RNAi-mediated germline knockdown of FABP4 increases body weight but does not improve the deranged nutrient metabolism of diet-induced obese mice

International Journal of Obesity

Volumn 35, Issue 2, 2011, Pages 217-225

  Yang, R ^a   Castriota, G ^a   Chen, Y ^a   Cleary, M A ^b   Ellsworth, K ^a   Shin, M K ^a   Tran, J L ^a   Vogt, T F ^a   Wu, M ^a   Xu, S ^a   Yang, X ^a   Zhang, B B ^a   Berger, J P ^a   Qureshi, S A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b ROSETTA INPHARMATICS   (United States)

Author keywords

fatty acids; glucose intolerance; insulin resistance; lipids

Indexed keywords

FATTY ACID BINDING PROTEIN 4; GLUCOSE; LIPID;

ADIPOCYTE; ANIMAL EXPERIMENT; ARTICLE; BLOOD ANALYSIS; BODY WEIGHT; CONTROLLED STUDY; GLUCOSE BLOOD LEVEL; GLUCOSE TOLERANCE; INSULIN SENSITIVITY; MACROPHAGE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; WEIGHT GAIN;

ADIPOCYTES; ANIMALS; BODY WEIGHT; ENERGY INTAKE; ENERGY METABOLISM; FATTY ACID-BINDING PROTEINS; GENE KNOCKDOWN TECHNIQUES; GERM-LINE MUTATION; INSULIN RESISTANCE; LIPID METABOLISM; MICE; MICE, KNOCKOUT; MICE, OBESE; NEOPLASM PROTEINS; OBESITY; RNA INTERFERENCE; RNA, MESSENGER;

MUS;

EID: 79951675096     PISSN: 03070565     EISSN: 14765497     Source Type: Journal    
DOI: 10.1038/ijo.2010.128     Document Type: Article

References (20)

1. Furuhashi M, Hotamisligil GS. Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nat Rev Drug Discov 2008; 7: 489-503. (Pubitemid 351767117)
2. Haunerland NH, Spener F. Fatty acid-binding proteins\insights from genetic manipulations. Prog Lipid Res 2004; 43: 328-349. (Pubitemid 38952618)
3. Hertzel AV, Bernlohr DA. The mammalian fatty acid-binding protein multigene family: molecular and genetic insights into function. Trends Endocrinol Metab 2000; 11: 175-180. (Pubitemid 30457137)
4. Hotamisligil GS, Johnson RS, Distel RJ, Ellis R, Papaioannou VE, Spiegelman BM. Uncoupling of obesity from insulin resistance through a targeted mutation in aP2, the adipocyte fatty acid binding protein. Science 1996; 274: 1377-1379. (Pubitemid 26391462)
5. Makowski L, Boord JB, Maeda K, Babaev VR, Uysal KT, Morgan MA et al. Lack of macrophage fatty-acid-binding protein aP2 protects mice deficient in apolipoprotein E against atherosclerosis. Nat Med 2001; 7: 699-705. (Pubitemid 32588026)
6. Tuncman G, Erbay E, Hom X, De Vivo I, Campos H, Rimm EB et al. A genetic variant at the fatty acid-binding protein aP2 locus reduces the risk for hypertriglyceridemia, type 2 diabetes, and cardiovascular disease. Proc Natl Acad Sci USA 2006; 103: 6970-6975.
7. Uysal KT, Scheja L, Wiesbrock SM, Bonner-Weir S, Hotamisligil GS. Improved glucose and lipid metabolism in genetically obese mice lacking aP2. Endocrinology 2000; 141: 3388-3396. (Pubitemid 32269028)
8. Simpson MA, LiCata VJ, Ribarik Coe N, Bernlohr DA. Biochemical and biophysical analysis of the intracellular lipid binding proteins of adipocytes. Mol Cell Biochem 1999; 192: 33-40. (Pubitemid 29171451)
9. Maeda K, Uysal KT, Makowski L, Gorgun CZ, Atsumi G, Parker RA et al. Role of the fatty acid binding protein mal1 in obesity and insulin resistance. Diabetes 2003; 52: 300-307. (Pubitemid 36173182)
10. Cao H, Maeda K, Gorgun CZ, Kim HJ, Park SY, Shulman GI et al. Regulation of metabolic responses by adipocyte/macrophage fatty acid-binding proteins in leptin-deficient mice. Diabetes 2006; 55: 1915-1922. (Pubitemid 44509962)
11. Maeda K, Cao H, Kono K, Gorgun CZ, Furuhashi M, Uysal KT et al. Adipocyte/macrophage fatty acid binding proteins control inte-grated metabolic responses in obesity and diabetes. Cell Metab 2005; 1: 107-119. (Pubitemid 43960591)
12. Furuhashi M, Tuncman G, Gorgun CZ, Makowski L, Atsumi G, Vaillancourt E et al. Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2. Nature 2007; 447: 959-965. (Pubitemid 46975747)
13. Aigner A. Cellular delivery in vivo of siRNA-based therapeutics. Curr Pharm Des 2008; 14: 3603-3619.
14. Yang R, Wilcox DM, Haasch DL, Jung PM, Nguyen PT, Voorbach MJ et al. Liver-specific knockdown ofJNK1 up-regulates proliferator-activated receptor gamma coactivator 1 beta and increases plasma triglyceride despite reduced glucose and insulin levels in diet-induced obese mice. J Biol Chem 2007; 282: 22765-22774. (Pubitemid 47267371)
15. Seibler J, Kuter-Luks B, Kern H, Streu S, Plum L, Mauer J et al. Single copy shRNA configuration for ubiquitous gene knockdown in mice. Nucleic Acids Res 2005; 33: e67.
16. Jiang G, Li Z, Liu F, Ellsworth K, Dallas-Yang Q, Wu M et al. Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1. J Clin Invest 2005; 115: 1030-1038. (Pubitemid 40489408)
17. Furuhashi M, Fucho R, Gorgun CZ, Tuncman G, Cao H, Hotamisligil GS. Adipocyte/macrophage fatty acid-binding pro-teins contribute to metabolic deterioration through actions in both macrophages and adipocytes in mice. J Clin Invest 2008; 118: 2640-2650. (Pubitemid 351949791)
18. Xu H, Barnes GT, Yang Q, Tan G, Yang D, Chou CJ et al. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest 2003; 112: 1821-1830. (Pubitemid 38063705)
19. Hotamisligil GS. Inflammation and metabolic disorders. Nature 2006; 444: 860-867. (Pubitemid 46024993)
20. Coe NR, Simpson MA, Bernlohr DA. Targeted disruption of the adipocyte lipid-binding protein (aP2 protein) gene impairs fat cell lipolysis and increases cellular fatty acid levels. J Lipid Res 1999; 40: 967-972. (Pubitemid 29220539)