RNAi-mediated knock-down of arylamine N-acetyltransferase-1 expression induces E-cadherin up-regulation and cell-cell contact growth inhibition

PLoS ONE

Volumn 6, Issue 2, 2011, Pages

  Tiang, Jacky M ^a   Butcher, Neville J ^a   Cullinane, Carleen ^b   Humbert, Patrick O ^b   Minchin, Rodney F ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b PETER MACCALLUM CANCER CENTRE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ARYLAMINE ACETYLTRANSFERASE; ARYLAMINE ACETYLTRANSFERASE 1; MESSENGER RNA; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; UVOMORULIN; CADHERIN; ISOENZYME; N ACETYLTRANSFERASE 1; N-ACETYLTRANSFERASE 1; SMALL INTERFERING RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL CONTACT; CELL STRAIN HT29; CELL STRUCTURE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; GROWTH INHIBITION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; RNA INTERFERENCE; STRUCTURE ANALYSIS; TUMOR GROWTH; UPREGULATION; ANIMAL; CELL COMMUNICATION; CELL PROLIFERATION; COLON TUMOR; ENZYMOLOGY; GENE SILENCING; GENETICS; KINETICS; METABOLISM; PATHOLOGY;

ANIMALS; ARYLAMINE N-ACETYLTRANSFERASE; CADHERINS; CELL COMMUNICATION; CELL PROLIFERATION; CELL SURVIVAL; COLONIC NEOPLASMS; FEMALE; GENE KNOCKDOWN TECHNIQUES; HT29 CELLS; HUMANS; ISOENZYMES; KINETICS; MICE; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; UP-REGULATION;

EID: 79951796583     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017031     Document Type: Article

References (39)

