Characterization and stability of N-terminally PEGylated rhG-CSF

Pharmaceutical Research

Volumn 13, Issue 7, 1996, Pages 996-1002

  Kinstler, Olaf B ^a   Brems, David N ^a   Lauren, Scott L ^a   Paige, Amiee G ^a   Hamburger, James B ^a   Treuheit, Michael J ^a  

^a AMGEN INC   (United States)

Author keywords

Granulocyte colony stimulating factor (G CSF); N terminal; PEGylation; Site specific; Stability

Indexed keywords

MACROGOL; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR;

ARTICLE; CIRCULAR DICHROISM; DERIVATIZATION; DRUG CONJUGATION; DRUG STABILITY; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID; PEPTIDE MAPPING; PRIORITY JOURNAL;

GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT; POLYETHYLENE GLYCOLS;

EID: 0029759584     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016042220817     Document Type: Article

References (15)

1. D. Metcalf. The Molecular Biology and Functions of the Granulocyte-Macrophage Colony-Stimulating Factor. Blood 67:257-267 (1986).
2. K. Welte, E. L. Platzer, H. Lu, J. Gabrilove, R. Mertelsman, and M. A. S. Moore. Purification and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-Stimulating Factor. Proc. Natl. Acad. Sci. USA 82:1526-1530 (1985).
3. L. M. Souza, T. C. Boone, J. Gabrilove, P. H. Lai, K. M. Zsebo, D. C. Murdock, V. R. Chazin, J. Bruszewski, H. Lu, K. K. Chen, J. Barendt, E. Platzer, M. A. S. Moore, R. Mertlesmann, and K. Weite. Recombinant Human Granulocyte Colony-Stimulationg Factor: Effects on Normal and Leukemic Myeloid Cells. Science 232:61-65 (1986).
4. A. M. Cohen, K. M. Zsebo, H. Inoue, D. Hines, T. C. Boone, V. R. Chazin, L. Tsai, T. Ritch, and L. M. Souza. In Vivo Stimulation of Granulopoiesis by Recombinant Human Granulocyte Colony-Stimulating Factor. Proc. Natl. Acad. Sci. USA 84:2484-2488 (1987).
5. Y. Tsutsumi, T. Kihira, S. Tsunoda, N. Okada, Y. Kaneda, Y. Ohsugi, M. Miyake, S. Nakagawa, and T. Mayumi. Polyethylene Glycol Modification of Interleukin-6 Enhances its Thrombopoietic Activity. J. Controlled Release 33:447-451 (1995).
6. H. F. Gaertner and R. E. Offord. Site-Specific Attachment of Functionalized Poly(Ethylene Glycol) to the Amino Terminus of Proteins. Bioconjugate Chem. 7:38-44 (1996).
7. Y. Inada, M. Furukawa, H. Sasaki, Y. Kodera, M. Hiroto, H. Nishimura, and A. Matsushima. Trends Biotechnol. 13:86-91 (1995).
8. S. Davis, A. Abuchowski, Y. K. Park, and F. F. Davis. Alteration of the Circulating Life and Antigenic Properties of Bovine Adenosine Deaminase in Mice by Attachment of Polyethylene Glycol. Clin. Exp. Immunol. 46:649-652 (1981).
9. O. B. Kinstler. N-Terminally Chemically Modified Protein Composition, and Methods. U.S. Patent Serial No. 08/321,510 (1994).
10. S. S. Wong. Chemistry of Protein Conjugation and Cross-Linking, CRC Press, Boca Raton, 1991.
11. U. K. Laemmli. Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4. Nature 227:680-685.
12. T. L. Kirley. Reduction and Fluorescent Labeling of Cyst(e)ine-Containing Proteins for Subsequent Structural Analyses. Anal. Biochem. 180:231-236 (1989).
13. M. M. Kurfurst. Detection and Molecular Weight Determination of Polyethylene Glycol-Modified Hirudin by Staining after Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Anal. Biochem. 200:244-248 (1992).
14. R. Satake-Ishikawa, M. Ishikawa, Y. Okada, M. Kakitani, M. Kawagishi, S. Matsuki, and K. Asano. Chemical Modification of Recombinant Human Granulocyte Colony-Stimulating Factor by Polyethylene Glycol Increases its Biological Activity In Vivo. Cell Structure and Function 17:157-160 (1992).
15. M. J. Knauf, D. P. Bell, P. Hirtzer, Z -P. Luo, J. D. Young, and N. V. Katre. Relationship of Effective Molecular Size to Systemic Clearance in Rats of Recombinant Interleukin-2 Chemically Modified with Water-Soluble Polymers. JBC 263:15064-15070 (1988).