The protein hydrolysate, Primatone RL, is a cost-effective multiple growth promoter of mammalian cell culture in serum-containing and serum-free media and displays anti-apoptosis properties

Journal of Immunological Methods

Volumn 194, Issue 2, 1996, Pages 191-199

  Schlaeger, Ernst Jürgen ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

apoptosis; cell culture; hybridoma; monoclonal antibody; primatone RL; serum free

Indexed keywords

GROWTH PROMOTOR; MONOCLONAL ANTIBODY;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CULTURE MEDIUM; HYBRIDOMA; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; INSECTA; MAMMALIA;

EID: 0030583232     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00080-4     Document Type: Article

References (23)

1. Amborski, R.L. and Moskowitz, M. (1968) The effects of low molecular weight materials derived from animal tissues on the growth of animal cells in vitro. Exp. Cell Res. 53, 117-128.
2. Brockhaus, M., Schoenfeld, H.-J., Schlaeger, E.-J., Hunziker, W., Lesslauer, W. and Loetscher H. (1990) Proc. Natl. Acad. Sci. USA 87, 3127-3131.
3. Glassy, M.C., Tharakan, J.P. and Chau, P.C. (1988) Serum-free media in hybridoma culture and monoclonal antibody production. Biotechnol. Bioeng. 32, 1015-1028.
4. Hansen, H.A. and Emborg, C. (1992) Complex medium supplements give difficulties when investigating mammalian cell physiology. In: R.E. Spier, J.B. Griffith and C. MacDonald (Eds.), Animal Cell Technology, Developments, Processes and Products. Butterworth-Heinemann, Oxford, pp. 248-250..
5. Hink, W.F. (1970) Established cell line from the cabbage looper, Trichoplusia ni. Nature 226, 466-467.
6. Iscove, N.N. (1984) Culture of lymphocytes and hemopoietic cells in serum-free medium. In: D.W. Barnes, D.A. Sirbascu and G.H. Sato (Eds.), Cell Culture Methods for Molecular and Cell Biology, Vol. 4, Methods for Serum-Free Culture of Neuronal and Lymphoid Cells. Liss, New York, pp. 169-185.
7. Jan, D.C.-H., Jones, S.J., Emery, A.N. and Al-Rubeai, M. (1994) Peptone, a low cost growth-promoting nutrient for intensive animal cell culture. Cytotechnology 16, 17-26.
8. Mitsuhashi, J. and Maramorosch, K. (1964) Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thomson Inst. 22, 435-460.
9. Mizrahi, A. (1977) Primatone RL in mammalian cell culture media. Biotechnol. Bioeng. 19, 1557-1561.
10. Patterson, M.K. (1979) Measurement of growth and viability of cells in culture. Methods Enzymol. 58, 141-152.
11. Schlaeger, E.-J. (1996) Medium design for insect cell culture. In: J.M. Vlak, C.D. Gooijer, H.G. Miltenburger and J. Tramper (Eds.), Insect cell culture: fundamental and applied aspects [special issue]. Cytotechnology, in press.
12. Schlaeger, E.-J. and Christensen, K. (1995) Improvement of mammalian fed-batch culture. In: R.E. Spier, J.B. Griffiths and C. Beuvery (Eds.), Animal Cell Technology, Developments Towards the 21st Century. Kluwer Academic Publishers, Dordrecht, in press.
13. Schlaeger, E.-J. and Schumpp, B. (1992) Propagation of a mouse myeloma cell line J558L producing human immunoglobulin G1. J. Immunol. Methods 146, 111-120.
14. Schlaeger, E.-J. and Simpson, S. D. (1994) Cell death (apoptosis) in cell culture. In: Animal Cell Technology ' Products of Today, Prospects for Tomorrow', Eds. Spier, R.E., Griffiths, J.B. and Beuvery, C. Butterworth-Heinemann, Oxford, pp. 183-186.
15. Schlaeger, E.-J., Foggetta, M., Vonach, J.M. and Christensen, K. (1993) SF-1, a low cost culture medium for the production of recombinant proteins in baculovirus infected insect cells. Biotechnol. Tech. 7, 183-188.
16. Schumpp, B. and Schlaeger, E.-J. (1990) Optimization of culture conditions for high cell density proliferation of HL-60 human leukemia cells. J. Cell Sci. 97, 639-647.
17. Shacter, E. (1989) Serum-free media for bulk culture of hybridoma cells and the preparation of monoclonal antibodies. TibTech 7, 248-253.
18. Sheffield Series (1987) The Fermentation and Diagnostic Media. Quest International.
19. Sheffield Series (1993) Hydrolyzed Proteins - Yeast Specialties. Technical Manual. Quest International
20. Velez, D., Reuveny, S., Miller, L. and Macmillan, J.D. (1986) Kinetics of monoclonal antibody production in low serum growth medium. J. Immunol. Methods 86, 45-52.
21. Wyllie, A.H. (1992) Apoptosis and the regulation of cell numbers in normal neoplastic tissues: an over view. Cancer Metastas. Rev. 11, 95-103.
22. Yamane, I. and Murakami, O. (1973) 6,8-Dihydroxypurine: A novel growth factor for mammalian cells in vitro, isolated from a commercial peptone. J. Cell Physiol. 81, 281-284.
23. Zoon, K.C., Bridgen, P.J and Smith, M.E. (1979) Production of human lymphoblastoid interferon by Namalwa cells cultured in serum-free media. J. Gen. Virol. 44, 227-229.