Tumor necrosis factor-α represses the expression of NHE2 through NF-κB activation in intestinal epithelial cell model, C2BBe1

Inflammatory Bowel Diseases

Volumn 17, Issue 3, 2011, Pages 720-731

  Amin, Md Ruhul ^a   Orenuga, Temitope ^a   Tyagi, Sangeeta ^a   Dudeja, Pradeep K ^a,b   Ramaswamy, Krishnamurthy ^a   Malakooti, Jaleh ^a,b  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b JESSE BROWN VA MEDICAL CENTER   (United States)

Author keywords

C2BBe1; IBD; inflammation; Na+ uptake; Na+ H+ exchanger; transcriptional regulation

Indexed keywords

AMILORIDE DERIVATIVE; DNA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; MESSENGER RNA; PROTEIN P50; SODIUM PROTON EXCHANGE PROTEIN 2; SODIUM PROTON EXCHANGE PROTEIN 3; SYNAPTOTAGMIN I; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVATION; GEL MOBILITY SHIFT ASSAY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INTESTINE ABSORPTION; INTESTINE EPITHELIUM CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN DNA INTERACTION; PROTEIN EXPRESSION; PROTEIN MOTIF; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION REGULATION; WESTERN BLOTTING;

BLOTTING, WESTERN; CELLS, CULTURED; CHROMATIN IMMUNOPRECIPITATION; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HUMANS; INTESTINAL MUCOSA; INTESTINES; LUCIFERASES; NF-KAPPA B; PROMOTER REGIONS, GENETIC; REPRESSOR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SODIUM-HYDROGEN ANTIPORTER; TRANSFECTION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 79951622528     PISSN: 10780998     EISSN: 15364844     Source Type: Journal    
DOI: 10.1002/ibd.21419     Document Type: Article

References (34)

