Proteome profiling of corneal epithelium and identification of marker proteins for keratoconus, a pilot study

Experimental Eye Research

Volumn 82, Issue 2, 2006, Pages 201-209

  Nielsen, Kim ^a   Vorum, Henrik ^b   Fagerholm, Per ^b   Birkenkamp Demtröder, Karin ^a   Honoré, Bent ^b   Ehlers, Niels ^a   Orntoft, Torben F ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

^b Bygning 170 ^*  (Sweden)

Author keywords

2D gel; Cornea; Keratoconus; Proteomics; SDS PAGE; Silver staining

Indexed keywords

ALPHA ENOLASE; CALVASCULIN; CYTOKERATIN; CYTOKERATIN 12; CYTOKERATIN 3; GELSOLIN; PROTEOME; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CORNEA EPITHELIUM; HUMAN; HUMAN CELL; KERATOCONUS; MASS SPECTROMETRY; MOLECULAR WEIGHT; MYOPIA; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEOMICS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BIOLOGICAL MARKERS; CASE-CONTROL STUDIES; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EPITHELIUM, CORNEAL; EYE PROTEINS; GELSOLIN; GENE EXPRESSION PROFILING; HUMANS; KERATINS; KERATOCONUS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHOSPHOPYRUVATE HYDRATASE; PILOT PROJECTS; PROTEOMICS; S100 PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 31044446857     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2005.06.009     Document Type: Article

References (46)

