Purification of singly PEGylated α-lactalbumin using charged ultrafiltration membranes

Biotechnology and Bioengineering

Volumn 108, Issue 4, 2011, Pages 822-829

  Ruanjaikaen, Krisada ^a   Zydney, Andrew L ^a  

^a The Pennsylvania State University   (United States)

Author keywords

A lactalbumin; Diafiltration; Downstream processing; PEGylation; Polyethylene glycol; Protein purification; Ultrafiltration

Indexed keywords

A-LACTALBUMIN; DIAFILTRATION; DOWNSTREAM-PROCESSING; PEGYLATION; PROTEIN PURIFICATION;

COMPOSITE MEMBRANES; IONIC STRENGTH; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; POLYETHYLENES; PROTEINS; THERMOPLASTICS; ULTRAFILTRATION;

PURIFICATION;

ALPHA LACTALBUMIN;

ARTICLE; CELL MEMBRANE; DIAFILTRATION; FEASIBILITY STUDY; IONIC STRENGTH; PH; PROTEIN PURIFICATION; STATIC ELECTRICITY; ULTRAFILTRATION;

LACTALBUMIN; MEMBRANES, ARTIFICIAL; OSMOLAR CONCENTRATION; POLYETHYLENE GLYCOLS; STATIC ELECTRICITY; ULTRAFILTRATION;

EID: 79952608234     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22991     Document Type: Article

References (32)

