Ophthalmol. Vis. Sci. 2008, 49:2964-2970.
58. Nesvizhskii A.I., Keller A., Kolker E., Aebersold R. A statistical model for identifying proteins by tandem mass spectrometry. Anal. Chem. 2003, 75:4646-4658.
59. Nielsen K., Birkenkamp-demtrouder K., Eblers N., Orntoft T.F. Identification of differentially expressed genes in keratoconus epithelium genes in keratoconus epithelium analyzed by microarrays. Invest. Ophthalmol. Vis. Sci. 2003, 44:2466-2476.
60. Nielsen K., Vorum H., Fagerholm P., Birkenkamp-Demtroder K., Honore B., Ehlers N., Orntoft T.F. Proteome profiling of corneal epithelium and identification of marker proteins for keratoconus, a pilot. Exp. Eye Res. 2006, 82:201-209.
61. Nioi P., Hayes J.D. Contribution of NAD(P)H:quinone oxidoreductase 1 to protection against carcinogenesis, and regulation of its gene by the Nrf2 basic-region leucine zipper and the arylhydrocarbon receptor basic helix-loop-helix transcription factors. Mutat. Res. 2004, 555:149-171.
62. Nishida K., Kawasaki S., Adachi W., Kinoshita S. Apolipoprotein J expression in human ocular surface epithelium. Invest. Ophthalmol. Vis. Sci. 1996, 37:2285-2292.
63. Old W.M., Meyer-Arendt K., Aveline-Wolf L., Pierce K.G., Mendoza A., Sevinsky J.R., Resing K.A., Ahn N.G. Comparison of label-free methods for quantifying human proteins by shotgun proteomics. Mol. Cell Proteomics 2005, 4:1487-1502.
64. Orge J.P., Irving T.C., Miller A., Wess T.J. Proc. Natl. Acad. Sci. U. S. A. 2006, 103:9001-9005.
65. Pappa A., Estey T., Manzer R., Brown D., Vasiliou V. Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea. Biochem. J. 2003, 15:615-623.
66. Patel D.V., McGhee C.N.J. Mapping of corneal sub-basal nerve plexus in keratoconus by an in vivo laser scanning confocal microscope. Invest. Ophthalmol. Vis. Sci. 2006, 47:1348-1351.
67. Patel S., McLaughlin J.M. Effects of central corneal thickness on measurement of intra-ocular pressure in keratoconus and post-keratoplasty. Ophthalmic Physiol. Opt. 1999, 19:236-241.
68. Perng M.D., Cairns L., Van den I.P., Prescott A., Hutcheson A.M., Quinlan R.A. Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin. J. Cell Sci. 1999, 112:2099-2112.
69. Pfister R.R., Burstein N. The normal and abnormal Human corneal epithelial surface: a scanning electron microscope study. Invest. Ophthalmol. Vis. Sci. 1977, 16:614-626.
70. Polack F.M. Contributions of electron microscopy to the study of corneal pathology. Surv. Ophthalmol. 1976, 20:375-414.
71. Rabinowitz Y.S. The genetics of keratoconus. Ophthalmol. Clin. North Am. 2003, 16:607-620.
72. Rabinowitz Y.S., Dong L., Wistow G. Gene expression profile studies of human keratoconus cornea for NEIBank: a novel cornea-expressed gene and the absence of transcripts for aquaporin 5. Invest. Ophthalmol. Vis. Sci. 2005, 46:1239-1246.
73. Rehany U., Lahau M., Shoshau S. Collagenolytic activity in keratoconus. Ann. Ophthalmol. 1982, 14:751-754.
74. Rock M.E., Moore M.N., Anderson J.A., Binder P.S. 3D-computer models of human keratocytes. CLAO J. 1995, 21:57-60.
75. Rose G., Lavin M. The Hudson-Stahli line III: observations on morphology, a critical review of aetiology and a unified theory for the formation of iron lines of the corneal epithelium. Eye 1987, 1:475-479.
76. Santra M., Skorski T., Calabretta B., Lattime E.C., Iozzo R.V. Proc. Natl. Acad. Sci. U. S. A. 1995, 92:7016-7020.
77. Sawaguchi S., Yue B.Y., Sugar J., Gilboy J.E. Lysosomal enzymes abnormalities in keratoconus. Arch. Opthalmol. 1989, 107:1507-1510.
78. Sawaguchi S., Twinning S.S., Yue B.Y., Wison P.M., Sugar J., Chan S.-K. α1-protease inhibitor levels in normal human and keratoconus corneas. Exp. Eye Res. 1990, 50:549-554.