1. Hein DW, (2002) Molecular genetics and function of NAT1 and NAT2: role in aromatic amine metabolism and carcinogenesis. Mutat Res 506-507: 65-77.
2. Butcher NJ, Boukouvala S, Sim E, Minchin RF, (2002) Pharmacogenetics of the arylamine N-acetyltransferases. Pharmacogenomics J 2: 30-42.
3. Minchin RF, (1995) Acetylation of p-aminobenzoylglutamate, a folic acid catabolite, by recombinant human arylamine N-acetyltransferase and U937 cells. Biochem J 307 (Pt 1): 1-3.
4. Butcher NJ, Tetlow NL, Cheung C, Broadhurst GM, Minchin RF, (2007) Induction of human arylamine N-acetyltransferase type I by androgens in human prostate cancer cells. Cancer Res 67: 85-92.
5. Butcher NJ, Arulpragasam A, Minchin RF, (2004) Proteasomal degradation of N-acetyltransferase 1 is prevented by acetylation of the active site cysteine- A mechanism for the slow acetylator phenotype and substrate-dependent down-regulation. Journal of Biological Chemistry 279: 22131-22137.
6. Butcher NJ, Ilett KF, Minchin RF, (2000) Substrate-dependent regulation of human arylamine N-acetyltransferase-1 in cultured cells. Mol Pharmacol 57: 468-473.
7. Sorlie T, Perou CM, Tibshirani R, Aas T, Geisler S, et al. (2001) Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications. Proc Natl Acad Sci U S A 98: 10869-10874.
8. Adam PJ, Berry J, Loader JA, Tyson KL, Craggs G, et al. (2003) Arylamine N-acetyltransferase-1 is highly expressed in breast cancers and conveys enhanced growth and resistance to etoposide in vitro. Mol Cancer Res 1: 826-835.
9. Bieche I, Girault I, Urbain E, Tozlu S, Lidereau R, (2004) Relationship between intratumoral expression of genes coding for xenobiotic-metabolizing enzymes and benefit from adjuvant tamoxifen in estrogen receptor alpha-positive postmenopausal breast carcinoma. Breast Cancer Res 6: R252-263.
10. Lapointe J, Li C, Higgins JP, van de Rijn M, Bair E, et al. (2004) Gene expression profiling identifies clinically relevant subtypes of prostate cancer. Proc Natl Acad Sci U S A 101: 811-816.
11. Troester MA, Herschkowitz JI, Oh DS, He X, Hoadley KA, et al. (2006) Gene expression patterns associated with p53 status in breast cancer. BMC Cancer 6: 276.
12. Gustafson CE, Wilson PJ, Lukeis R, Baker E, Woollatt E, et al. (1996) Functional evidence for a colorectal cancer tumor suppressor gene at chromosome 8p22-23 by monochromosome transfer. Cancer Res 56: 5238-5245.
13. Butcher NJ, Arulpragasam A, Pope C, Minchin RF, (2003) Identification of a minimal promoter sequence for the human N-acetyltransferase Type I gene that binds AP-1 (activator protein 1) and YY-1 (Yin and Yang 1). Biochemical Journal 376: 441-448.
14. Yang J, Zhang W, Evans PM, Chen X, He X, et al. (2006) Adenomatous polyposis coli (APC) differentially regulates beta-catenin phosphorylation and ubiquitination in colon cancer cells. J Biol Chem 281: 17751-17757.
15. Nagasawa H, Li CY, Maki CG, Imrich AC, Little JB, (1995) Relationship between radiation-induced G1 phase arrest and p53 function in human tumor cells. Cancer Res 55: 1842-1846.
16. Bradford MM, (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
17. Castillon N, Hinnrasky J, Zahm JM, Kaplan H, Bonnet N, et al. (2002) Polarized expression of cystic fibrosis transmembrane conductance regulator and associated epithelial proteins during the regeneration of human airway surface epithelium in three-dimensional culture. Lab Invest 82: 989-998.
18. Hawcroft G, Ko CWS, Hull MA, (2006) Prostaglandin E2-EP4 receptor signalling promotes tumorigenic behaviour of HT-29 human colorectal cancer cells. Oncogene 26: 3006-3019.
19. Motti ML, Califano D, Baldassarre G, Celetti A, Merolla F, et al. (2005) Reduced E-cadherin expression contributes to the loss of p27kip1-mediated mechanism of contact inhibition in thyroid anaplastic carcinomas. Carcinogenesis 26: 1021-1034.
20. Howard EW, Camm KD, Wong YC, Wang XH, (2008) E-cadherin upregulation as a therapeutic goal in cancer treatment. Mini Rev Med Chem 8: 496-518.
21. Jackson AL, Burchard J, Schelter J, Chau BN, Cleary M, et al. (2006) Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity. Rna 12: 1179-1187.
22. Wakefield L, Cornish V, Long H, Griffiths WJ, Sim E, (2007) Deletion of a xenobiotic metabolizing gene in mice affects folate metabolism. Biochem Biophys Res Commun 364: 556-560.
23. Sugamori KS, Wong S, Gaedigk A, Yu V, Abramovici H, et al. (2003) Generation and functional characterization of arylamine N-acetyltransferase Nat1/Nat2 double-knockout mice. Mol Pharmacol 64: 170-179.
24. Cao W, Chau B, Hunter R, Strnatka D, McQueen CA, et al. (2005) Only low levels of exogenous N-acetyltransferase can be achieved in transgenic mice. Pharmacogenomics J 5: 255-261.
25. Hanahan D, Weinberg RA, (2000) The hallmarks of cancer. Cell 100: 57-70.
26. Nelson PJ, Daniel TO, (2002) Emerging targets: molecular mechanisms of cell contact-mediated growth control. Kidney Int 61: S99-105.
27. Cam H, Balciunaite E, Blais A, Spektor A, Scarpulla RC, et al. (2004) A common set of gene regulatory networks links metabolism and growth inhibition. Mol Cell 16: 399-411.
28. Fouquet S, Lugo-Martinez VH, Faussat AM, Renaud F, Cardot P, et al. (2004) Early loss of E-cadherin from cell-cell contacts is involved in the onset of Anoikis in enterocytes. J Biol Chem 279: 43061-43069.
29. Gout S, Marie C, Laine M, Tavernier G, Block MR, et al. (2004) Early enterocytic differentiation of HT-29 cells: biochemical changes and strength increases of adherens junctions. Exp Cell Res 299: 498-510.
30. Onder TT, Gupta PB, Mani SA, Yang J, Lander ES, et al. (2008) Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Cancer Res 68: 3645-3654.
31. Frixen UH, Behrens J, Sachs M, Eberle G, Voss B, et al. (1991) E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells. J Cell Biol 113: 173-185.
32. Mell LK, Meyer JJ, Tretiakova M, Khramtsov A, Gong C, et al. (2004) Prognostic significance of E-cadherin protein expression in pathological stage I-III endometrial cancer. Clin Cancer Res 10: 5546-5553.
33. Oka H, Shiozaki H, Kobayashi K, Inoue M, Tahara H, et al. (1993) Expression of E-cadherin cell adhesion molecules in human breast cancer tissues and its relationship to metastasis. Cancer Res 53: 1696-1701.
34. Perl AK, Wilgenbus P, Dahl U, Semb H, Christofori G, (1998) A causal role for E-cadherin in the transition from adenoma to carcinoma. Nature 392: 190-193.
35. Siitonen SM, Kononen JT, Helin HJ, Rantala IS, Holli KA, et al. (1996) Reduced E-cadherin expression is associated with invasiveness and unfavorable prognosis in breast cancer. Am J Clin Pathol 105: 394-402.
36. Voutilainen KA, Anttila MA, Sillanpaa SM, Ropponen KM, Saarikoski SV, et al. (2006) Prognostic significance of E-cadherin-catenin complex in epithelial ovarian cancer. J Clin Pathol 59: 460-467.
37. Navarro P, Gomez M, Pizarro A, Gamallo C, Quintanilla M, et al. (1991) A role for the E-cadherin cell-cell adhesion molecule during tumor progression of mouse epidermal carcinogenesis. J Cell Biol 115: 517-533.
38. Jeanes A, Gottardi CJ, Yap AS, (2008) Cadherins and cancer: how does cadherin dysfunction promote tumor progression? Oncogene 27: 6920-6929.
39. Sinclair JC, Sandy J, Delgoda R, Sim E, Noble ME, (2000) Structure of arylamine N-acetyltransferase reveals a catalytic triad. Nat Struct Biol 7: 560-564.