1. N. Belot, R. Pochet, C.W. Heizmann, R. Kiss, and C. Decaestecker Extracellular S100A4 stimulates the migration rate of astrocytic tumor cells by modifying the organization of their actin cytoskeleton Biochim. Biophys. Acta 1600 2002 74 83
2. F. Brancati, E.M. Valente, A. Sarkozy, J. Feher, M. Castori, P. Del Duca, R. Mingarelli, A. Pizzuti, and B. Dallapiccola A locus for autosomal dominant keratoconus maps to human chromosome 3p14-q13 J. Med. Genet. 41 2004 188 192
3. C. Chaponnier, P.A. Janmey, and H.L. Yin The actin filament-severing domain of plasma gelsolin J. Cell Biol. 103 1986 1473 1481
4. H. Chen, D.G. Fernig, P.S. Rudland, A. Sparks, M.C. Wilkinson, and R. Barraclough Binding to intracellular targets of the metastasis-inducing protein, S100A4 (p9Ka) Biochem. Biophys. Res. Commun. 286 2001 1212 1217
5. E.H. Chung, P.G. DeGregorio, M. Wasson, and J.D. Zieske Epithelial regeneration after limbus-to-limbus debridement. Expression of alpha-enolase in stem and transient amplifying cells Invest. Ophthalmol. Vis. Sci. 36 1995 1336 1343
6. S.A. Collier Is the corneal degradation in keratoconus caused by matrix-metalloproteinases? Clin. Experiment Ophthalmol. 29 2001 340 344
7. C.C. Cunningham, T.P. Stossel, and D.J. Kwiatkowski Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin Science 251 1991 1233 1236
8. A. de la Chapelle, R. Tolvanen, G. Boysen, J. Santavy, L. Bleeker-Wagemakers, C.P. Maury, and J. Kere Gelsolin-derived familial amyloidosis caused by asparagine or tyrosine substitution for aspartic acid at residue 187 Nat. Genet. 2 1992 157 160
9. J. Fullerton, P. Paprocki, S. Foote, D.A. Mackey, R. Williamson, and S. Forrest Identity-by-descent approach to gene localisation in eight individuals affected by keratoconus from north-west Tasmania, Australia Hum. Genet. 110 2002 462 470
10. B. Futcher, G.I. Latter, P. Monardo, C.S. McLaughlin, and J.I. Garrels A sampling of the yeast proteome Mol. Cell Biol. 19 1999 7357 7368
11. A. Gorg, G. Boguth, C. Obermaier, and W. Weiss Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient Electrophoresis 19 1998 1516 1519
12. S.P. Gygi, Y. Rochon, B.R. Franza, and R. Aebersold Correlation between protein and mRNA abundance in yeast Mol. Cell Biol. 19 1999 1720 1730
13. B. Honore, H. Vorum, A.E. Pedersen, S. Buus, and M.H. Claesson Changes in protein expression in p53 deleted spontaneous thymic lymphomas Exp. Cell Res. 295 2004 91 101
14. M.E. Huff, L.J. Page, W.E. Balch, and J.W. Kelly Gelsolin domain 2 Ca2+ affinity determines susceptibility to furin proteolysis and familial amyloidosis of finnish type J. Mol. Biol. 334 2003 119 127
15. A.E. Hughes, D.P. Dash, A.J. Jackson, D.G. Frazer, and G. Silvestri Familial keratoconus with cataract: linkage to the long arm of chromosome 15 and exclusion of candidate genes Invest. Ophthalmol. Vis. Sci. 44 2003 5063 5066
16. S.R. Jenkinson, R. Barraclough, C.R. West, and P.S. Rudland S100A4 regulates cell motility and invasion in an in vitro model for breast cancer metastasis Br. J. Cancer 90 2004 253 262
17. S. Kamada, H. Kusano, H. Fujita, M. Ohtsu, R.C. Koya, N. Kuzumaki, and Y. Tsujimoto A cloning method for caspase substrates that uses the yeast two-hybrid system: cloning of the antiapoptotic gene gelsolin Proc. Natl. Acad. Sci. USA 95 1998 8532 8537
18. R.H. Kennedy, W.M. Bourne, and J.A. Dyer A 48-year clinical and epidemiologic study of keratoconus Am. J. Ophthalmol. 101 1986 267 273
19. I.A. King, and E.F. Hounsell Cytokeratin 13 contains O-glycosidically linked N-acetylglucosamine residues J. Biol. Chem. 264 1989 14022 14028
20. T. Kivela, and M. Uusitalo Structure, development and function of cytoskeletal elements in non-neuronal cells of the human eye Prog. Retin. Eye Res. 17 1998 385 428
21. M.V. Kriajevska, M.N. Cardenas, M.S. Grigorian, N.S. Ambartsumian, G.P. Georgiev, and E.M. Lukanidin Non-muscle myosin heavy chain as a possible target for protein encoded by metastasis-related mts-1 gene J. Biol. Chem. 269 1994 19679 19682
22. M.A. Kurpakus, E.L. Stock, and J.C. Jones Expression of the 55-kD/64-kD corneal keratins in ocular surface epithelium Invest. Ophthalmol. Vis. Sci. 31 1990 448 456
23. D.J. Kwiatkowski, T.P. Stossel, S.H. Orkin, J.E. Mole, H.R. Colten, and H.L. Yin Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain Nature 323 1986 455 458