79. Sawaguchi S., Twinning S.S., Yue B.Y., et al. α1-macroglobulin levels in normal human and keratoconus corneas. Invest. Ophthalmol. Vis. Sci. 1994, 35:4008-4014.
80. Sax C.M., Salamon C., Kays W.T., Guo J., Yu F.X., Cuthbertson R.A., Piatigorsky J. Transketolase is a major protein in the mouse cornea. J. Biol. Chem. 1996, 271:33568-33574.
81. Sax C.M., Kays W.T., Salamon C., Chervenak M.M., Xu Y.S., Piatigorsky J. Transketolase gene expression in the cornea is influenced by environmental factors and developmentally controlled events. Cornea 2000, 19:833-841.
82. Scroggs M.W., Proia A.D. Histopathological variation in keratoconus. Cornea 1992, 11:553-559.
83. Simo Mannion L., Tromans C., O'Donnels C. An evaluation of corneal nerve morphology and function in moderate keratoconus. Cont. Lens Anterior Eye 2005, 28:185-192.
84. Smith V.A., Matthews F.J., Majid M.A., Cook S.D. Keratoconus: matrix metalloproteinase-2 activation and TIMP modulation. Biochim. Biophys. Acta 2006, 1762:431-439.
85. Srivastava O.P., Chandrasekaran D., Pfister R.R. Molecular changes in selected proteins in human keratoconus corneas compared to normal corneas. Mol. Vis. 2006, 12:1615-1625.
86. Stancovski I., Baltimore D. NF-kappaB activation: the I kappaB kinase revealed?. Cell 1997, 91:299-302.
87. Stohs S.J., Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic. Biol. Med. 1995, 18:321-336.
88. Teng C.C. Electron microscopic study of the pathology of keratoconus. Am. J. Ophthalmol. 1963, 55:18-47.
89. Treffry A., Harrison P.M. Metal-ion-binding sites and the role of the iron-core in iron(II) uptake by ferritin. Biochem. Soc. Trans. 1980, 8:655-656.
90. Tsubota K., Mashima Y., Murata H., Sato N., Ogata T. Corneal epithelium in keratoconus. Cornea 1995, 14:77-83.
91. Twining S.S., Fukuchi T., Yue B.Y., Wilson P.M., Boskovic G. Corneal synthesis of alpha 1-proteinase inhibitor (alpha 1-antitrypsin). Invest. Ophthalmol. Vis. Sci. 1994, 35:458-462.
92. Twining S.S., Fukuchi T., Yue B.Y., Wilson P.M., Zhou X. Alpha 1-antichymotrypsin is present in and synthesized by the cornea. Curr. Eye Res. 1994, 13:433-439.
93. US National Eye Institute, Facts About the Cornea and Corneal Disease Keratoconus.
94. Ucakhan O.O., Kanpolat A., Yilmaz N., Ozkan M. In vivo confocal microscopy findings in keratoconus. Eye Contact Lens 2006, 32:183-191.
95. Vasiliou V., Pappa A., Estey T. Role of human aldehyde dehydrogenases in endobiotic and xenobiotic metabolism. Drug Metab. Rev. 2004, 36:279-299.
96. Weatherly D.B., Atwood J.A., Minning T.A., Cavola C., Tarleton R.L., Orlando R. A Heuristic method for assigning a false-discovery rate for protein identifications from Mascot database search results. Mol. Cell Proteomics 2005, 4:762-772.
97. Wentz-Hunter K., Cheng E.L., Ueda J., Sugar J., Yue B.Y. Keratocan expression is increased in the stroma of keratoconus corneas. Mol. Med. 2001, 7:470-477.
98. Wessel H., Anderson S., Fite D., Halvas E., Hempel J., SundarRaj N. Type XII collagen contributes to diversities in human corneal and limbal extracellular matrices. Invest. Ophthalmol. Vis. Sci. 1997, 38:2408-2422.
99. Xin Y., Lu Q., Li Q. 14-3-3 sigma controls corneal epithelial cell proliferation and differentiation through the Notch signaling pathway. Biochem. Biophys. Res. Commun. 2010, 392:593-598.
100. Zhao Y.L., Piao C.Q., Hei T.K. Downregulation of Betaig-h3 gene is causally linked to tumorigenic phenotype in asbestos treated immortalized human bronchial epithelial cells. Oncogene 2002, 21:7471-7477.
101. Zhao Y., Pan X., Zhao J., Wang Y., Peng Y., Zhong C. Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency. J. Neurochem. 2009, 111:537-546.
102. Zhou L., Sawaguchi S., Twining S.S., Sugar J., Feder R.S., Yue B.Y. Expression of degradative enzymes and protease inhibitors in corneas with keratoconus. Invest. Ophthalmol. Vis. Sci. 1998, 39:1117-1124.