1. Bailon P, Palleroni A, Schaffer CA, Spence CL, Fung WJ, Porter JE, Ehrlich GK, Pan W, Xu ZX, Modi MW, Farid A, Berthold W. 2001. Rational design of a potent, long-lasting form of interferon: A 40-kDa branched polyethylene glycol-conjugated interferon alpha-2a for the treatment of hepatitis C. Bioconjugate Chem 12: 195-202.
2. Basu A, Yang K, Wang ML, Liu S, Chintala R, Palm T, Zhao H, Peng P, Wu DC, Zhang ZF, Hua J, Hsieh MC, Zhou J, Petti G, Li XG, Janjua A, Mendez M, Liu J, Longley C, Zhang Z, Mehlig M, Borowski V, Viswanathan M, Filpula D. 2006. Structure-function engineering of interferon-beta-1b for improving stability, solubility, potency, immunogenicity, and pharmacokinetic properties by site-selective mono-PEGylation. Bioconjugate Chem 17(3): 618-630.
3. Browne WJ, North ACT, Phillips DC. 1969. A possible three-dimensional structure of bovine α-lactalbumin based on that of hen's egg-white lysozyme. J Mol Biol 42: 65-86.
4. Burns DB, Zydney AL. 2000. Buffer effects on the zeta potential of ultrafiltration membranes. J Membr Sci 2000, 172: 39-48.
5. Fee CJ, Van Alstine JM. 2004. Prediction of the viscosity radius and the size exclusion chromatography behavior of PEGylated proteins. Bioconjugate Chem 15: 1304-1313.
6. Fee CJ, Van Alstine JM. 2006. PEG-proteins: Reaction engineering and separation issues. Chem Eng Sci 61: 924-939.
7. Gaberc-Porekar V, Zore I, Podobnik B, Menart V. 2008. Obstacles and pitfalls in PEGylation of therapeutic proteins. Curr Opin Drug Discov Devel 11: 242-250.
8. Grace M, Youngster S, Gitlin G, Sydor W, Xie L, Westreich L, Jacobs S, Brassard D, Bausch J, Bordens R. 2001. Structural and biologic characterization of pegylated recombinant IFN-alpha 2b. J Interferon Cytokine Res 21: 1103-1115.
9. He XH, Shaw PC, Tam SC. 1999. Reducing the immunogenicity and improving the in vivo activity of trichosanthin by site-directed PEGylation. Life Sci 65: 355-368.
10. Kinstler OB, Brems DN, Lauren SL, Paige AG, Hamburger JB, Treuheit MJ. 1996. Characterization and stability of N-terminally PEGylated rhG-CSF. Pharm Res 13: 996-1002.
11. Kwon B, Molek JR, Zydney AL. 2008. Ultrafiltration of PEGylated proteins: Fouling and concentration polarization effects. J Membr Sci 319: 206-213.
12. Lee DL, Sharif I, Kodihalli S, Stewart DIH, Tsvetnitsky V. 2008. Preparation and characterization of monopegylated human granulocyte-macrophage colony-stimulating factor. J Interferon Cytokine Res 28: 101-112.
13. Lee H, Park TG. 2002. Preparation and characterization of mono-PEGylated epidermal growth factor: Evaluation of in vitro biologic activity. Pharm Res 19: 845-851.
14. Lee KC, Moon SC, Park MO, Lee JT, Na DH, Yoo SD, Lee HS, DeLuca PP. 1999. Isolation, characterization, and stability of positional isomers of mono-PEGylated salmon calcitonins. Pharm Res 16: 813-818.
15. Molek JR, Ruanjaikaen K, Zydney AL. 2010. Effect of electrostatic interactions on transmission of PEGylated proteins through charged ultrafiltration membranes. J Membr Sci 353: 60-69.
16. Molek JR, Zydney AL. 2006. Ultrafiltration characteristics of pegylated proteins. Biotechnol Bioeng 95: 474-482.
17. Molek JR, Zydney AL. 2007. Separation of PEGylated α-lactalbumin from unreacted precursors and byproducts using ultrafiltration. Biotechnol Prog 23: 1417-1424.
18. Moosmann A, Christel J, Boettinger H, Mueller E. 2010. Analytical and preparative separation of PEGylated lysozyme for the characterization of chromatography media. J Chromatogr A 1217: 209-215.
19. Morar AS, Schrimsher JRL, Chavez MD. 2006. PEGylation of proteins: Structural approach. Biopharm Int 19: 34-48.
20. Pabst TM, Buckley JJ, Hunter AK. 2007. Comparison of strong anion-exchangers for the purification of a PEGylated protein. J Chromatogr A 1147: 172-182.
21. Piquet G, Gatti M, Barbero L, Traversa S, Caccia P, Esposito P. 2002. Set-up of large laboratory-scale chromatographic separations of poly(ethylene glycol) derivatives of the growth hormone-releasing factor1-29 analogue. J Chromatogr A 944: 141-148.
22. Sato H. 2002. Enzymatic procedure for site-specific pegylation of proteins. Adv Drug Deliv Rev 54: 487-504.
23. Seely JE, Richey CW. 2001. Use of ion-exchange chromatography and hydrophobic interaction chromatrography in the preparation and recovery of polyethylene glycol-linked proteins. J Chromatogr A 908: 235-241.
24. Sehon AH. 1991. Suppression of antibody responses by conjugates of antigens and monomethoxypoly(ethylene glycol). Adv Drug Deliv Rev 6: 203-217.
25. Smith M. 1970. Molecular weights of proteins and some other materials including sedimentation, diffusion and frictional coefficients and partial specific volumes. In: Handbook of Biochemistry, 2nd edn, Cleveland: The Chemical Rubber, Co. p. C3-C292.
26. Stoner MR, Fischer N, Nixon L, Buckel S, Benke M, Austin F, Randolph TW, Kendrick BS. 2004. Protein-solute interactions affect the outcome of ultrafiltration/dialfiltration operations. J Pharm Sci 93: 2332-2342.
27. van Reis R. 2006. Charged Filtration Membranes and Uses Therefore, U.S. 7153, 426.
28. van Reis R, Saksena S. 1997. Optimization diagram for membrane separations. J Membr Sci 129: 19-29.
29. van Reis R, Zydney AL. 2007. Bioprocess membrane technology. J Membr Sci 297: 16-50.
30. Viaene A, Volckaert G, Joniau M, De Baetselier A, van Cauwelaert F. 1991. Efficient expression of bovine alpha-lactalbumin in Saccharomyces cerevisiae Eur J Biochem 202: 471-477.
31. Wang JH, Tam SC, Huang H, Ouyang DY, Wang YY, Zheng YT. 2004. Site-directed PEGylation of trichosanthin retained its anti-HIV activity with reduced potency in vitro. Biochem Biophys Res Commun 317: 965-971.
32. Yun Q, Yang RE, Chen T, Bi JX, Ma GH, Su ZG. 2005. Reproducible preparation and effective separation of PEGylated recombinant human granulocyte colony-stimulating factor with novel PEG-pellet PEGylation mode and ion-exchange chromatography. J Biotechnol 118: 67-74.