24. D.J. Kwiatkowski, R. Mehl, and H.L. Yin Genomic organization and biosynthesis of secreted and cytoplasmic forms of gelsolin J. Cell Biol. 106 1988 375 384
25. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
26. W.M. Lee, and R.M. Galbraith The extracellular actin-scavenger system and actin toxicity N. Engl. J. Med. 326 1992 1335 1341
27. A. Mandinova, D. Atar, B.W. Schafer, M. Spiess, U. Aebi, and C.W. Heizmann Distinct subcellular localization of calcium binding S100 proteins in human smooth muscle cells and their relocation in response to rises in intracellular calcium J. Cell Sci. 14 1998 2043 2054
28. C.P. Maury, J. Kere, R. Tolvanen, and C.A. de la Finnish hereditary amyloidosis is caused by a single nucleotide substitution in the gelsolin gene FEBS Lett. 276 1990 75 77
29. A.M. McGough, C.J. Staiger, J.K. Min, and K.D. Simonetti The gelsolin family of actin regulatory proteins: modular structures, versatile functions FEBS Lett. 552 2003 75 81
30. E. Mortz, T.N. Krogh, H. Vorum, and A. Gorg Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis Proteomics 1 2001 1359 1363
31. K. Nielsen, K. Birkenkamp-Demtroder, N. Ehlers, and T.F. Orntoft Identification of differentially expressed genes in keratoconus epithelium analyzed on microarrays Invest. Ophthalmol. Vis. Sci. 44 2003 2466 2476
32. Nielsen, K., Heegaard, S., Vorum, H., Birkenkamp-Demtroder, K., Ehlers, N., Orntoft, T.F., 2005. Altered expression of CLC, DSG3, EMP3, S100A2, and SLPI in corneal epithelium from keratoconus patients. Cornea August, In press.
33. K. Nishida, W. Adachi, A. Shimizu-Matsumoto, S. Kinoshita, K. Mizuno, K. Matsubara, and K. Okubo A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA Invest. Ophthalmol. Vis. Sci. 37 1996 1800 1809
34. M.B. Omary, N.O. Ku, J. Liao, and D. Price Keratin modifications and solubility properties in epithelial cells and in vitro Harris Hermann Subcellular Biochemistry 1998 Plenum Press New York 105 140
35. N. Panjwani, J. Drysdale, B. Clark, J. Alberta, and J. Baum Protein-related abnormalities in keratoconus Invest. Ophthalmol. Vis. Sci. 30 1989 2481 2487
36. D.N. Perkins, D.J. Pappin, D.M. Creasy, and J.S. Cottrell Probability-based protein identification by searching sequence databases using mass spectrometry data Electrophoresis 20 1999 3551 3567
37. Y.S. Rabinowitz, L. Don, J. Gao, P. Buchoff, and G. Wistow Gene expression profile of keratoconus cornea for NEIBank ARVO 2004 (poster #2883/B518)
38. D.G. Ryan, L. Taliana, L. Sun, Z.G. Wei, S.K. Masur, and R.M. Lavker Involvement of S100A4 in stromal fibroblasts of the regenerating cornea Invest. Ophthalmol. Vis. Sci. 44 2003 4255 4262
39. A. Shevchenko, M. Wilm, O. Vorm, and M. Mann Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels Anal. Chem. 68 1996 850 858
40. A. Subramanian, and D.M. Miller Structural analysis of alpha-enolase. Mapping the functional domains involved in down-regulation of the c-myc protooncogene J. Biol. Chem. 275 2000 5958 5965
41. K. Takenaga, Y. Nakamura, S. Sakiyama, Y. Hasegawa, K. Sato, and H. Endo Binding of pEL98 protein, an S100-related calcium-binding protein, to nonmuscle tropomyosin J. Cell Biol. 124 1994 757 768
42. H. Tyynismaa, P. Sistonen, S. Tuupanen, T. Tervo, A. Dammert, T. Latvala, and T. Alitalo A locus for autosomal dominant keratoconus: linkage to 16q22.3-q23.1 in Finnish families Invest. Ophthalmol. Vis. Sci. 43 2002 3160 3164
43. H. Vorum, P. Madsen, I. Svendsen, J.E. Cells, and B. Honore Expression of recombinant psoriasis-associated fatty acid binding protein in Escherichia coli: gel electrophoretic characterization, analysis of binding properties and comparison with human serum albumin Electrophoresis 19 1998 1793 1802
44. J.S. Wang, J.P. Coburn, A.I. Tauber, and K.S. Zaner Role of gelsolin in actin depolymerization of adherent human neutrophils Mol. Biol. Cell 8 1997 121 128
45. L.A. Williams, L. Ding, J. Horwitz, and J. Piatigorsky tau-Crystallin from the turtle lens: purification and partial characterization Exp. Eye Res. 40 1985 741 749
46. J.D. Zieske, G. Bukusoglu, and M.A. Yankauckas Characterization of a potential marker of corneal epithelial stem cells Invest. Ophthalmol. Vis. Sci. 33 1992 143 152