1. Zachos NC, Tse M, Donowitz M,. Molecular physiology of intestinal Na+/H+ exchange. Annu Rev Physiol. 2005; 67: 411-43. (Pubitemid 40409511)
2. Kiela PR, Ghishan FK,. Ion transport in the intestine. Curr Opin Gastroenterol. 2009; 25: 87-91.
3. Malakooti J, Dahdal RY, Dudeja PK, et al. The human Na(+)/H(+) exchanger NHE2 gene: genomic organization and promoter characterization. Am J Physiol Gastrointest Liver Physiol. 2001; 280: G763-773.
4. Pearse I, Zhu YX, Murray EJ, et al. Sp1 and Sp3 control constitutive expression of the human NHE2 promoter by interactions with the proximal promoter and the transcription initiation site. Biochem J. 2007; 407: 101-111. (Pubitemid 47535585)
5. Malakooti J, Sandoval R, Memark VC, et al. Zinc finger transcription factor Egr-1 is involved in stimulation of NHE2 gene expression by phorbol 12-myristate 13-acetate. Am J Physiol Gastrointest Liver Physiol. 2005; 289: G653-663. (Pubitemid 41377171)
6. Brown K, Gerstberger S, Carlson L, et al. Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation. Science. 1995; 267: 1485-1488.
7. Chen Z, Hagler J, Palombella VJ, et al. Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitin-proteasome pathway. Genes Dev. 1995; 9: 1586-1597.
8. Baeuerle PA, Baichwal VR,. NF-kappa B as a frequent target for immunosuppressive and anti-inflammatory molecules. Adv Immunol. 1997; 65: 111-1137.
9. Ghosh S, Hayden MS,. New regulators of NF-kappaB in inflammation. Nat Rev Immunol. 2008; 8: 837-848.
10. Cominelli F,. Cytokine-based therapies for Crohn's disease-new paradigms. N Engl J Med. 2004; 351: 2045-2048. (Pubitemid 39482497)
11. Clayburgh DR, Musch MW, Leitges M, et al. Coordinated epithelial NHE3 inhibition and barrier dysfunction are required for TNF-mediated diarrhea in vivo. J Clin Invest. 2006; 116: 2682-2694. (Pubitemid 44511632)
12. Rocha F, Musch MW, Lishanskiy L, et al. IFN-gamma downregulates expression of Na(+)/H(+) exchangers NHE2 and NHE3 in rat intestine and human Caco-2/bbe cells. Am J Physiol Cell Physiol. 2001; 280: C1224-1232.
13. Amin MR, Malakooti J, Sandoval R, et al. IFN-gamma and TNF-alpha regulate human NHE3 gene expression by modulating the Sp family transcription factors in human intestinal epithelial cell line C2BBe1. Am J Physiol Cell Physiol. 2006; 291: C887-896. (Pubitemid 44771808)
14. Duckett CS, Perkins ND, Kowalik TF, et al. Dimerization of NF-KB2 with RelA(p65) regulates DNA binding, transcriptional activation, and inhibition by an I kappa B-alpha (MAD-3). Mol Cell Biol. 1993; 13: 1315-1322. (Pubitemid 23068066)
15. Peterson MD, Mooseker MS,. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J Cell Sci. 1992; 102 (Pt 3): 581-600.
16. Moeser AJ, Nighot PK, Ryan KA, et al. Prostaglandin-mediated inhibition of Na+/H+ exchanger isoform 2 stimulates recovery of barrier function in ischemia-injured intestine. Am J Physiol Gastrointest Liver Physiol. 2006; 291: G885-894. (Pubitemid 44772015)
17. Malakooti J, Sandoval R, Amin MR, et al. Transcriptional stimulation of the human NHE3 promoter activity by PMA: PKC independence and involvement of the transcription factor EGR-1. Biochem J. 2006; 396: 327-336. (Pubitemid 43837248)
18. Baud V, Karin M,. Signal transduction by tumor necrosis factor and its relatives. Trends Cell Biol. 2001; 11: 372-377. (Pubitemid 32900529)
19. Xu H, Collins JF, Bai L, et al. Epidermal growth factor regulation of rat NHE2 gene expression. Am J Physiol Cell Physiol. 2001; 281: C504-513.
20. Hawker PC, McKay JS, Turnberg LA,. Electrolyte transport across colonic mucosa from patients with inflammatory bowel disease. Gastroenterology. 1980; 79: 508-511. (Pubitemid 10056397)
21. Rask-Madsen J, Hammersgaard EA, Knudsen E,. Rectal electrolyte transport and mucosal permeability in ulcerative colitis and Crohn's disease. J Lab Clin Med. 1973; 81: 342-353.
22. Martinez-Augustin O, Romero-Calvo I, Suarez MD, et al. Molecular bases of impaired water and ion movements in inflammatory bowel diseases. Inflamm Bowel Dis. 2009; 15: 114-127.
23. Ruiz PA, Braune A, Holzlwimmer G, et al. Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells. J Nutr. 2007; 137: 1208-1215. (Pubitemid 46701913)
24. Erlejman AG, Jaggers G, Fraga CG, et al. TNFalpha-induced NF-kappaB activation and cell oxidant production are modulated by hexameric procyanidins in Caco-2 cells. Arch Biochem Biophys. 2008; 476: 186-195.
25. Schmid RM, Adler G, Liptay S,. Activation of NFkappaB in inflammatory bowel disease. Gut. 1998; 43: 587-588.
26. Ellis RD, Goodlad JR, Limb GA, et al. Activation of nuclear factor kappa B in Crohn's disease. Inflamm Res. 1998; 47: 440-445. (Pubitemid 28532654)
27. Zhu NL, Li C, Huang HH, et al. TNF-alpha represses transcription of human Bone Morphogenetic Protein-4 in lung epithelial cells. Gene. 2007; 393: 70-80. (Pubitemid 46551526)
28. Ashburner BP, Westerheide SD, Baldwin AS Jr,. The p65 (RelA) subunit of NF-kappaB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively regulate gene expression. Mol Cell Biol. 2001; 21: 7065-7077. (Pubitemid 32916079)
29. Hirano F, Tanaka H, Hirano Y, et al. Functional interference of Sp1 and NF-kappaB through the same DNA binding site. Mol Cell Biol. 1998; 18: 1266-1274. (Pubitemid 28100890)
30. Kostadinova RM, Nawrocki AR, Frey FJ, et al. Tumor necrosis factor alpha and phorbol 12-myristate-13-acetate down-regulate human 11beta-hydroxysteroid dehydrogenase type 2 through p50/p50 NF-kappaB homodimers and Egr-1. FASEB J. 2005; 19: 650-652. (Pubitemid 40471274)
31. Chapman NR, Perkins ND,. Inhibition of the RelA(p65) NF-kappaB subunit by Egr-1. J Biol Chem. 2000; 275: 4719-4725. (Pubitemid 30108860)
32. Anderson HD, Rahmutula D, Gardner DG,. Tumor necrosis factor-alpha inhibits endothelial nitric-oxide synthase gene promoter activity in bovine aortic endothelial cells. J Biol Chem. 2004; 279: 963-969. (Pubitemid 38082617)
33. Xu H, Chen H, Dong J, et al. Tumor necrosis factor-{alpha} downregulates intestinal NHE8 expression by reducing basal promoter activity. Am J Physiol Cell Physiol. 2009; 296: C489-497.
34. Sullivan S, Alex P, Dassopoulos T, et al. Downregulation of sodium transporters and NHERF proteins in IBD patients and mouse colitis models: potential contributors to IBD-associated diarrhea. Inflamm Bowel Dis. 2009; 15: 261-274.