High-throughput screening of microscale pitted substrate topographies for enhanced nonviral transfection efficiency in primary human fibroblasts

Biomaterials

Volumn 32, Issue 14, 2011, Pages 3611-3619

  Adler, Andrew F ^a   Speidel, Alessondra T ^a   Christoforou, Nicolas ^a   Kolind, Kristian ^b   Foss, Morten ^b   Leong, Kam W ^a  

^a Duke University   (United States)

^b AARHUS UNIVERSITY   (Denmark)

Author keywords

Cell spreading; Combinatorial screening; Fibroblast; Gene transfer; Microstructure; Surface topography

Indexed keywords

CELL PHENOTYPE; CELL SPREADING; COMBINATORIAL LIBRARY; COMBINATORIAL SCREENING; CYTOSKELETAL; HIGH-THROUGHPUT SCREENING; HUMAN DERMAL FIBROBLASTS; HUMAN FIBROBLAST; MICRO-SCALES; MICROPITS; MICROSCALE TOPOGRAPHY; NEW DESIGN; NON-VIRAL GENE DELIVERY; NONVIRAL; PROLIFERATION RATE; PROTEIN EXPRESSIONS; SEM; SMOOTH SUBSTRATES; SUBSTRATE TOPOGRAPHY; TRANSFECTION EFFICIENCY; TRANSFECTION SYSTEM;

CELL CULTURE; CONFOCAL MICROSCOPY; FIBROBLASTS; FLOW CYTOMETRY; GENE THERAPY; GENE TRANSFER; MICROSTRUCTURE; MOLECULAR BIOLOGY; OPTIMIZATION; PRECISION ENGINEERING; SUBSTRATES; TISSUE; TOPOGRAPHY;

SURFACE TOPOGRAPHY;

GREEN FLUORESCENT PROTEIN; LIPOPLEX; LUCIFERASE;

ARTICLE; CELL COUNT; CELL PROLIFERATION; CELL STRUCTURE; CELLULAR PARAMETERS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FIBROBLAST; FLOW CYTOMETRY; GEOMETRY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SUBSTRATE TOPOGRAPHY;

CELL PROLIFERATION; CELLS, CULTURED; FIBROBLASTS; FLOW CYTOMETRY; GENETIC VECTORS; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, SCANNING; TRANSFECTION;

EID: 79952100822     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2011.01.040     Document Type: Article

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